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Laser Desorption Mass Spectrometry of Complex Biomolecules at High Laser Power Density

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Secondary Ion Mass Spectrometry SIMS IV

Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 36))

Abstract

The laser microprobe mass analyzer LAMMA® 500 originally designed for the extremely sensitive tracing of elements and molecules in microsamples and tissue obviously comprises soft ionization characteristics allowing the mass spectrometric analysis of ther-molabile, non-volatile high-molecular-weight biomolecules [1] . The possibilities and limitations of getting information on molecular weight and chemical structure are demonstrated with the aid of positive and negative ion LD mass spectra of two free lipid A samples isolated from Proteus mirabilis bacteria which were grown at different temperatures. Furthermore, a model of the laser-induced desorption process from organic solids is derived based on the experimental experience with LAMMA 500 applied to a large number of chemically different compounds and on the influence of laser power density and sample conditions (geometry, compactness).

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References

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Author information

Authors and Affiliations

  1. Forschungsinstitut Borstel, Borstel, D-2061, Fed. Rep. of Germany

    B. Lindner & U. Seydel

Authors
  1. B. Lindner
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  2. U. Seydel
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Editor information

Editors and Affiliations

  1. Phsysikalisches Inst., Universität Münster, Münster, D-4400, Fed. Rep. of Germany

    A. Benninghoven

  2. College of General Education, Osaka University, Osaka 560, Japan

    J. Okano

  3. Department of Applied Physics, Osaka University, Osaka 565, Japan

    R. Shimizu

  4. Philips Research Laboratories, Eindhoven, The Netherlands

    H. W. Werner

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© 1984 Springer-Verlag Berlin Heidelberg

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Lindner, B., Seydel, U. (1984). Laser Desorption Mass Spectrometry of Complex Biomolecules at High Laser Power Density. In: Benninghoven, A., Okano, J., Shimizu, R., Werner, H.W. (eds) Secondary Ion Mass Spectrometry SIMS IV. Springer Series in Chemical Physics, vol 36. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82256-8_95

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  • DOI: https://doi.org/10.1007/978-3-642-82256-8_95

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-82258-2

  • Online ISBN: 978-3-642-82256-8

  • eBook Packages: Springer Book Archive

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