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Alteration of tRNA Modification in Eukaryotes: Causes and Consequences

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Modified Nucleosides and Cancer

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Abstract

In eubacteria the patterns of modified nucleosides of tRNA and their biosynthetic pathways show taxa-specific differences. In general, tRNAs in growing microorganisms are fully modified, but alterations of tRNA modifications can be caused by mutations, unbalanced growth conditions, and inhibitors of post-transeriptional modification, and during sporulation (for summarizing references see Kersten 1981).

This work was supported by Deutsche Forschungsgemeinschaft Grant KE 98/16 and SFB 118. The author thanks Dr. B. Emmerich, München, and Dr. A. Ogilvie, Erlangen, for their cooperation on the work with mouse tumors, Ulrike Lösel and Herbert Brönner for skilful technical assistance, and Sonja Noeth for help with the manuscript

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Abbreviations

U:

uridine

ψ:

pseudouridine

C:

cytidine

N+ :

unidentified uridine derivative

G:

guanidine

Q:

queuosine (7-[(4,5-cis-dihydroxy-2-cyclopenten-l-ylamino)methyl]-7-deazaguanidine)

T or m 5 U :

ribosylthymine. The abbreviations of all other methylated or modified nucleosides are given in the reference cited in the legend of Fig. la. tRNAf Met, formylmethionine tRNA

Poly(Phe):

polyphenylalaline

Poly(U):

polyuridylic acid

Transglycosylase:

tRNA transgly-cosylase (E.C. 2.4.2.-)

A260 unit:

amount of material absorbing at 260 nm that gives an A260 of 1 when soluted in 1 ml (d = 1 cm)

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Universität Erlangen-Nürnberg, Fahrstrasse 17, 8520, Erlangen, Germany

    H. Kersten

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  1. H. Kersten
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Editors and Affiliations

  1. Zentrallaboratorium für Mutagenitätsprüfung, Deutschen Forschungsgemeinschaft, Breisacher Strasse 33, D-7800, Freiburg, Germany

    Gisela Nass

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© 1983 Springer-Verlag Berlin • Heidelberg

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Kersten, H. (1983). Alteration of tRNA Modification in Eukaryotes: Causes and Consequences. In: Nass, G. (eds) Modified Nucleosides and Cancer. Recent Results in Cancer Research/Fortschritte der Krebsforschung/Progrès dans les recherches sur Ie cancer, vol 84. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81947-6_19

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  • DOI: https://doi.org/10.1007/978-3-642-81947-6_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-81949-0

  • Online ISBN: 978-3-642-81947-6

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