Modified Nucleosides in Body Fluids of Tumor-Bearing Patients

  • F. Salvatore
  • A. Colonna
  • F. Costanzo
  • T. Russo
  • F. Esposito
  • F. Cimino
Part of the Recent Results in Cancer Research/Fortschritte der Krebsforschung/Progrès dans les recherches sur Ie cancer book series (RECENTCANCER, volume 84)


The multifunctional cellular role of tRNA is derived from its molecular structure, which is attained through a complex process of biosynthesis, including covalent modifications of many nucleosides (see Salvatore et al. 1982). In fact tRNA is the nucleic acid species which contains the highest and most varied number of modified nucleosides: among the tRNA species analyzed, more than 40 positions along the primary structure have been found occupied by a modified nucleoside (see Agris 1980). About 50 different types of modified nucleosides have been identified, and in a single tRNA species up to 15 of them can be present. In most cases, three or four are ψ residues and between four and ten are methylated nucleosides (see Dirheimer et al. 1979).


Chick Embryo Fibroblast Histiocytic Lymphoma tRNA Species Modify Nucleoside Morris Hepatoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



ß-aminoisobutyric acid




pseudouridine 5′-phosphate


































N 2-methylguanosine


N 4-acetylcytidine


N 2,N 2-dimethylguanosine










thymidine 5′-phosphate






N 6-threonyladenosine


α-(carboxyamino)-4,9-dihy-dro-4,6-dimethyl-9-oxo-lH-imidazol[l,2-α]-purine-7-butyric acid dimethyl ester




N 2,N 2-dimeth-ylguanine


internal standard


absorbance units full scale


pounds per square inch

A260 unit

quantity of material contained in 1 ml solution which has an absorbance of 1.0 at 260 nm when measured in 1-cm lightpath cell


gas-liquid chromatography


reverse-phase chromatography


high-performance liquid chromatography




snake venom phosphodiesterase


trichloracetic acid




chick embryo fibroblasts


chick embryo fibroblasts transformed by Rous sarcoma virus


avian myeloblastosis virus


cell line derived from African green monkey kidney


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Copyright information

© Springer-Verlag Berlin • Heidelberg 1983

Authors and Affiliations

  • F. Salvatore
    • 1
  • A. Colonna
    • 1
  • F. Costanzo
    • 1
  • T. Russo
    • 1
  • F. Esposito
    • 1
  • F. Cimino
    • 1
  1. 1.Istituto di Chimica Biologica, II Facoltà di Medicina e ChirurgiaUniversità di NapoliNapoliItaly

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