pO2 and Oxygen Content Measurement in Blood Samples Using Polarographic Oxygen Sensors

  • C. R. Bridges


A major function of blood in most organisms is to supply the tissues with oxygen. The measurement of oxygen partial pressure (\( {p_{{{\rm{O}}_{\rm{2}}}}} \)) and oxygen content (\( {c_{{{\rm{O}}_{\rm{2}}}}} \)) in blood are therefore important criteria through which the state of the gas exchange system of the organism can be assessed.


Oxygen Content Total Oxygen Content Oxygen Dissociation Curve Avian Blood Blood Oxygen Content 
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  1. 1.
    Awad O, Winzler RT (1961) Electrochemical determination of the oxygen content of blood. J Lab Clin Med 58:489–494PubMedGoogle Scholar
  2. 2.
    Baumberger JP (1938) Determination of the oxygen dissociation curve of oxyhaemoglobin by a new method. Am J Physiol 123:10Google Scholar
  3. 3.
    Baumberger JP (1940) The accurate determination of haemoglobin oxyhaemoglobin and carbon monoxide haemoglobin (or myoglobin)by means of a dropping mercury electrode. Am J Physiol 129:308Google Scholar
  4. 4.
    Bishop JM ,Pincock AC, Hollyhock A, Raine J, Cole RB (1966) Factors affecting the measurement of the partial pressure of oxygen in blood using a covered electrode system. Respir Physiol 1:225–237PubMedCrossRefGoogle Scholar
  5. 5.
    Borgström L, Hägerdal M, Lewis L, Pontén U (1974) Polarographic determination of total oxygen content in small blood samples. Scand J Clin Lab Invest 34:375–380PubMedCrossRefGoogle Scholar
  6. 6.
    Bridges CR, Bicudo JEPW, Lykkeboe G (1979) Oxygen content measurement in blood containing haemocyanin. Comp Biochem Physiol 62A:457–462CrossRefGoogle Scholar
  7. 7.
    Clark LC Jr (1956) Monitor and control of blood and tissue oxygen tensions. Trans Am Soc Artif Int Organs 2:41–48Google Scholar
  8. 8.
    Cook SF (1927) The action of potassium cyanide and potassium ferricyanide on certain respiratory pigments. J Gen Physiol 11:339–348CrossRefGoogle Scholar
  9. 9.
    Duvelleroy MA, Buckles RG, Rosenkaimer S, Tung C, Laver MB (1970) An oxyhemoglobin dissociation analyser. J Appl Physiol 28:227–233PubMedGoogle Scholar
  10. 10.
    Eldridge F, Fretwell LK (1965) Change in oxygen tension of shed blood at various temperatures. J Appl Physiol 20:790–792Google Scholar
  11. 11.
    Flenley DC, Millar JS, Rees HA (1967) Accuracy of oxygen and carbon dioxide electrodes. Br Med J 2:349–352PubMedCrossRefGoogle Scholar
  12. 12.
    Gleichmann U, Lübbers DW (1960) Die Messung des Sauerstoffdruckes in Gasen und Flüssigkeiten mit der Pt-Elektrode unter besonderer Berücksichtigung der Messung im Blut. Pfluegers Arch Ges Physiol 271:431–455CrossRefGoogle Scholar
  13. 13.
    Hahn CEW, Davis AH, Albery WJ (1975) Electrochemical improvement of the performance of PO. electrodes. Respir Physiol 25:109–133CrossRefGoogle Scholar
  14. 14.
    Hedley-Whyte J, Radford EP Jr, Laver MB (1965) Nomogram for temperature correction or electrode calibration during PO. measurements. J Appl Physiol 20:785–786PubMedGoogle Scholar
  15. 15.
    Heitmann H, Buckles RG, Laver MB (1967) Blood pO2 measurements: Performance of microelectrodes. Respir Physiol 3:380–395 2PubMedCrossRefGoogle Scholar
  16. 16.
    Kelman G, Nunn JF (1966) Nomograms for correction of blood pO2 , PCO. , pH and base excess for time and temperature. J Appl Physiol 21: 1484–1490PubMedGoogle Scholar
  17. 17.
    Klingenmaier CH, Behar MG, Smith TH (1969) Blood oxygen content measured by oxygen tension after release by carbon monoxide. J Appl Physiol 26: 653–655PubMedGoogle Scholar
  18. 18.
