Advertisement

Short-Term In Vitro Sensitivity Testing in Acute Leukemia

  • J. D. Schwarzmeier
  • R. Pirker
  • E. Paietta
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 94)

Abstract

The treatment of acute leukemia with highly cytotoxic drug regimens has made well-planned protocols necessary in order to avoid undue exposure of the patients. It has therefore become more and more desirable to find determinant initial factors related to possibly good or poor responses of the patients to cytostatic therapy. Numerous investigators have attempted to establish such factors and have stressed the role of pretreatment cytokinetics, leukemic cell mass, cytologic criteria, chromosomal aberra­tions, etc. Previous studies in our laboratory have emphasized the predictive implications of cyclic adenosine monophosphate in leukemic cells and have reconfirmed the prognostic value of proliferation kinetics (Paietta et al. 1980). A more direct approach to detect sensitivity of leukemic blast cells, however, is their in vitro exposure to specific chemotherapeutic agents and the determination of reliable parameters of cell viability (Dow 1980; Preisler 1980; Spiro et al. 1981; Von Hoff and Weisenthal 1980; Weisenthal 1981). Since the course of the disease in patients with acute leukemia very often requires prompt therapeutic decisions, the application of short-term tests seems to be more feasible than the use of time-consuming but perhaps more sensitive assays. We therefore investigated the in vitro effect of various cytostatic agents on the incorporation of nucleoside precursors into leukemic blast cells and correlated the in vitro results with the clinical response of the patients to chemotherapy.

Keywords

Acute Myelocytic Leukemia Acute Lymphocytic Leukemia Acute Leukemia Blast Cell Raji Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Dosik GM, Barlogie B, Johnston D, Mellard D, Freireich EJ (1981) Dose-dependent suppression of DNA snythesis in vitro as a predictor of clinical response in adult myeloblastic leukemia. Eur J Cancer 17: 549–555PubMedGoogle Scholar
  2. Dow LW (1980) Sensitivity of normal and neoplastic cells to chemotherapeutic agents in vitro. Adv Intern Med 25: 427–452PubMedGoogle Scholar
  3. Group for Sensitivity Testing of Tumors (KSST) (1981) In vitro short term test to determine the resistance of human tumors to chemotherapy. Cancer 48: 2127–2135Google Scholar
  4. Paietta E, Mittermayer K, Schwarzmeier JD (1980) Proliferation kinetics and cyclic AMP as prognostic factors in adult acute leukemia. Cancer 46: 102–108PubMedCrossRefGoogle Scholar
  5. Raich PC (1978) Prediction of therapeutic response in acute leukaemia. Lancet 1: 74–76PubMedCrossRefGoogle Scholar
  6. Sanfilippo O, Silvestrini RS, Daidone MG (1982) In vitro anitmetabolic assay for the prediction of human tumor chemosensitivity. Proc ASCO 1: 24Google Scholar
  7. Schwarzmeier JD, Paietta E, Mittermayer K, Pirker R (1984) Prediction of the response to chemotherapy in acute leukemia by a short term test in vitro. Cancer 53: 390–395PubMedCrossRefGoogle Scholar
  8. Spiro TE, Mattelaer MA, Efira A, Stryckmans P (1981) Sensitivity of myeloid progenitor cells in healthy subjects and patients with chronic myeloid leukemia to chemotherapeutic agents. J Natl Cancer Inst 66: 1053–1059PubMedGoogle Scholar
  9. Vo1m M, Kaufmann M, Hinderer H, Goerttler K (1970) Schnellmethode zur Sensibilitätstestung maligner Tumoren gegenüber Cytostatika. Klin Wochenschr 48: 374–376PubMedCrossRefGoogle Scholar
  10. Vo1m M, Kaufmann M, Mattem J, Wayss K (1975) Möglichkeiten und Grenzen der prätherapeutischen Sensibilitätstestung von Tumoren gegen Zytostatika im Kurzzeittest. Schweiz Med Wochenschr 105: 74–82PubMedGoogle Scholar
  11. Von Hoff DD, Weisenthal L (1980) In vitro methods to predict for patient response to chemotherapy. Adv Pharmacol Chemother 17: 133–156CrossRefGoogle Scholar
  12. Weisenthal LM (1981) In vitro assays in preclinical antineoplastic drug screening. Semin Oncol 8: 362–376PubMedGoogle Scholar
  13. Zittoun R, Bouchard M, Facquet-Danis J, Percie-du-Sert M, Bousser J (1975) Prediction of the response to chemotherapy in acute leukemia. Cancer 35: 507–513PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1984

Authors and Affiliations

  • J. D. Schwarzmeier
    • 1
  • R. Pirker
    • 1
  • E. Paietta
    • 1
  1. 1.I. Medizinische UniversitätsklinikWienAustria

Personalised recommendations