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Embryonic Organs in Culture

  • D. M. Kochhar
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 65)

Abstract

It is not my purpose to review the field of experimental embryology that deals with tissue culture of mammalian embryonic organs (Wessells 1967). A number of embryonic organs and tissues are known to undergo morphogenesis and attain functional maturation upon explanation in vitro, and their capabilities have been exploited to a variable degree in investigations on developmental mechanisms and the processes of cell differentiation (Saxén and Kohonen 1969). Some examples are: kidney (Grobstein 1956; Saxén et al. 1968), sex organs (Odor and Blandau 1971; Haffen 1976), pancreas (Sarras et al. 1981; Maylde-Pfenninger and Jamieson 1981; Grobstein 1953), skin (Sugimoto and Endo 1969), tooth (Thesleff 1976; Thesleff et al. 1981), palate (Moriarty et al. 1963; Lahti et al. 1972; Newall and Edwards 1976, 1981; Greene and Pratt 1977), other craniofacial tissues (Johnston and Listgarten 1972; Johnston et al. 1977; Greenberg and Schrier 1977), and limbs (Agnish and Kochhar 1977; Kochhar et al. 1976). Any of these or other developing organs are suitable subjects for the development of a teratologic screening system. This chapter, however, is limited to just one example, i.e., limb bud, that may suffice to bring out feasibility, advantages, and shortcomings of organ culture system in general for short-term teratologic screening purposes.

Keywords

Organ Culture Cytosine Arabinoside Retinyl Palmitate Organ Culture System Apical Ectodermal Ridge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • D. M. Kochhar

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