Regulation of Hormone Production in Small Cell Carcinoma of the Lung

  • G. D. Sorenson
  • C. C. Cate
  • O. S. Pettengill
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 99)


Patients with pulmonary carcinoma frequently have elevated levels of one or more circulating tumor-produced hormones. This is particularly characteristic of small cell carcinoma of the lung (SCCL), where an increased frequency of hormone production is seen more commonly than in other types of lung cancer. The most frequently elevated hormones in such patients are calcitonin (CT), ACTH and neurophysins (Sorenson et al. 1984a). In addition to the noteworthy frequency of hormone production by tumors in these patients, there is also a wide variety of hormones produced by individual SCCL tumors. The reasons for the common occurrence and the great variety of hormones produced by SCCL are unknown, as are the molecular mechanisms involved.


Small Cell Carcinoma Hormone Production AtT20 Cell Pulmonary Carcinoma Calcitonin Secretion 
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  1. Abe K, Kameya T, Yamguchi K, Kikuchi K, Adachi I, Tanaka M, Kimura S, Kodama T, Shimosato Y, Ishikawa S (1984) Hormone-producing lung cancers. Endocrinologic and morphologic studies. In: Becker KL, Gazdar AF (eds) The endocrine lung in health and disease. Saunders, Philadelphia, pp 549–595.Google Scholar
  2. Amara SG, Jonas V, Rosenfeld MG, Ong ES, Evans RM (1982) Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature 298: 240–244.PubMedCrossRefGoogle Scholar
  3. Becker KL, Managhan KG, Silva OL (1980) Immunocytochemical localization of calcitonin in Kulchitsky cells of human lung. Arch Pathol Lab Med 104: 196–198.PubMedGoogle Scholar
  4. Becker KL, Gazdar AF, Carney DN, Snider RH, Moore CF, Silva OL (1983) Calcitonin secretion by continuous cultures of small cell carcinoma of the lung: incidence and immuno-heterogeneity studies. Cancer Lett 18: 174–185.CrossRefGoogle Scholar
  5. Becker KL, Silva OL, Gazdar AF, Snider RH, Moore CT (1984) Calcitonin and small cell cancer of the lung. In: Becker KL, Gazdar AF (eds) The endocrine lung in health and disease. Saunders, Philadelphia, pp 528–548.Google Scholar
  6. Bishop JM (1983) Cellular oncogenes and retroviruses. Ann Rev Biochem 52: 301–354.PubMedCrossRefGoogle Scholar
  7. Bittner M, Kupferer P, Morris CF (1980) Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets. Anal Biochem 102: 459–471.PubMedCrossRefGoogle Scholar
  8. Busslinger M, Hurst J, Flavell RA (1983) DNA methylation and the regulation of globin gene expression. Cell 34: 197–206.PubMedCrossRefGoogle Scholar
  9. Cate RL, Chick W, Gilbert W (1983) Comparison of the methylation patterns of the two rat insulin genes. J Biol Chem 258: 6645–6652.PubMedGoogle Scholar
  10. Cate CC, Douple EB, Andrews KM, Pettengill OS, Curphey TJ, Sorenson GD, Maurer LH (1984) Calcitonin as an indicator of the response of human small cell carcinoma of the lung cells to drugs and radiation. Cancer Res 44: 949–954.PubMedGoogle Scholar
  11. Cutz E, Chan W, Track, NS (1981) Bombesin, calcitonin and leu-enkephalin immunoreactivity in endocrine cells of the human lung. Experientia 37: 765–767.PubMedCrossRefGoogle Scholar
  12. Debold CR, Schworer ME, Connor TB, Bird RE, Orth DN (1983) Ectopic pro-opiomelanocortin: sequence of cDNA coding for beta-melanocyte stimulating hormone and beta-endorphin. Science 220: 721–723.PubMedCrossRefGoogle Scholar
  13. Delprete S, Pettengill OP, Cate CC, Ghatei M, Bloom SB, Sorenson GD (1985) Bombesin and calcitonin secretion by pulmonary carcinoma is stimulated by histaminic agonists (submitted for publication).Google Scholar
