Parathyroid Hormone and PTHmRNA in a Human Small Cell Lung Cancer

  • H.-J. Schmelzer
  • R.-D. Hesch
  • H. Mayer
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 99)


Hypercalcemia is often associated with malignant diseases. The frequency of ectopic production of PTH as a cause of humoral hypercalcemia in association with malignant diseases is controversial. Some studies have suggested that patients with tumors and hypercalcemia have elevated iPTH in the serum or in tumor extracts (Sherwood et al. 1967; Benson et al. 1974), whereas others have been unable to detect iPTH in either tumor extracts or serum (Powell et al. 1973). Further studies have shown that humoral hypercalcemia is due to an altered form of PTH, which is unrecognized or only partially recognized by the radioimmunoassay (RIA) for PTH but which is still able to bind to the PTH receptor and is measurable in an in vitro bioassay (Goltzman et al. 1981). Up to now it has not been possible to distinguish between de novo synthesis of PTH by the ectopic tumor cells and the accumulation of exogenous PTH in the tumor tissue.


Parathyroid Hormone Small Cell Lung Carcinoma Translation Product Tumor Extract Human Small Cell Lung Cancer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Antonavakis SE, Phillips JA III, Mallonee RL, Karzazian HH JR, Fearon ER, Waber PG, Kronenberg HM, Ullrich A, Meyers DA (1983) β-Globin locis is linked to the parathyroid hormone (PTH) locus and lies between the insulin and PTH loci in man. Proc Natl Acad Sci USA 80: 6615–6619.CrossRefGoogle Scholar
  2. Aurbach GP (1959) Isolation of parathyroid hormone after extraction with phenol. J Biol Chem 234: 3179–3181.PubMedGoogle Scholar
  3. Benson RC Jr, Riggs BL, Pickard BM, Arnaud CD (1974) Radioimmunoassay of parathyroid hormone in hypercalcemia patients with malignant disease. Ann J Med 56: 821–826.CrossRefGoogle Scholar
  4. Goltzman D, Stewart AF, Broadus AE (1981) Malignancy-Associated hypercalcemia: evaluation with a cytochemical bioassay for parathyroid hormone. J Clin Endocrinol Metab 53: 899–904.PubMedCrossRefGoogle Scholar
  5. Habener JF, Rosenblatt M, Kemper B, Kronenberg HM, Rich A, Potts JT Jr (1980) Pre-pro parathyroid hormone: amino acid sequence, chemical synthesis, and some biological studies of the precursor region. Proc Natl Acad Sci USA 75: 2616–2620.CrossRefGoogle Scholar
  6. Hehrmann R, Nordmeyer H, Wilke R, Hesch RD (1977) Radioimmunoassay of hPTH; characterisation of two new antisera from sheep and clinical results. Acta Endocrinol (Copenh) [Suppl] 208: 117–118.Google Scholar
  7. Hu CP, Slate DL, Gravel R, Ruddle FH (1979) Biological detection of specific mRNA molecules by microinjection. Proc Natl Acad Sci USA 76: 4503–4506.CrossRefGoogle Scholar
  8. Ibbotson KJ, D’Sonza SM, Ng KW, Osborne CK, Niall M, Martin TJ, Mundy GR (1983) Tumor-derived growth factor increases bone resorption in a tumor associated with humoral hypercalcemia of malignancy. Science 221: 1292–1294.PubMedCrossRefGoogle Scholar
  9. MacSween JM, Eastwood SL (1981) Recovery of antigen from staphylococcal protein A — antibody adsorbens. Methods Enzymol 73: 459–471.PubMedCrossRefGoogle Scholar
  10. Mayer H, Breyel E, Bostock C, Schmidtke J (1983) Assignment of the human parathyroid hormone gene to chromosome 11. Hum Genet 64: 283–285.PubMedCrossRefGoogle Scholar
  11. Mundy GR, Raisz LG, Cooper RA, Schechter GP, Salmon SE (1974) Evidence for secretion of an osteoclast stimulating factor in myeloma. N Engl J Med 291: 1041–6.PubMedCrossRefGoogle Scholar
  12. Powell D, Singer FR, Murray TM, Minkin C, Potts JT Jr (1973) Nonparathyroid humoral hypercalcemia in patients with neoplastic diseases. N Engl J Med 289: 176–181.PubMedCrossRefGoogle Scholar
  13. Roberts BE, Paterson BM (1973) Efficient translation of Tobacco mosaic virus RNA and rabbit globin 9 S RNA in a cellfree system from commercial wheat germ. Proc Natl Acad Sci USA 70: 2330–2334.PubMedCrossRefGoogle Scholar
  14. Sherwood LM, O’Riordan JLH, Aurbach GD, Potts JT Jr (1967) Production of parathyroid hormone by nonparathyroid tumors. J Clin Endocrinol Metab 27: 140–146.PubMedCrossRefGoogle Scholar
  15. Simpson EL, Mundy GR, D’Sonza SM, Ibbotson KJ, Bockmann R, Jacobs JW (1983) Absence of parathyroid hormone messenger RNA in nonparathyroid tumors associated with hypercalcemia. N Engl J Med 309: 325–330.PubMedCrossRefGoogle Scholar
  16. Tashjian AH Jr, Voelkel EF, Levine L, Goldhaber P (1972) Evidence that the bone resorption-stimulating factor produced by mouse fibrosarcoma cells is prostaglandin E2 a new model for the hypercalcemia of cancer. J Exp Med 136: 1329–1343.PubMedCrossRefGoogle Scholar
  17. Vasicek TJ, McDevitt BE, Freeman MW, Fennick BJ, Hendy GN, Potts JT Jr, Rich A, Kronenberg HM (1983) Nucleotide sequence of the human parthyroid hormone gene. Proc Natl Acad Sci USA 80: 2127–2131.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • H.-J. Schmelzer
    • 1
  • R.-D. Hesch
    • 1
  • H. Mayer
    • 1
  1. 1.Gesellschaft für Biotechnologische Forschung mbHMascheroder WegBraunschweigDeutschland

Personalised recommendations