Imaging Intracellular Elemental Distribution and Ion Fluxes in Cryofractured, Freeze-Dried Cultured Cells Using Ion Microscopy
Elements play an important role in intracellular regulatory events. Cells grown in cultures provide an excellent model for studying the distribution and transport of ions under normal and pathological conditions. The unique ability of the ion microscope to provide ion images with cell morphology is ideally suited to such studies. However, analysis of cultured cells at the subcellular level has been problematic in ion microscopy (1,2,3). Due to the highly diffusible nature of physiologically important elements (Na, K, Ca, etc.), cryo-techniques are the only acceptable means of sample preparation. Using a cryo-fracture technique, we have been able to study subcellular elemental distribution in several cell lines.
Unable to display preview. Download preview PDF.
- (1).R.W. Linton, S.R. Walker, C.R. DeVries, P. Ingram, J.D. Shelburne: Scanning Electron Microsc. II, 583 (1980).Google Scholar
- (2).R.W. Linton, M.E. Farmer, Ingram, S.R. Walker, J.D. Shelburne: Scanning Electron Microsc. III, 1191 (1982).Google Scholar
- (3).S. Chandra, G.H. Morrison; “Cell Cultures: An Alternative in Biological Ion Mass Spectrometry SIMS IV; Benninghoven, A.; Okana, J.; Shimizu, R.; Werner, H.W., Eds.; Springer-Verlag: New York, 1984; pp. 489–491.Google Scholar
- (4).S. Chandra, G.H. Morrison, C.C. Wolcott. J. Cell Biol. (submitted).Google Scholar