Estrogen and Progestin Receptor Analysis in Human Breast Cancer by Isoelectric Focusing in Slabs of Polyacrylamide Gel

  • Ö. Wrange
  • S. Humla
  • I. Ramberg
  • B. Nordenskjöld
  • J.-Å. Gustafsson
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 91)

Abstract

The use of estrogen- and progestin receptor-quantitation as prognostic indicators and for predicting response to endocrine treatment in individual cases of human breast cancer has created a need for convenient methods of receptor analysis. In our experience isoelectric focusing is an efficient method for routine analysis of estrogen receptors [8, 9] and progestin receptors [7] in surgical biopsies of human breast cancer.

Keywords

Glycerol Estrogen Fractionation Sodium Hydroxide Trypsin 

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References

  1. 1.
    Burton K (1968) Determination of DNA concentration with diphenylamide. In: Grossman L, Moldave K (eds) Methods in enzymology, vol 12, partB. Academic, New York, p 163Google Scholar
  2. 2.
    Hjerten S (1962) Molecular sieve chromatography on polyacrylamide gels, prepared according to a simplified method. Arch Biochem Biophys (Suppl) 1: 147–151Google Scholar
  3. 3.
    Okret S, Wränge Ö, Nordenskjöld B, Silfverswärd C, Gustafsson JÅ (1978) Estrogen receptor: assay in human mammary carcinoma with the synthetic estrogen II β-methoxy-17α-ethi- nyl-1,3,5(10) estratriene-3,17β-diol (R2858). Cancer Res 38: 3904–3909PubMedGoogle Scholar
  4. 4.
    Pichon MF, Milgrom E (1977) Characterization and assay of progesterone receptor in human mammary carcinoma. Cancer Res 37: 464–471PubMedGoogle Scholar
  5. 5.
    Powell B, Garola RE, Chamness GC, McGuire WL (1979) Measurement of progesterone receptor in human breast cancer biopsies. Cancer Res 39: 1678–1682PubMedGoogle Scholar
  6. 6.
    Winter A, Ek K, Andersson UB (1977) Analytical electrofocusing in thin layers of polyacrylamide gels. LKB application note 250Google Scholar
  7. 7.
    Wränge Ö, Humla S, Ramberg I, Gustafsson S, Skoog L, Nordenskjöld B, Gustafsson JÄ (1981) Progestin-receptor analysis in human breast cancer cytosol by isoelectric focusing in slabs of Polyacrylamide gel. J Steroid Biochem 14: 141–148PubMedCrossRefGoogle Scholar
  8. 8.
    Wränge Ö, Nordenskjöld B, Gustafsson JÅ (1978) Cytosol estradiol receptor in human mammary carcinoma: An assay based on isoelectric focusing in Polyacrylamide gel. Anal Biochem 85: 461–475PubMedCrossRefGoogle Scholar
  9. 9.
    Wränge Ö, Ramberg I, Gustafsson JÅ, Nordenskjöld B (1978) Estradiol receptor analysis in human breast cancer tissue by isoelectric focusing in Polyacrylamide gel. LKB application note No 313Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1984

Authors and Affiliations

  • Ö. Wrange
    • 1
  • S. Humla
    • 1
  • I. Ramberg
    • 1
  • B. Nordenskjöld
    • 1
  • J.-Å. Gustafsson
    • 1
  1. 1.Department of Medical Nutrition, Department of Tumor Pathology and OncologyKarolinska Institutet, Huddinge University Hospital F 69HuddingeSweden

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