Analysis of Transcription-Control Signals in Aspergillus

  • C. A. M. J. J. van den Hondel
  • P. J. Punt
  • B. L. M. Jacobs-Meijsing
  • W. van Hartingsveldt
  • R. F. M. van Gorcom
  • P. H. Pouwels
Conference paper
Part of the NATO ASI Series book series (volume 1)

Abstract

Genetic studies in Aspergillus have shown that expression of a number of genes is controlled by trans-acting elements [1,2,3]. These elements may interact with controlling regions located adjacent to structural genes [4, 5]. However, little is known about the molecular mechanisms of gene expression and gene regulation. The recent cloning of genes whose expression is regulated such as the amdS gene [6], trpC gene [7], yA. gene [8] and brlA gene [9] opens the possibility to study gene expression and gene regulation at the molecular level. A powerful and convenient method for such a study is fusion of the gene of interest to the lacZ gene of E. coli [10]. Studies in E. coli [10], Saccharomyces cerevisiae [10] and animal cells [11] have shown that the expression and regulation of genes fused to the lacZ gene can be assayed qualitatively and quantitatively.

Keywords

Proline Arginine Assimilation Tryptophan Aspergillus 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • C. A. M. J. J. van den Hondel
    • 1
  • P. J. Punt
    • 1
  • B. L. M. Jacobs-Meijsing
    • 1
  • W. van Hartingsveldt
    • 1
  • R. F. M. van Gorcom
    • 1
  • P. H. Pouwels
    • 1
  1. 1.Medical Biological Laboratory TNORijswijkThe Netherlands

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