Abstract
A number of technical problems prevent the widespread use of in vitro clonal assays for human tumor cells on a routine clinical basis (Selby et al. 1983). One area where improvements can be expected is in the culture conditions, since a major limitation of these assays is the low plating efficiencies (PEs) which are usually obtained. This, of course, means that anticancer drugs can be individually tested only on a minority of tumor samples. Furthermore, tumor colony growth in most assay cultures is dependent on the presence of animal sera, which contain variable amounts of various nutrients, hormones, and growth factors. Standardization of culture conditions between laboratories or even between serum batches is, therefore, impossible.
Keywords
- Colony Formation
- Melanoma Cell Line
- Clonogenic Assay
- Plating Efficiency
- Bovine Serum Albumin Concentration
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This work was supported by grants from the Swiss National Foundation for Scientific Research, the Swiss League Against Cancer, and the Fonds de Recherche sur les Lymphomes Malins
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© 1984 Springer-Verlag Berlin · Heidelberg
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Eliason, J.F., Fekete, A., Odartchenko, N. (1984). Improving Techniques for Clonogenic Assay. In: Hofmann, V., Berens, M.E., Martz, G. (eds) Predictive Drug Testing on Human Tumor Cells. Recent Results in Cancer Research, vol 94. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82295-7_29
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DOI: https://doi.org/10.1007/978-3-642-82295-7_29
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