Abstract
All current techniques (autoradiography excepted) for the detection of nuclear or cytosol steroid hormone receptors require homogenization of tissue, and thereby sophisticated equipment not available at all centers. Several methods have been developed to trace estrogen (ER) and progesterone receptors (PgR) under the microscope (see [2] for review). In this regard the ligands employed so far consist of:
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1.
Steroid hormones linked to a carrier molecule (bovine serum albumin) which is labeled with fluorescent dyes (∼2 × 10-4 M effective bound steroids) [10, 12]
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2.
Fluorescein-labeled estrogen molecule, without a protein carrier (∼ 10-6 M bound steroid) [5, 14]
This work was supported by the Fonds Cancérologique de la CGER (Belgium) and the FRSM (Belgium). We gratefully acknowledge the cooperation of Prof. C. Gompel (Service d’Anatomie Pathologique, Institut J. Bordet, Bruxelles, Belgium). We thank Miss P. Miroir for her excellent preparation of the manuscript
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© 1984 Springer-Verlag Berlin · Heidelberg
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Danguy, A., Leclercq, G., Heuson, J.C. (1984). Use of Fluorescent Sex-Steroid Conjugates for the Histochemical Detection of Breast Cancer Cell Receptors: A Critique. In: Leclercq, G., Toma, S., Paridaens, R., Heuson, J.C. (eds) Clinical Interest of Steroid Hormone Receptors in Breast Cancer. Recent Results in Cancer Research, vol 91. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82188-2_9
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DOI: https://doi.org/10.1007/978-3-642-82188-2_9
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