Summary
Using fluorescence microscopy a higher content of lectin binding sites was observed in the mucosubstances of transitional mucosa adjacent to colonic carcinomas than in normal mucosa. The lectin binding pattern of adenomas was similar to that of the transitional mucosa. However, in proliferating parts and areas with cell atypia the amount of lectin receptors was generally reduced. In colonic carcinomas the occurrence of lectin receptors was correlated with the morphological degree of differentiation: well differentiated carcinomas showed more lectin binding sites than undifferentiated ones. Often a concomitant occurrence of FYTC-Ricinus communis agglutinin with carcinoembryonic antigen was found; the latter is known to be a glycoprotein with a high carbohydrate content. Of special interest was the peanut agglutinin receptor that was only demonstrable in transitional mucosa and carcinomas but was absent in normal mucosa of the colon. The importance of this receptor is discussed with respect to the occurrence of blood group antigens and immunotherapeutical considerations in colonic carcinomas.
The histopathology and classification of tumours of the colon is well established (survey by Morson and Sobin [17]; also [6]). However, in future the morphological differentiation alone may not be adequate for the characterisation of these tumours. With respect to new therapeutical considerations, therefore, further investigations are needed that give more detailed information about the metabolism of tumour cells. This development in oncology requires a more sensitive microscopical diagnosis with histochemical methods that allow the demonstration of distinct cell components. In this context the analysis of the mucins in the epithelial cells of the colon seems to be of great importance [3, 4, 7, 10, 12, 13, 16, 18]. There is still much, to be learned about the role of the different monosaccharides to determine the significance of mucins in the physiology and pathology of the bowel.
In this study different carbohydrate structures have been investigated by fluorescence microscopy in normal, hyperplastic, adenomatous, and carcinomatous colonic mucosa. Lectins that are well known for their carbohydrate specificity (survey by Sharon and Lis [21]), were used for visualisation of these sugar components; the lectins were labelled by conjugation with fluorescein isothiocyanate (FITC). Additionally, in our histochemical investigations the occurrence of lectin receptors was compared with the localisation of carcinoembryonic antigen (CEA), a tumour marker that possesses a high content of carbohydrates [15].
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References
Burtin P, Calmettes C, Fondaneche MC (1979) CEA and non-specific cross reacting antigen ( NCA) in medullary carcinomas of the thyroid. Int J Cancer 23: 741
Denk H, Tappheimer G, Eckerstorfer R, Holzner JH (1972) Carcinoembryonic antigen ( CEA) in gastrointestinal and extragastrointestinal tumors and its relationship to tumor-cell differentiation. Int J Cancer 10: 262
Essner E, Schreiber J, Griewski RA (1978) Localisation of carbohydrate components in rat colon with fluorescinated lectins. J Histochem Cytochem 26: 452
Filipe MJ (1979) Mucins in the human gastrointestinal epithelium: a review. Invest Cell Pathol 2: 195
Fox RA (1979) Membrane glycoproteins shed in defence of the cells of the gastrointestinal tract. Med Hypotheses 5: 669
Gibbs NM (1979) Histological typing of intestinal tumours (WHO) with particular reference to epithelial tumours of the colon. In: Grundmann E (ed) Colon cancer. Cancer campaign, vol 2. Fischer, Stuttgart New York, p 113
Gorman TA, Lamont JT (1978) Glycoprotein synthesis and secretion in human colon cancers and normal colonic mucosa. Cancer Res 38: 2784
Huitric E, Laumonier R, Burtin P, von Kleist S, Chavanel G (1976) An optical and ultrastructural study of the localisation of carcinoembryonic antigen ( CEA) in normal and cancerous human rectocolonic mucosa. Lab Invest 34: 97
Isaacson P, Le Vann HP (1976) The demonstration of carcinoembryonic antigen in colorectal carcinoma and colonic polyps using an immunoperoxidase technique. Cancer 38: 1348
Kim YS, Isaacs R, Perdomo JM (1974) Alterations of membrane glycopeptides in human colonic adenocarcinoma. Proc Natl Acad Sei USA 71: 4869
Klein PJ, Newman RA, Müller P, Uhlenbruck G, Schaefer HE, Lennartz KJ, Fischer R (1978) Histochemical methods for the demonstration of Thomsen-Friedenreich antigen in cell suspensions and tissue sections. Klin Wochenschr 56: 761
Korhonen LK, Mäkelä V, Lilius G (1971) Carbohydrate-rich compounds in the colonic mucosa of man. II. Histochemical characteristics of colonic adenocarcinomas. Cancer 27: 128
La Mont JT, Ventola A (1978) Galactosyltransferase in fetal, neonatal, and adult colon: relationship to differentiation. Am J Physiol 235: 213
Lotan R, Skutelsky E, Damon D, Sharon N (1975) The purification, composition and specificity of the anti-T lectin from peanut ( Arachis hypogaea ). J Biol Chem 250: 8518
MacSween JM, Fox RA (1975) Carcinoembryonic antigen: characterisation of binding with insoluble lectins. Br J Cancer 31: 288
Mäkelä V, Korhanen LK, Lilius G (1971) Carbohydraterich compounds in the colonic mucosa of man. I. Histochemical characteristics of normal and adenomatous colonic mucosa. Cancer 27: 120
Morson BC, Sobin LH (1976) Histological typing of intestinal tumours. World Health Organisation, Geneva. (International histological classification of tumours no. 15 )
Reid PE, Culling CFA, Dunn WL, Ramey CW, Magil AB, Clay MG (1980) Differences between O-acetylated sialic acids of the epithelial mucins of human colonic tumors and normal controls. J Histochem Cytochem 28: 217
Rogalsky VY (1975) Variations in carcinoembryonic antigen localisation in tumours of the colon. J Natl Cancer Inst 54: 1061
Sedlacek HH, Seiler FR (1978) Immunotherapy of neoplastic diseases with neuraminidase: contradictions, new aspects, and revised concepts. Cancer Immunol Immunother 5: 153
Sharon N, Lis H (1972) Lectins: Cell agglutinating and sugar specific proteins. Science 177: 949
Springer GF, Desai PR, Banatwala J (1975) Blood group MN antigens and precursors in normal and malignant human breast glandular tissue. J Natl Cancer Inst 54: 335
Uhlenbruck G, Pardoe GI, Bird GW (1969) On the specificity of lectins with a broad agglutination spectrum. II. Studies on the nature of the T-antigen and the specific receptors for the lectin of Arachis hypogaea (ground nut). Z Immunitaetsforsch 138: 423
Uhlenbruck G, Wintzer G, Kania J, Koch O (1980) Immunologische und klinischdia- gnostische Studien an tumor-assoziierten Antigenen. Westdeutscher Verlag, Opladen. ( Forschungsberichte des Landes Nordrhein-Westfalen, Nr. 2903 )
Wagener C, Csaszar H, Totovic V, Breuer H (1978) A highly sensitive method for the demonstration of carcinoembryonic antigen in normal and neoplastic colonic tissue. Histochemistry 58: 1
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Klein, P.J., Osmers, R., Vierbuchen, M., Ortmann, M., Kania, J., Uhlenbruck, G. (1981). The Importance of Lectin Binding Sites and Carcinoembryonic Antigen with Regard to Normal, Hyperplastic, Adenomatous, and Carcinomatous Colonic Mucosa. In: Klein, H.O. (eds) Chemotherapy and Radiotherapy of Gastrointestinal Tumors. Recent Results in Cancer Research, vol 79. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81681-9_1
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DOI: https://doi.org/10.1007/978-3-642-81681-9_1
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