  14. Downward J, Yarden Y, Mayes E, Scrace G, Totty N, Stockwell P, Ullrich A, Schlessinger J, Waterfield MD (1984) Close similarity of epidermal growth factor receptor and V-erb-B oncogene protein sequences. Nature 307: 521–527.PubMedCrossRefGoogle Scholar
  15. Feinberg AP, Vogelstein B (1983) Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature 301: 89–92.PubMedCrossRefGoogle Scholar
  16. Felsenfeld G, McGhee J (1982) Methylation and gene control. Nature 196: 602–603.CrossRefGoogle Scholar
  17. Fox M (1984) Gene amplification and drug resistance. Nature 307: 212–213.PubMedCrossRefGoogle Scholar
  18. Garel A, Axel R (1976) Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei. Proc Natl Acad Sci USA 73: 3966–3970.PubMedCrossRefGoogle Scholar
  19. Groudine M, Weintraub H (1980) Activation of cellular genes by avian RNA tumor viruses. Proc Natl Acad Sci USA 77: 5351–5354.PubMedCrossRefGoogle Scholar
  20. Groudine M, Weintraub H (1982) Propagation of globin DNAse I hyposensitive sites in absence of factors required for induction: A possible mechanism for determination. Cell 30: 131–137.PubMedCrossRefGoogle Scholar
  21. Gumbiner B, Kelly RB (1982) Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells. Cell 28: 51–59.PubMedCrossRefGoogle Scholar
  22. Hanamia N, Shaool D, Harel J, Wiels J, Tursz T (1983) Common multigenic activation in different human neoplasias. EMBO 2: 1621–1624.Google Scholar
  23. Lehrach H, Diamond D, Wozney JM, Boedtker H (1979) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16: 4743–4751.CrossRefGoogle Scholar
  24. Lewin B (1983) Genes. Wiley, New York.Google Scholar
  25. Little CD, Nau MM, Carney DN, Gazdar AF, Minna JD (1983) Amplication and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194–196.PubMedCrossRefGoogle Scholar
  26. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
  27. Mathis D, Oudet P, Chambon P (1980) Structure of transcribing chromatin. Prog in Nucleic Acid Res Mol Biol 24: 1–55.CrossRefGoogle Scholar
  28. Moore HP, Gumbiner B, Kelly RB (1983) Chloroquine diverts ACTH from a regulated to a constitutive secretory pathway in AtT-20 cells. Nature 302: 434–436.PubMedCrossRefGoogle Scholar
  29. Murdock GH, Potter E, Nicolaisen AK, Evans RM, Rosenfeld MG (1982 a) Epidermal growth factor rapidly stimulates prolactin gene transcription. Nature 300: 192–194.CrossRefGoogle Scholar
  30. Murdock GH, Rosenfeld MG, Evans RM (1982 b) Eukaryotic transcriptional regulation and chromatin associated protein phosphorylation by cyclic AMP. Science 218: 1315–1317.CrossRefGoogle Scholar
  31. Naylor SL, Sakuguchi AY, Szoka P, Hendy GN, Kronenberg HM, Shows TB (1983 a) Human parathyroid hormone gene (pth) is on the short arm of chromosome 11. Somatic Cell Genet 9: 609–616.PubMedCrossRefGoogle Scholar
  32. Naylor SL, Sakaguchi AY, Shen L-P, Bell GI, Rutler WJ, Shows TB (1983 b) Polymorphic human somotostatin gene is located in chromosome 3. Proc Natl Acad Sci USA 80: 2686–2689.PubMedCrossRefGoogle Scholar
  33. Pettengill OS, Sorenson GD (1981) Tissue culture and in vitro characteristics, In: Greco FA, Oldham RK, Bunn PA (eds) Small cell lung cancer. Grune and Stratton, New York, pp 51–77.Google Scholar
  34. Pettengill OS, Faulkner CS, Wurster-Hill DH, Maurer LH, Sorenson GD, Robinson A, Zimmerman EA (1977) Isolation and characterization of a hormone producing cell line from human small cell anaplastic carcinoma of the lung. J Natl Cancer Inst 58: 511–518.PubMedGoogle Scholar
