Radioimmunoassay and Western Blot Analysis of Acyl Carrier Protein Isoforms in Plants
Acyl carrier protein (ACP) is the central cofactor protein to which acyl chains are covalently linked as thioesters during de novo fatty acid synthesis and subsequent elongation and desaturation reactions in plants (Stumpf 1980). In yeast and animals, ACP is an integral part of the multifunctional polypeptide, fatty acid synthase, but in bacteria and plants, ACP is a separate, small polypeptide (ca. 9 kDa MW) which is acidic and heat stable (Majerus and Vagelos 1967; Stumpf 1980; Wakil et al. 1983). Despite this basic organizational difference, the structure of ACP has been highly conserved during evolution as evidenced by greater than 25% homology among the primary sequences of an animal ACP domain, bacterial and plant ACPs (Kuo and Ohlrogge 1984a), and by the interchangeability of bacterial and plant ACPs in several fatty acid biosynthetic reactions (Simoni et al. 1967).
KeywordsFatty Acid Synthesis Castor Bean Acyl Carrier Protein Polytron Homogenizer Antibody Binding Site
acyl carrier protein
high-performance liquid chromatography
bovine serum albumin
days after flowering
Unable to display preview. Download preview PDF.
- Benade L, Ihle JN (1982) Principles of radioimmunoassays and related techniques. In: Marchalonis JJ, and Wan GW (eds) Antibody as a tool. Wiley, New York, pp 163–187Google Scholar
- Majerus PW, Vagelos PR (1967) Fatty acid biosynthesis and the role of the acyl carrier protein. In: Paoletti R, Kritchevsky D (eds) Advances in lipid research, vol 5. Academic Press, New York, pp 1–33Google Scholar
- Stumpf PK (1980) Biosynthesis of saturated and unsaturated fatty acids. In: Stumpf PK (ed) The Biochemistry of plants, vol 4. Academic Press, New York, pp 177–204Google Scholar