Cellular Composition of Post-haemorrhagic Opacities in the Human Vitreous

  • J. V. Forrester
  • W. R. Lee
Conference paper
Part of the Current Research in Ophthalmic Electron Microscopy book series (CR OPHTHALMIC, volume 4)

Abstract

The morphology of vitreous membranes from enucleated human eyes containing vitreous haemorrhage was studied by electron microscopy. Three types of membrane are described, based on their cellular composition: haematogenous, fibroblastic and neovascular. Simple vitreous haemorrhages usually failed to stimulate a fibroblastic cellular response, whereas vitreous blood clots in eyes with penetrating injuries were frequently invaded by choroidal and/or scleral fibroblasts. Fibroblast-like cells were also found in neovascular membranes, but not as a major cellular component. They had the appearances of astrocytes, suggesting an origin from the retina or optic disc in association with the intravitreal new vessel growth.

These data suggest that two factors are necessary for intravitreal fibrosis: an adequate port of entry for cellular invasion and a suitable substratum on which migrating cells can crawl.

Keywords

Migration Retina Fibril Glaucoma Glutaraldehyde 

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References

  1. Balazs EA, Darzynkiewicz Z (1973) The effect of hyaluronic acid on fibroblasts, mononuclear phagocytes and lymphocytes. In “The Biology of the Fibroblast”. Eds, Kulonen E and Pikkarain-en J Pp 237–254, Academic Press, New York and LondonGoogle Scholar
  2. Cleary PE, Ryan SJ (1979) Experimental posterior penetrating eye injury to the rabbit. II Histology of wound, vitreous and retina. Brit J Ophthal 63: 312–321PubMedCrossRefGoogle Scholar
  3. Cleary PE, Ryan SJ (1979) Histology of wound, vitreous and retina in experimental posterior penetrating eye injury in the rhesus monkey. Amer J Ophthal 88: 221–231PubMedGoogle Scholar
  4. Cliff WJ (1963) Observations on healing tissue: a combined light and electron microscopic investigation. Phil Trans B 246: 305–325CrossRefGoogle Scholar
  5. Constable IJ (1975) The pathology of vitreous membranes. Trans Ophthal Soc UK 95: 382–386PubMedGoogle Scholar
  6. Duke-Elder S, Dobree JH (1977) Diseases of the retina. In: “System of Ophthalmology” Volume 10, p 150. Henry Kimpton LondonGoogle Scholar
  7. Faulborn J, Topping TM (1978) Proliferation irr the vitreous cavity after perforating injuries. A histopathological study. A von Graefes Arch klin exp Opthal 205: 157–177CrossRefGoogle Scholar
  8. Foos RJ (1977) Vitreoretinal juncture over retinal vessels. A von Graefes Arch Klin exp Ophthal 204:233–234Google Scholar
  9. Forrester JV, Lee WR, Williamson J (1978) The pathology of vitreous haemorrhage. I Gross and histological appearances. Arch Ophthal 96: 703–710PubMedCrossRefGoogle Scholar
  10. Forrester JV, Grierson I, Lee WR (1979) The pathology of vitreous haemorrhage II. Ultrastructure Arch Ophthal 97: 2368–2374Google Scholar
  11. Forrester JV, Grierson I, Lee WR (1980) Vitreous membrane formation after experimental vitreous haemorrhage. A von Graefes Arch klin exp Ophthal 212: 227–242CrossRefGoogle Scholar
  12. Forrester JV, Lackie JM Inhibition of neutrophil adhesion by hyaluronic acid (in press) J. Cell SciGoogle Scholar
  13. Forrester JV, Wilkinson PC (in press) Inhibition of neutrophil locomotion by hyaluronic acid. J Cell SciGoogle Scholar
  14. Frielich, DB, Lee PF, Freeman HM (1966) Experimental retinal detachment. Arch Ophthal 20:432–436CrossRefGoogle Scholar
  15. Hogan MJ, Zimmerman LE (1960) Opthalmic Pathology p 650 WB Saunders Co, PhiladelphiaGoogle Scholar
  16. Hogan MJ, Alvarado JA, Weddell JE (1971) Histology of the Human Eye p 613 WB Saunders Co PhiladelphiaGoogle Scholar
  17. Klien BA (1938) Retinitis proliferans. Clinical and histological studies. Arch Ophthal 20: 427–436CrossRefGoogle Scholar
  18. Michaelson IC, Kraus J (1943) War injuries of the eye. Brit J Ophthal 27: 449–461PubMedCrossRefGoogle Scholar
  19. Oguchi L (1913) Über die Wirkung von Blutinjektionen in den Glaskörper nebst Bemerkungen über die sog. Retinitis proliferans. A von Graefes Arch klin exp Ophthal. 84: 446–520Google Scholar
  20. Rentsch FJ (1977) The ultrastructure of preretinal macular fibrosis. A von Graefes Arch klin exp Ophthal 203: 321–337CrossRefGoogle Scholar
  21. Smith RS, van Heuven WAJ, Streeten B (1976) Vitreous membranes: a light and electron microscopical study. Arch Ophthal 94: 1556–1560PubMedCrossRefGoogle Scholar
  22. Treacher-Collins E (1929) Formative fibrous tissue reaction. Trans Ophthal Soc UK 49: 166–203Google Scholar
  23. Underhill C, Dorfman A (1978) The role of hyaluronic acid in intercellular adhesion of cultured mouse cells. Exp Cell Res 117: 155–164PubMedCrossRefGoogle Scholar
  24. Yashamita T, Cibis P (1961) Experimental retinitis proliferans in the rabbit. Arch Ophthal 65: 49–58CrossRefGoogle Scholar
  25. Zinn KM, Constable IJ, Schepens CL (1977) The fine structure of human vitreous membranes. In: “Vitreous surgery and advances in fundus diagnosis and treatment”. Eds Freeman HM, Hirose T, and Schepens CL pp 39–49, New YorkGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1981

Authors and Affiliations

  • J. V. Forrester
    • 1
  • W. R. Lee
    • 1
  1. 1.Tennent Institute of OphthalmologyGlasgowUK

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