The Isolation and Culture of the Primary Seedling Root of Pea (Pisum sativum)
The earliest successful sterile cultures of parts of higher plants were started from excised primary seedling roots. Root tips are attractive candidates because of their meristematic nature, availability, and the property of remaining relatively unperturbed after excision. In these early experiments the excised root tips were grown on a solid substratum from which the excised organs derived both nutrients and mechanical support. The media used were fairly simple in composition and contained, in addition to a natural extract, a carbon source, glucose or sucrose, and a mineral salts mixture consisting of the essential macro and micro elements. Although roots were subcultured with some success it was left to P. R. White to show that detached roots could be grown in aseptic culture for unlimited periods. Subsequently, in a series of investigations roots of pea and tomato have been cultivated in vitro and also used to study basic growth phenomena.
KeywordsBurner Sucrose Rubber Aeration Chlorite
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