Analysis of Transcription-Control Signals in Aspergillus
Genetic studies in Aspergillus have shown that expression of a number of genes is controlled by trans-acting elements [1,2,3]. These elements may interact with controlling regions located adjacent to structural genes [4, 5]. However, little is known about the molecular mechanisms of gene expression and gene regulation. The recent cloning of genes whose expression is regulated such as the amdS gene , trpC gene , yA. gene  and brlA gene  opens the possibility to study gene expression and gene regulation at the molecular level. A powerful and convenient method for such a study is fusion of the gene of interest to the lacZ gene of E. coli . Studies in E. coli , Saccharomyces cerevisiae  and animal cells  have shown that the expression and regulation of genes fused to the lacZ gene can be assayed qualitatively and quantitatively.
KeywordsAspergillus Nidulans lacZ Gene lacZ Fusion trpC Gene amdS Gene
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