Heterogeneity and Variability of Test Results as Limiting Factors for Predictive Assays
A number of different human neoplasms have been postulated to arise from more than one single cell of origin (Ohno 1971; Petersen et al. 1979; Siracky 1979), whereas there is evidence that myelomas and some hematological malignancies may be monoclonal (Fialkow 1976). The question arises whether the wellknown morphologically different cell types found in many tumors point to a multicellular origin or whether these phenotypes only represent differentiation levels or directions of cells which have developed from a single stem cell (Nowell 1976).
KeywordsClonogenic Assay Cloning Efficiency Predictive Assay Single Stem Cell Tumor Cell Subpopulation
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