Advertisement

The Isolation and Culture of the Primary Seedling Root of Pea (Pisum sativum)

  • Jakob Reinert
  • Michael Magson Yeoman

Abstract

The earliest successful sterile cultures of parts of higher plants were started from excised primary seedling roots. Root tips are attractive candidates because of their meristematic nature, availability, and the property of remaining relatively unperturbed after excision. In these early experiments the excised root tips were grown on a solid substratum from which the excised organs derived both nutrients and mechanical support. The media used were fairly simple in composition and contained, in addition to a natural extract, a carbon source, glucose or sucrose, and a mineral salts mixture consisting of the essential macro and micro elements. Although roots were subcultured with some success it was left to P. R. White to show that detached roots could be grown in aseptic culture for unlimited periods. Subsequently, in a series of investigations roots of pea and tomato have been cultivated in vitro and also used to study basic growth phenomena.

Keywords

Nicotinic Acid Root Culture Pisum Sativum Natural Extract Aseptic Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brown, R., Wightman, F.: The influence of mature tissue on division in the meristem of the root. J. Exp. Bot. 3 ,253–263 (1952)CrossRefGoogle Scholar
  2. Kotte, W.: Wurzelmeristem in Gewebekultur. Ber. Dtsch. Bot. Ges. 40 ,269–272 (1922)Google Scholar
  3. Robbins, W. J.: Cultivation of excised root tips and stem tips under sterile conditions. Bot. Gaz. 73 ,376–390 (1922)CrossRefGoogle Scholar
  4. Street H.E., Butcher, D.N.: Excised root culture. Bot. Rev. 30 ,513–586 (1964)CrossRefGoogle Scholar
  5. White, P. R.: The Cultivation of Plant and Animal Cells, 2nd Ed. New York: Ronald Press 1963Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • Jakob Reinert
    • 1
  • Michael Magson Yeoman
    • 2
  1. 1.Institut für Pflanzenphysiologie und ZellbiologieBerlin 33Federal Republic of Germany
  2. 2.Department of BotanyUniversity of EdinburghEdinburghGreat Britain

Personalised recommendations