Hair Research pp 215-222 | Cite as

The Metabolism of Androgens in Skin

  • V. A. Randall
  • F. J. Ebling

Abstract

Testosterone, previously considered to be the major androgen, 1 now appears to require metabolism by its target cell before it can be used (King and Mainwaring, 1974; Mainwaring, 1976). The simplified theory of androgen action (Fig. 1) considers that testosterone is carried around the body in the blood — both as free testosterone and bound to the plasma proteins — and enters the target cell by a method which is not completely understood but which may involve a carrier mechanism (Giorgi et al., 1974; Giorgi, 1976). In the cytoplasm testosterone is metabolized by the enzyme 5α-reductase to 5α-dihydrotestosterone and is then bound to a cytoplasmic receptor protein. The dihydrotestosterone-receptor complex changes in configuration before entering the nucleus, where it is bound to the chromatin and thus stimulates an ordered temporal sequence of biochemical events which bring about cellular responses to androgens. The mechanism of release and removal of the hormone-receptor complex are as yet unknown.

Keywords

Testosterone Androgen Diol Acne Dermatol 

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References

  1. Adachi K, Kano M (1972) The role of receptor proteins in controlling androgen action in the sebaceous glands of hamsters. Steroids 19:567–574PubMedCrossRefGoogle Scholar
  2. Baulieu EE, Lasnitzki I, Robel P (1968) Metabolism of testosterone and action of metabolites on prostate glands grown in organ culture. Nature 219:1155–1156PubMedCrossRefGoogle Scholar
  3. Bingham KD, Shaw DA (1973) The metabolism of testosterone by human male scalp skin. J Endocrinol 57:111–121PubMedCrossRefGoogle Scholar
  4. Booth WD (1972) The occurrence of testosterone and 5α-dihydrotestosterone in the sub-maxillary salivary gland of the boar. J Endocrinol 55:119–125PubMedCrossRefGoogle Scholar
  5. Collier ME, Griffin JE, Wilson JD (1978) Intranuclear binding of 3H dihydrotestosterone by cultured human fibroblasts. Endocrinology 103:1499–1505PubMedCrossRefGoogle Scholar
  6. Ebling FJ (1974) Hormonal control and methods of measuring sebaceous gland activity. J Invest Dermatol 62:161–171PubMedCrossRefGoogle Scholar
  7. Ebling FJ, Ebling E, McCaffery V, Skinner J (1971) The response of the sebaceous glands of the hypophysectomized-castrated male rat to 5α-dihydrotestosterone, androstenedione, de-hydroepiandrosterone and androsterone. J Endocrinol 51:181–190PubMedCrossRefGoogle Scholar
  8. Ebling FJ, Ebling E, McCaffery V, Skinner J (1973) The responses of the sebaceous glands of the hypophysectomized-castrated male rat to 5α-androstanedione and 5α-androstane-3α, 17β-diol. J Invest Dermatol 60:183–187PubMedCrossRefGoogle Scholar
  9. Eppenberger U, Hsia SL (1972) Binding of steroid hormones by the 105,000Xg supernatant fraction from homogenates of rat skin and variations during the hair cycle. J Biol Chem 247:5463–5469PubMedGoogle Scholar
  10. Ewing JA, Rouse BA (1978) Hirsutism, race and testosterone levels: comparison of East Asians and Euroamericans. Hum Biol 50:209–215PubMedGoogle Scholar
  11. Fazekas AG, Lanthier A (1971) Metabolism of androgens by isolated human beard hair follicles. Steroids 18:367–379PubMedCrossRefGoogle Scholar
  12. Giorgi EP (1976) Studies on androgen transport into canine prostate in vitro. J Endocrinol 68:109–119PubMedCrossRefGoogle Scholar
  13. Giorgi EP, Moses TF, Grant JK, Scott R, Sinclair J (1974) In vitro studies on the regulation of androgen-tissue relationships in canine normal and human hyperplastic prostate. Mol Cell Endocrinol 1:271–284CrossRefGoogle Scholar
  14. Gomez EC, Hsia SL (1968) In vitro metabolism of testosterone-4-14C and ∆4-androstene-3, 17-dione-4-14C in human skin. Biochemistry 7:24–32PubMedCrossRefGoogle Scholar
  15. Griffin JE, Punyashthiti J, Wilson JD (1976) Dihydrotestosterone binding by cultured human fibroblasts; comparison of cell from control subjects and from patients with hereditary male pseudohermaphroditism due to androgen resistance. J Clin Invest 57:1342–1351PubMedCrossRefGoogle Scholar
  16. Guarrera M, Cardo P, Moretti G, Rampini E, Divano C (1976) Studies on rat hair cultures. IV. The effects of testosterone and dihydrotestosterone. Arch Dermatol Res 256:275–281PubMedCrossRefGoogle Scholar
  17. Hay JB (1977) A study of the in vitro metabolism of androgens by human scalp and pubic skin. Br J Dermatol 97:237–246PubMedCrossRefGoogle Scholar
