Radioimmunoassay and Western Blot Analysis of Acyl Carrier Protein Isoforms in Plants

  • T. M. Kuo
  • J. B. Ohlrogge
Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 4)

Abstract

Acyl carrier protein (ACP) is the central cofactor protein to which acyl chains are covalently linked as thioesters during de novo fatty acid synthesis and subsequent elongation and desaturation reactions in plants (Stumpf 1980). In yeast and animals, ACP is an integral part of the multifunctional polypeptide, fatty acid synthase, but in bacteria and plants, ACP is a separate, small polypeptide (ca. 9 kDa MW) which is acidic and heat stable (Majerus and Vagelos 1967; Stumpf 1980; Wakil et al. 1983). Despite this basic organizational difference, the structure of ACP has been highly conserved during evolution as evidenced by greater than 25% homology among the primary sequences of an animal ACP domain, bacterial and plant ACPs (Kuo and Ohlrogge 1984a), and by the interchangeability of bacterial and plant ACPs in several fatty acid biosynthetic reactions (Simoni et al. 1967).

Keywords

HPLC Corn Tyrosine Amide Electrophoresis 

Abbreviations

ACP

acyl carrier protein

RIA

radioimmunoassay

MES

2-(N-morpholino)ethanesulfonic acid

HPLC

high-performance liquid chromatography

DTT

dithiothreitol

OA

Ovalbumin

BSA

bovine serum albumin

PBS

phosphate-buffered saline

PEG

polyethylene glycol

DAF

days after flowering

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • T. M. Kuo
  • J. B. Ohlrogge

There are no affiliations available

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