Quantitative Immunochemistry of Plant Phosphoenolpyruvate Carboxylases
Since its discovery (Bandurski and Greiner 1953) phosphoenolpyruvate carboxylase (PEPC, EC 18.104.22.168) has attracted increasing interest among plant scientists. The enzyme catalyses the reaction of CO3H− and phosphoenolpyruvate to produce oxaloacetate, immediately reduced to form malate; this latter can be oxidatively decarboxylated by NADP malic enzyme, and thus, appears to be a physiological vector for carbon (CO2) and energy (reducing power). Extensive studies established the ubiquitous presence of PEPC in plants and its functional, regulatory and physico-chemical properties have been described by several groups. PEPC appears to be involved in number of physiological roles, wich were recently extensively reviewed [Physiol Vég 21:5 (1983)]. More particularly PEPC seems to play a fundamental role in adaptation of plant organisms to changes in physiological and environmental parameters; for this reason PEPC can be considered as a good marker for differentiation of physiological processes and for operation of adaptive metabolic pathways. In some cases isoforms involved in specific physiological roles, were described as typical.
KeywordsCrassulacean Acid Metabolism Pyruvate Carboxylase Phosphoenolpyruvate Carboxylase Immune Serum Cyanogen Bromide
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- Brulfert J, Müller D, Kluge M, Queiroz O (1982b) Photoperiodism and Crassulacean acid metablism I Immunological and kinetic evidences for different patterns of phosphoenolpyru`vate carboxylase isoforms in photoperiodically inducible and non-inducible Crassulacean acid metabolism plants. Planta (Berl) 154: 326–331CrossRefGoogle Scholar
- Crétin C, Perrot-Rechenmann C, Vidal J, Gadal P, Loubinoux B, Tabach S (1983) Study on plant phosphoenolpyruvate carboxylase: sensitivity to herbicides and immunochemical reactivity. Physiol Veg 21: 927–933Google Scholar
- Miginiac-Maslow M, Vidal J, Bismuth E, Hoarau A, Champigny ML (1983) Effets de la carence et la réalimentation en phosphate sur l’équilibre énergétique et l’activité phosphoenolpyruvate carboxylase de jeunes plantes de Blé. Physiol Vég 21: 325–335Google Scholar
- Müller D, Kluge M, Gröschel-Stewart U (1982) Comparative studies in immunological and molecular properties of phosphoenolpyruvate carboxylase in species of Sedum and Kalanchoe performing crassulacean acid metabolism ( CAM ). Plant Cell Environ 5: 223–230Google Scholar
- Nato A, Vidal J (1983) Phosphoenolpyruvate carboxylase activity in relation to physiological processes during the growth of cell suspension cultures from Nicotiana tabacum. Physiol Veg 21: 1031–1039Google Scholar
- Robertson A, Kerr HW (1971) Properties of PEPC isolated from Maize leaves. Biochem J 125: 34Google Scholar
- Vidal J, Godbillon G, Gadal P (1980) Recovery of active, highly purified phosphoenol-pyruvate carboxylase from specific immunoadsorbent column FEBS Lett 118: 31–34Google Scholar
- Vidal J, Crétin C, Gadal P (1983a) The mechanism of photocontrol of phosphoenolpyru-vate carboxylase in sorghum leaves. Physiol Vég 21: 977–986Google Scholar