Quantitative Immunochemistry of Plant Phosphoenolpyruvate Carboxylases

  • J. Brulfert
  • J. Vidal
Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 4)


Since its discovery (Bandurski and Greiner 1953) phosphoenolpyruvate carboxylase (PEPC, EC has attracted increasing interest among plant scientists. The enzyme catalyses the reaction of CO3H and phosphoenolpyruvate to produce oxaloacetate, immediately reduced to form malate; this latter can be oxidatively decarboxylated by NADP malic enzyme, and thus, appears to be a physiological vector for carbon (CO2) and energy (reducing power). Extensive studies established the ubiquitous presence of PEPC in plants and its functional, regulatory and physico-chemical properties have been described by several groups. PEPC appears to be involved in number of physiological roles, wich were recently extensively reviewed [Physiol Vég 21:5 (1983)]. More particularly PEPC seems to play a fundamental role in adaptation of plant organisms to changes in physiological and environmental parameters; for this reason PEPC can be considered as a good marker for differentiation of physiological processes and for operation of adaptive metabolic pathways. In some cases isoforms involved in specific physiological roles, were described as typical.


Crassulacean Acid Metabolism Pyruvate Carboxylase Phosphoenolpyruvate Carboxylase Immune Serum Cyanogen Bromide 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • J. Brulfert
  • J. Vidal

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