Imaging Intracellular Elemental Distribution and Ion Fluxes in Cryofractured, Freeze-Dried Cultured Cells Using Ion Microscopy
Elements play an important role in intracellular regulatory events. Cells grown in cultures provide an excellent model for studying the distribution and transport of ions under normal and pathological conditions. The unique ability of the ion microscope to provide ion images with cell morphology is ideally suited to such studies. However, analysis of cultured cells at the subcellular level has been problematic in ion microscopy (1,2,3). Due to the highly diffusible nature of physiologically important elements (Na, K, Ca, etc.), cryo-techniques are the only acceptable means of sample preparation. Using a cryo-fracture technique, we have been able to study subcellular elemental distribution in several cell lines.
KeywordsStandard Tissue Culture Acceptable Means Mouse Fibroblast Culture Nitrogen Slush Unsynchronized Cell
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