Advertisement

Endothelial Surface Charge: Blood-Brain Barrier Opening to Horseradish Peroxidase Induced by the Polycation Protamin Sulfate

  • Z. Nagy
  • H. Peters
  • I. Hüttner
Part of the Acta Neuropathologica Supplementum book series (NEUROPATHOLOGIC, volume 7)

Summary

Perfusion of rat brain with the polycation protamin sulfate caused a decrease in the stainability of luminal surface of cerebral endothelium to positively charged colloidal iron. This change reflecting a decrease in the anionic sites of the endothelial plasma membrane was accompanied by opening of the blood-brain barrier to horseradish peroxidase. The polyanion heparin reversed colloidal iron binding but failed to prevent permeability alteration of the cerebral endothelium.

Key words

blood-brain barrier polycation protein endothel 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    De Bruyn PPH, Michelson S, Becker RP (1978) Nonrandom distribution of sialic acid over the cell surface of bristle-coated endocytic vesicles of the sinusoidal endothelium cells. J Cell Biol 78:379–389PubMedCrossRefGoogle Scholar
  2. 2.
    Nagy Z, Pappius HM, Mathieson G, Hüttner I (1979) Opening of tight junctions in cerebral endothelium. I Effect of hyperosmolar mannitol infused through the internal carotide artery. J Comp Neurol 185:569–578PubMedCrossRefGoogle Scholar
  3. 3.
    Pelikan P, Gimbrone Jr MA, Cotran RS (1979) Distribution and movement of anionic cell surface sites in cultured human vascular endothelial cells. Atherosclerosis 32:69–80PubMedCrossRefGoogle Scholar
  4. 4.
    Reese TS, Karnovsky MH (1967) Fine structural localisation of a blood-brain barrier to exogenous peroxidase. J Cell Biol 34:207–217PubMedCrossRefGoogle Scholar
  5. 5.
    Rinehart JF, Abul-Haj SK (1951) An improved method for histologic demonstration of acid mucopolysaccharides in tissues. Arch Pathol 52:189–194Google Scholar
  6. 6.
    Seiler MW, Venkatachalam MA, Cotran RS (1975) Glomerular epithelium: structural alteration induced by polycations. Science 189: 390–393PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Z. Nagy
    • 1
  • H. Peters
    • 2
  • I. Hüttner
    • 2
  1. 1.Department of PsychiatrySemmelweis Medical UniversityBudapestHungary
  2. 2.Department of PathologyMcGill University MontrealCanada

Personalised recommendations