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Radioimmunoassay and Gas Chromatography/Mass Spectrometry for Cytokinin Determination

  • Chapter
Immunology in Plant Sciences

Part of the book series: Modern Methods of Plant Analysis ((MOLMETHPLANT,volume 4))

Abstract

During the past few years there has been a rapid increase in the application of modern physicochemical and immunochemical methods to the determination and analysis of plant hormones, especially cytokinins The classical method for cytokinin determination, the bioassay, can no longer be used for unequivocal cytokinin analysis, due to the limited sensitivity and selectivity (Brenner 1981; Weiler 1982; Ernst et al. 1983a). As the accuracy of the bioassay is always open to question, no exact quantification of cytokinins is possible using this method (Reeve and Crozier 1980). Only in searching for detection of new plant hormones is the bioassay invaluable, but their detailed analysis can be better determined by other methods, e.g. gas chromatography/mass spectrometry (GC/MS) or radioimmunoassay (RIA). These modern methods have, for example, found application in the isolation and identification of 6-(o-hydroxybenzylamino)-9-β-d-ribofuranosylpurine in Populus robusta (Horgan et al. 1975), the determination of raphanitin in radish seeds (Summons et al. 1977) and the isolation and identification of 6-(o-hydroxybenzylamino)-2-methylthio-9-β-d-glucofuranosylpurine in Zantedeschia aethiopica (Chaves das Neves and Pais 1980). The identification and quantitation of 6-benzylaminopurine riboside in a cytokinin autotrophic cell culture of anise was also performed by gas chromatography/mass spectrometry using single ion monitoring (SIM) (Ernst et al. 1983b).

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  1. D. Ernst
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  1. Goldberglein 7, D-8520, Erlangen, Deutschland

    Hans-Ferdinand Linskens

  2. Department of Biochemistry, Waite Agricultural Research Institute, University of Adelaide, 5064, Glen Osmond, S.A., Australia

    John F. Jackson

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Ernst, D. (1986). Radioimmunoassay and Gas Chromatography/Mass Spectrometry for Cytokinin Determination. In: Linskens, HF., Jackson, J.F. (eds) Immunology in Plant Sciences. Modern Methods of Plant Analysis, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82853-9_2

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