Analysis of Transcription-Control Signals in Aspergillus
Genetic studies in Aspergillus have shown that expression of a number of genes is controlled by trans-acting elements [1,2,3]. These elements may interact with controlling regions located adjacent to structural genes [4, 5]. However, little is known about the molecular mechanisms of gene expression and gene regulation. The recent cloning of genes whose expression is regulated such as the amdS gene , trpC gene , yA. gene  and brlA gene  opens the possibility to study gene expression and gene regulation at the molecular level. A powerful and convenient method for such a study is fusion of the gene of interest to the lacZ gene of E. coli . Studies in E. coli , Saccharomyces cerevisiae  and animal cells  have shown that the expression and regulation of genes fused to the lacZ gene can be assayed qualitatively and quantitatively.
KeywordsProline Arginine Assimilation Tryptophan Aspergillus
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