Initiation and Establishment of Cell Suspension Cultures of Carrot (Daucus carota)

  • Jakob Reinert
  • Michael Magson Yeoman

Abstract

The culture of plant tissues in an agitated liquid medium eliminates many of the disadvantages ascribed to the culture of tissues on agar. Movement of the tissue in relation to the nutrient medium facilitates gaseous exchange, removes any polarity of the tissue due to gravity, and eliminates nutrient gradients within the medium and at the surface of the cells. The incubation of a friable callus in a liquid nutrient medium agitated on a shaking machine, will eventually give a suspension of cells which are more amenable to experimental manipulation than callus grown on agar. The technique described here is one in which a callus of Daucus carota is suspended in a liquid nutrient medium and is subjected to a series of manipulations to ensure the production of an actively growing cell suspension consisting of single cells and very small aggregates of 2–15 cells.

Keywords

Burner Porosity Agar Nylon 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Henshaw, G.G., lha, K.K., Mehta, A.R., Shake-shaft, D.J., Street H.E.: Studies on the growth in culture of plant cells. I. Growth patterns in batch propagated suspension cultures. J. Exp. Bot. 17 ,362–377 (1966)CrossRefGoogle Scholar
  2. Street H.E.: Cell (Suspension) Culture-Techniques, pp. 61–102, In: Plant Tissue and Cell Culture (Street, H-E., ed.) Botanical Monographs 11, Oxford: Blackwell 1977Google Scholar
  3. Torrey, J.G., Reinert, J.: Suspension culture of higher plant cells in synthetic media. Plant Physiol. 36 ,483–491 (1961)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • Jakob Reinert
    • 1
  • Michael Magson Yeoman
    • 2
  1. 1.Institut für Pflanzenphysiologie und ZellbiologieBerlin 33Federal Republic of Germany
  2. 2.Department of BotanyUniversity of EdinburghEdinburghGreat Britain

Personalised recommendations