Can DNA Methylation Regulate Gene Expression?
The DNA from many different animals contains 5-methyl-cytosine (m5C) in addition to the four DNA bases (Wyatt 1951; Vanyushin et al. 1970). m5C is predominantly found in the sequence m5CpG (Grippo et al. 1968), and is known to arise by the direct methylation of cytosine residues in DNA (Sneider and Potter 1969; Burdon and Adams 1969). There is considerable variation in the degree of methylation in DNA from different animals (Waalwijk and Flavell 1978b; Drahovsky et al. 1979; Bird and Taggart 1980; Eden et al. 1981).
KeywordsFormaldehyde Agarose Electrophoresis Sarcoma Cytosine
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