  35. Pettengill OS, Curphey TJ, Cate CC, Flint CT, Maurer LH, Sorenson GD (1980 a) Animal model for small cell carcinoma of the lung: effect of immunosupression and sex of mouse on tumor growth in nude athymic mice. Exp Cell Biol 48: 270–297.Google Scholar
  36. Pettengill OS, Sorenson GD, Wurster-Hill DH, Curphey TJ, Noll WW, Cate CC, Maurer LH (1980 b) Isolation and growth characteristics of continuous cell lines from small cell carcinoma. Cancer 45: 906–918.PubMedCrossRefGoogle Scholar
  37. Przepiorka D, Baylin SB, McBride OW, Testa JR, deBustros A, Nelkin BD (1984) The human calcitonin gene is located on the short arm of chromosome 11. Biochem Biophys Res Commun 120: 493–499.PubMedCrossRefGoogle Scholar
  38. Razin A, Friedman J (1981) DNA methylation and its possible biological roles. Prog Nucleic Acid Res Mol Biol 25: 33–52.PubMedCrossRefGoogle Scholar
  39. Robbins DC, Tager HS, Rubenstein AH (1984) Biologic and clinical importance of proinsulin. N Engl J Med 310: 1165–1175.PubMedCrossRefGoogle Scholar
  40. Schimke RT (ed) (1982) Gene amplification. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
  41. Shows TB, Sakaguichi AY, Naylor SL (1982) Mapping the human genome, cloned genes, DNA polymorphisms and inherited disease. Adv Human Genet 12: 341–451.CrossRefGoogle Scholar
  42. Sorenson GD, Pettengill OS (1980) Structural and functional characteristics of small cell carcinoma of the lung, in vitro and in vivo. Biol Cell 39: 277–280.Google Scholar
  43. Sorenson GD, Pettengill OS, Brinck-Johnsen T, Cate CC, Maurer LH (1981) Hormone production by cultures of small cell carcinoma of the lung. Cancer 47: 1289–1296.PubMedCrossRefGoogle Scholar
  44. Sorenson GD, Bloom SR, Ghatei MA, Cate CC, Pettengill OS (1982) Bombesin production by human small cell carcinoma of the lung. Regul Pept 4: 59–66.PubMedCrossRefGoogle Scholar
  45. Sorensen GD, Pettengill OS, Brown SJ, Bacopoulos NG (1983 a) Evidence for muscarinic cholinergic receptors in cultured human small cell carcinoma of the lung. Fed Proc 42: 388.Google Scholar
  46. Sorenson GD, Pettengill OS, Cate CC, Gosselin EJ, Ghatei MA, Bloom SR (1983 b) Bombesin and calcitonin secretion by pulmonary carcinoma is modulated by cholinergic receptors. Life Sci 33: 1939–1944.PubMedCrossRefGoogle Scholar
  47. Sorensen GD, Pettengill OS, Cate CC, Delprete SA (1984 a) Biomarkers in small cell carcinoma of the lung. In: Aisner J (ed) Lung cancer. Livingstone, New York.Google Scholar
  48. Sorenson GD, Pettengill OS, Cate CC, Ghatei MA, Bloom SR (1984 b) Modulation of bombesin and calcitonin secretion in cultures of small cell carcinoma of the lung. In: Bedker KL, Gazdar AF (eds) The endocrine lung in health and disease. Saunders, Philadelphia, pp 596–602.Google Scholar
  49. Stellway ES, Dahlberg AE (1980) Electrophoretic transfer of DNA, RNA and protein onto diazo-benzyloxymethyl (DBM) paper. Nucleic Acid Res 8: 299–317.CrossRefGoogle Scholar
  50. Suzuki H, Tsutsumi Y, Yamaguchi K, Abe K, Yokoyama T (1984) Small cell lung carcinoma with ectopic adrenocorticotropic hormone and antidiuretic hormone syndromes. J Clin Oncol 14: 129–137.Google Scholar
  51. Weisbrod S (1982) Active Chromatin. Nature 297: 289–295.PubMedCrossRefGoogle Scholar
  52. Wharton J, Polak JM, Bloom SR, Ghatei MA, Solcia E, Brown MR, Pearse AGE (1978) Bombesin-like immunoreactivity in the lung. Nature 273: 769–770.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • G. D. Sorenson
    • 1
  • C. C. Cate
    • 1
  • O. S. Pettengill
    • 1
  1. 1.Department of PathologyDartmouth Medical SchoolHanoverUSA

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