Rubeanic Acid and X-Ray Microanalysis for Demonstrating Metal Ions in Filled Neurons
The staining of neurons with cobalt chloride, either by intracellular injection or infusion through the cut ends of axons, and subsequent precipitation of the black insoluble sulphide within the cells has become a well-established method for determining neuronal morphology (Strausfeld and Obermayer 1976; Altman and Tyrer 1980). Various other metal chlorides can also be used to stain neurons and the filling of different cells with different chlorides in the same preparation would enable the relationships between groups of neurons to be examined if some means of distinguishing between them could be found. Unfortunately the sulphides of the most commonly used metal ions, cobalt and nickel, are similar in appearance, and cannot always be distinguished.
KeywordsNickel Sucrose Sulphide Hydrate Cobalt
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