  18. Hay JB, Hodgins MB (1973) Metabolism of androgens in vitro by human facial and axillary skin. J Endocrinol 59:475–486PubMedCrossRefGoogle Scholar
  19. Hay JB, Hodgins MB (1978) Distribution of androgen metabolising enzymes in isolated tissues of human forehead and axillary skin. J Endocrinol 79:29–39PubMedCrossRefGoogle Scholar
  20. Jung I, Baulieu EE (1972) Testosterone cytosol ‘receptor’ in the rat levator ani muscle. Nature New Biol 237:24–26PubMedGoogle Scholar
  21. Keenan BS, Meyer WJ, Hadjian AJ, Migeon CJ (1975) Androgen receptor in human skin fibroblasts. Characterization of a specific 17β/hydroxy-5α-androstan-3-one-protein complex in cell sonicates and nuclei. Steroids 25:535–552PubMedCrossRefGoogle Scholar
  22. King RJB, Mainwaring WIP (1974) Steroid-Cell interactions. Butterworths, LondonGoogle Scholar
  23. Mainwaring WIP (1976) The mechanism of action of androgens. Monographs on endocrinology, Vol 10. Springer, Berlin Heidelberg New YorkGoogle Scholar
  24. Mangan FR, Pegg AE, Mainwäring WIP (1973) A reappraisal of the effects of adenosine 3:5-cyclic monophasphate on the function and morphology of the rat prostate gland. Biochem J 134:129–142PubMedGoogle Scholar
  25. Northcutt RC, Island DP, Liddle GW (1969) An explanation for the target organ unresponsiveness to testosterone in the testicular feminization syndrome. J Clin Endocrinol 29:422–425CrossRefGoogle Scholar
  26. Ohno S, Dofuku R, Tettenborn U (1971) More about X-linked testicular feminization of the mouse as a noninducible (is) mutation of a regulatory locus: 5α-andostan-3α, 17-diol as the true inducer of kidney alcohol dehydrogenase and B-glucoronidase. Clin Genet 2:128–140PubMedCrossRefGoogle Scholar
  27. Röbel P, Lasnitzki I, Baulieu EE (1971) Hormone metabolism and action of testosterone and metabolites in prostate organ culture. Biochimie 53:81–96CrossRefGoogle Scholar
  28. Sansone-Bazzano G, Reisner RM, Bazzano G (1972) Conversion of testosterone-1, 2-3H to an-drostenedione 3H in the isolated hair follicles of man. J Clin Endocrinol Metab 34:512–515PubMedCrossRefGoogle Scholar
  29. Schweikert HU, Milewich L, Wilson JD (1975) Aromatization of androstenedione by isolated human hairs. J Clin Endocrinol Metab 40:413–417PubMedCrossRefGoogle Scholar
  30. Schweikert HU, Milewich L, Wilson JD (1976) Aromatization of androstenedione by cultured human fibroblasts. J Clin Endocrinol Metab 43:785–795PubMedCrossRefGoogle Scholar
  31. Schweikert H, Wilson JD (1974) Regulation of human hair growth by steroid hormones. I. Testosterone metabolism in isolated hair. J Clin Endocrinol Metab 38:811–819PubMedCrossRefGoogle Scholar
  32. Singhai RL, Parulekar MR, Vijayuargiya R, Robinson GA (1971) Metabolic control mechanisms in mammalian systems. Biochem J 125:329–342Google Scholar
  33. Takayasu S, Adachi K (1972) The conversion of testosterone to 17β-hydroxy-5α-androstan-3-one (dihydrotestosterone) by human hair follicles. J Clin Endocrinol Metab 34:1098–1101PubMedCrossRefGoogle Scholar
  34. Takayasu S, Adachi K (1975) The intranuclear binding of 17β-hydroxy-5α-androstan-3-one and testosterone by hamster sebaceous glands. Endocrinology 96:525–529PubMedCrossRefGoogle Scholar
  35. Tremblay RR, Foley TD, Corvol P, Park I, Kowarski A, Blizzard R M, Jones MW, Migeon CJ (1977) Plasma conception of testosterone, ihydrotestosterone, testosterone-oestradiol binding globulin, and pituitary gonadotrophins in the syndrome of male pseudo-hermaphroditism with testicular feminization. Acta Endocrinol 70:331–341Google Scholar
  36. Weiner AL, Ofner P, Sweeney EA (1970) Metabolism of testosterone-4-14C by the canine submaxillary gland in vivo. Endocrinology 87:406–409PubMedCrossRefGoogle Scholar
  37. Wilson JD (1975) Dihydrotestosterone formation in cultured human fibroblasts: Comparison of cells from normal subjects with familial incomplete male pseudohermaphroditism type 2. J Biol Chem 250:3498–3504PubMedGoogle Scholar
  38. Wilson JD, Walker JD (1969) The conversion of testosterone to 5α-androstan-17β-ol-3-one (dihydrotestosterone) by skin slices of man. J Clin Invest 48:371–379PubMedCrossRefGoogle Scholar
  39. Wotiz HH, Mescon H, Doppel H, Lemon HM (1956) The in vitro metabolism of testosterone by human skin. J Invest Dermatol 26:113–120PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • V. A. Randall
    • 1
  • F. J. Ebling
    • 1
  1. 1.Department of ZoologyThe University of SheffieldSheffieldGreat Britain

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