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Regulation of Steroid Glycoalkaloid and Sesquiterpenoid Stress Metabolite Accumulation in Potato Tubers by Inhibitors of Steroid Synthesis and Phytohormones in Combination with Arachidonic Acid

  • E. C. Tjamos
  • E. Nuckles
  • J. Kuć
Conference paper
Part of the NATO ASI Series book series (volume 1)

Abstract

The polyunsaturated fatty acids, arachidonic (AA) and eicosapentaenoic (EPA), both constituents of Phytophthora infestans, reproduce the hyper-sensitive response when applied to the surface of potato tuber slices [1]. Their effect involves rapid, but localized cell death, browning of the tissue, leakage of electrolytes and foremost, marked inhibition of steroid glycoalkaloid (SGA) accumulation with parallel elicitation of sesquiterpenoid stress metabolites (SSM) accumulation [1,2]. This study refers to the effect of chemicals which inhibit steroid synthesis in fungi and plants such as sterol-inhibiting fungicides [3,4,5] on potato tuber slices. Research concentrated on their effects on SGA and SSM accumulation. The assumption that AA and EPA have a hormonal effect by inhibiting or promoting biochemical processes like phytohormones, justified experimental work on the effect of the main phytohormones indole-3- acetic acid, gibberellic acid and abscisic acid on potato tuber slices. The latter has been reported to affect SSM accumulation in applications with AA [6]. Our aim was to elucidate the mode of action of AA as an elicitor of SSM.

Keywords

Arachidonic Acid Abscisic Acid Potato Tuber Gibberellic Acid Phytophthora Infestans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bostock, R.M., Kuć, J.A. and Laine, R.A., 1981, Eicosapentaenoic and arachidonic acids from Phytophthora infestans elicit fungitoxic sesquiterpenoids in the potato, Science, 212: 67–69.PubMedCrossRefGoogle Scholar
  2. 2.
    Tjamos, E.C. and Kuć, J., 1982, Inhibition of steroid glycoalkaloid accumulation by arachidonic and eicosapentaenoic acids in potato, Science, 217: 542–544.PubMedCrossRefGoogle Scholar
  3. 3.
    Buchenauer, H. and Rohner, E., 1981, Effect of triadimefon and triadimenol on growth of various plant species as well as on gibberellic content and sterol metabolism in shoots of barley seedlings, Pesticide Biochem. Physiol., 15: 58–70.CrossRefGoogle Scholar
  4. 4.
    Schmitt, P. and Benveniste, P., 1979, Effect of fenarimol on sterol biosynthesis in suspension cultures of bramble cells, Phytochemistry, 18: 1659–1665.CrossRefGoogle Scholar
  5. 5.
    Siegel, M.R., 1981, Sterol-inhibiting fungicides:Effects on sterol biosynthesis and sites of action, Plant Diseases, 65: 986–989.CrossRefGoogle Scholar
  6. 6.
    Bostock, R.M., Nuckels, E., Henfling, J.W.D.M. and Kuć, J., 1983, Effects of potato tuber age and storage on sesquiterpenoid stress metabolite accumulation, steroid glycoalkaloid accumulation and response to abscisic and arachidonic acid, Phytopathology, 73: 435–438.CrossRefGoogle Scholar
  7. 7.
    Henfling, J.W.D.M. and Kuć, J., 1979, A semi-micro method for the quantitation of sesquiterpenoid stress metabolites in potato tuber tissue, Phytopathology, 69: 609–612.CrossRefGoogle Scholar
  8. 8.
    Cadle, L.S., Stelzig, D.A., Harper, K.L. and Young, R.J., 1978, Thin layer chromatography system for identification and quantitation of potato tuber glycoalkaloids, J. Agric. Food Chem., 26: 1453–1454.PubMedCrossRefGoogle Scholar
  9. 9.
    Henfling, J.W.D.M., Bostock, R. and Kuć, J., 1979, Effect of abscisic acid on rishitin and lubimin accumulation and resistance to Phytophthora infestans and Cladosporium cucumerinum in potato tuber tissue slices, Phytopathology, 70: 1074–1078.CrossRefGoogle Scholar
  10. 10.
    Chrispeels, M.J. and Varner, J.E., 1966, Inhibition of gibberellic acid induced formation of α-amylase by abscisic II, Nature, 212: 1066–67.CrossRefGoogle Scholar
  11. 11.
    Darvill, A.G. and Albersheim, P., 1984, Phytoalexins and their elicitors -A defense against microbial infection in plants, Annu. Rev. Plant Physiol., 35: 243–275.CrossRefGoogle Scholar
  12. 12.
    Agnew, W.S. and Popjak, G., 1978, Squalene synthetase: Stoichiometry and kinetics of presqualene pyrophosphate and squalene synthesis by yeast microsomes, J. Biol. Chem., 253: 4566–4573.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • E. C. Tjamos
    • 1
  • E. Nuckles
    • 2
  • J. Kuć
    • 2
  1. 1.Benaki Phytopathological InstituteAthensGreece
  2. 2.Department of Plant PathologyUniversity of KentuckyLexingtonUSA

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