    Laver MB, Murphy AJ, Seifen A, Radford ER Jr (1965) Blood O2 content measurements using the oxygen electrode. J Appl Physiol 20:1063–1069PubMedGoogle Scholar
  19. 19.
    Laver MB, Seifen A (1965) Measurement of blood oxygen tension in anesthesia. Anesthesiology 26:73–101PubMedCrossRefGoogle Scholar
  20. 20.
    Mayers LB, Forster RE (1966) A rapid method for measuring blood oxygen content utilizing the oxygen electrode. J Appl Physiol 21:1393–1396PubMedGoogle Scholar
  21. 21.
    Moran F, Kettel LJ, Cugell DW (1966) Measurement of blood pO2 with the macrocathode electrode. J Appl Physiol 21:725–728 2PubMedGoogle Scholar
  22. 22.
    Neville JR (1960) A simple, rapid polarographic method for blood oxygen content determination. J Appl Physiol 15:717–722PubMedGoogle Scholar
  23. 23.
    Nightingale TE, Boster RA, Fedde MR (1968) Use of the oxygen electrode in recording pO2 in avian blood. J Appl Physiol 25:371–375 2Google Scholar
  24. 24.
    Oeseburg B, Kwant G, Schut JK, Zijlstra WG (1979) Measuring oxygen tension in biological systems by means of Clark-type polarographic electrodes. Proc K Ned Akad Wet Ser C 82:83–90Google Scholar
  25. 25.
    Rawlinson WA (1940) Cristalline hemocyanins: some physical and chemical constants. Austral J Exp Biol Med Sci 18:131–140CrossRefGoogle Scholar
  26. 26.
    Rhodes PG, Moser KM (1966) Sources of error in oxygen tension measurement. J Appl Physiol 21:729–734PubMedGoogle Scholar
  27. 27.
    Roughton FJW, Scholander PF (1945) Micro-gasometric estimation of blood gases I. Oxygen. J Biol Chem 148:541–550Google Scholar
  28. 28.
    Scheid P, Kawashiro T (1975) Metabolic changes in avian blood and their effects on determination of blood gases and pH. Respir Physiol 23:291–300PubMedCrossRefGoogle Scholar
  29. 29.
    Scheid P, Meyer M (1978) Mixing technique for study of oxygenhemoglobin equilibrium: A critical evaluation. J Appl Physiol 45:818–822PubMedGoogle Scholar
  30. 30.
    Severinghaus JW (1968) Measurement of blood gases, pO2 and pCO2Ann NY Acad Sci 148: 115–132 O2 ,CO2 PubMedCrossRefGoogle Scholar
  31. 31.
    Severinghaus JW, Bradley BA (1971) Blood gas electrodes or what the instructions didn’t say. Radiometer, Copenhagen, ST 59, pp 1–64Google Scholar
  32. 32.
    Sigaard-Andersen O (1974) The acid-base status of the blood. Munksgaard, Copenhagen, pp 175–181Google Scholar
  33. 33.
    Solymar M, Rucklidge MA, Prys-Roberts C (1971) A modified approach to the polarographic measurement of blood O2 content. J Appl Physiol 30:272–275PubMedGoogle Scholar
  34. 34.
    Sproule BJ, Miller WF, Cushing IE, Chapman CB (1957) An improved polarographic method of measuring oxygen tension in whole blood. J Appl Physiol 11:365–370PubMedGoogle Scholar
  35. 35.
    Tazawa H (1970) Measurement of O2 content in microliter blood samples. J Appl Physiol 29: 414–416PubMedGoogle Scholar
  36. 36.
    Truchot JP (1978) Variations de la concentration sanguine d’hémocyanine fonctionnelle au couns du cycle d’intermue chez le crabe Carcinus maenas (L.). Arch Zool Exp Gen 119:265 -282Google Scholar
  37. 37.
    Tucker VA (1967) Method for oxygen content and dissociation curves on microliter blood samples. J Appl Physiol 23,410–414PubMedGoogle Scholar
  38. 38.
    Slyke Van DD, Neill JM (1924) The determination of gases in blood and other solutions by vacuum extraction and manometric measurements. I. J Biol Chem 61:523–573Google Scholar
  39. 39.
    Weber RE, Fänge R, Rasmussen KK (1979) Respiratory significance of priapulid hemerythrin. Mar Biol Lett 1:87–97Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • C. R. Bridges
    • 1
  1. 1.Abteilung PhysiologieMax-Planck-Institut für experimentelle MedizinGöttingenGermany

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