Growth and Cellular Characteristics of Multicell Spheroids

  • R. M. Sutherland
  • R. E. Durand
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 95)


Experience with culture methods for multicell spheroids has demonstrated the importance of optimizing and standardizing the growth conditions for different cell types and research objectives. Spheroid growth kinetics, cellular properties, and microenvironmental characteristics are significantly influenced by spheroid cellular metabolism and the macroenvironmental conditions during the culture period. These conditions include constituents of the culture medium, frequency of replenishment of the medium, the oxygen and glucose concentrations and geometric and convection properties in the culture flask.


Tumor Spheroid Cellular Characteristic Spinner Flask Multicellular Spheroid Multicellular Tumor Spheroid 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • R. M. Sutherland
    • 1
  • R. E. Durand
    • 1
  1. 1.Experimental Therapeutics Division, Department of Radiation Biology and BiophysicsUniversity of Rochester Cancer CenterRochesterUSA

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