Fundamental Aspects

Abstract

The first plasmid detected in a eukaryote was found in a strain of baker’s yeast, Saccharomyces cerevisiae almost two decades ago (Sinclair et al. 1967). Its existence was revealed initially by electron microscopic analyses, a major screening technique for extrachromosomal genetic traits in those days. Based on contour length measurements from electron micrographs, this plasmid was termed “2 μm DNA”. Further details of this initially recognized eukaryotic plasmid will be discussed in the next chapter (p. 13). However, the following biological properties of the 2 μm plasmid are particularly noteworthy:

• 1. Almost without exception, every strain of S. cerevisiae and closely related yeasts contain the 2 μm plasmid. Strains investigated include wild strains isolated from nature as well as laboratory and industrial strains (see Table l).

• 2. The 2 μm plasmid is a multicopy plasmid located within the nuclear membrane and is now considered to be a “minichromosome” (p. 14).

• 3. Unlike the prokaryotic plasmids first discovered, specific functions cannot be attributed to the 2 μm plasmid. Like many of the eukaryotic plasmids, it seems to be cryptic.

• 4. The 2 μm plasmid has been used to develop the first eukaryotic transformation system (p.65ff.)