Gramicidin S-Synthetase: On the Structure of a Polyenzyme Template in Polypeptide Synthesis

  • H. Kleinkauf
  • H. Koischwitz
Part of the Molecular Biology, Biochemistry and Biophysics book series (MOLECULAR, volume 32)


In the pre-RNA history of protein biosynthesis two models of protein formation were proposed (Campbell and Work 1953): Enzymic transpeptidation and the “template theory”.


Limited Proteolysis Amino Acid Analog Peptide Bond Formation Bacillus Brevis Nucleic Acid Template 
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  1. Altmann M, Koischwitz H, Salnikow S, Kleinkauf H (1978) (3,3′-Leu)-Gramicidin S formation by gramicidin S-synthetase. FEBS Lett 93: 247–250PubMedCrossRefGoogle Scholar
  2. Altmann M, Koischwitz H, Kittelberger R (1979) Isolation of partially active structures of the multi-enzyme gramicidin S-synthetase. 11th Inter Congr Biochem Toronto 1979. Abstract 04–5-S 107. Nat Res Council Canada, Can Biochem Soc, Int Union Biochem, OttawaGoogle Scholar
  3. Campbell PN, Work TS (1953) Biosynthesis of proteins. Nature 171: 997–1001PubMedCrossRefGoogle Scholar
  4. Crick FHC (1958) On protein synthesis. In: The biological replication of macromolecules, pp 138 – 163. Symp Soc Exp Biol No 12. University Press, CambridgeGoogle Scholar
  5. Dounce AL (1952) Duplicating mechanism for peptide chain and nucleic acid synthesis. Enzymologia 15: 251–258PubMedGoogle Scholar
  6. Gilhuus-Mode CC, Kristensen T, Bredesen JE, Zimmer T-L, Laland SG (1970) The presence and possible role of phosphopantothenic acid in gramicidin S-synthetase. FEBS Lett 7: 287–290CrossRefGoogle Scholar
  7. Kambe M, Imae Y, Kurahashi K (1974) Biochemical studies on gramicidin S nonproducing mutants of Bacillus brevis ATC 9999. J Biochem 75: 481–493PubMedGoogle Scholar
  8. Kanda M, Hori K, Kurotsu T, Miura S, Nozoe A, Saito Y (1978) Studies on gramicidin S-synthetase. Purification and properties of the light enzyme obtained from some mutants of Bacillus brevis. J Biochem 84: 435–441PubMedGoogle Scholar
  9. Kleinkauf H (1979) Antibiotic polypeptides-biosynthesis on multifunctional protein templates. Planta Med 35: 1–18PubMedCrossRefGoogle Scholar
  10. Kleinkauf H, Koischwitz H (1974) Gramicidin S-synthetase: Active form on the multienzyme complex is undissociable by sodium dodecylsulfate. In: Richter D (ed) Lipmann Symposium: Energy, biosynthesis and regulation in molecular biology. de Gruyter, Berlin New YorkGoogle Scholar
  11. Kleinkauf H, Koischwitz H (1978) Peptide bond formation in nonribosomal systems. In: Hahn FE (ed) Progress in molecular and subcellular biology, vol VI. Springer, Berlin Heidelberg New YorkGoogle Scholar
  12. Kleinkauf H, Koischwitz H (1980a) Gramicidin S-synthetase. In: Bisswanger H, Schmincke-Ott E, (eds) Multifunctional proteins, chap 8. Academic Press, New YorkGoogle Scholar
  13. Kleinkauf H, Koischwitz H (1980b) Gramicidin S-synthetase 1979. In: Proceedings leopoldina symposium. Nover L, Lynen F, Mothes K (eds) Cell compartmentation and metabolic channeling, pp 147–158. Gustav Fischer, Jena, Elsevier, Amsterdam New York OxfordGoogle Scholar
  14. Kleinkauf H, Roskoski R Jr, Lipmann F (1971) Pantetheine-linked peptide intermediates in gramicidin S and tyrocidine biosynthesis. Proc Natl Acad Sci USA 68: 2069–2072PubMedCrossRefGoogle Scholar
  15. Koischwitz H (1975) Non-ribosomal polypeptide formation: A model for peptide sequence fixation on protein templates. 1oth FEBS Meet Paris 1975, abstract 860. Societe de Chimie Biologique, ParisGoogle Scholar
  16. Koischwitz H (1979) Zur Struktur der Proteinmatrize von Gramicidin S. Hoppe-Seyler’s Z Physiol Chem 360: 307Google Scholar
  17. Lee SG, Lipmann F (1977) Isolation of amino acid activating subunit-pantetheine protein complexes: Their role in chain elongation in tyrocidine synthesis. Proc Natl Acad Sci USA 74: 2343–2347PubMedCrossRefGoogle Scholar
  18. Lipmann F (1954) On the mechanism of some ATP-linked reactions and certain aspects of protein synthesis. In: McElroy WD, Glass B (eds) The mechanism of enzyme action, pp 599–604. Hopkins, BaltimoreGoogle Scholar
  19. Lipmann F (1956) Attempts at the formulation of some basic biochemical questions. In: Green DE (ed) Currents in biochemical research, pp 241–250. Interscience, New YorkGoogle Scholar
  20. Lipmann F (1968) The relation between the direction and the mechanism of polymerization. Essays Biochem 4: 1–23PubMedGoogle Scholar
  21. Lipmann F (1971) Attempts to map a process evolution of peptide biosynthesis. Science 173: 875–884PubMedCrossRefGoogle Scholar
  22. Pass L, Zimmer T-L, Laland SG (1974) On the use of affinity chromatography in demonstrating the transfer of thioester-bound D-phenylalanine from the light enzyme of gramicidin S-synthetase to an acceptor site for this amino acid on the heavy enzyme. Eur J Biochem 47: 607–611PubMedCrossRefGoogle Scholar
  23. Spiegelmann S (1954) Nucleic acids and the synthesis of proteins. In: McElroy WD, Glass B (eds) The chemical basis of heredity, pp 232:249. Hopkins, BaltimoreGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1980

Authors and Affiliations

  • H. Kleinkauf
    • 1
  • H. Koischwitz
    • 1
  1. 1.Max-Volmer-Institut für Physikalische Chemie und MolekularbiologieTechnische Universität BerlinBerlin 10Germany

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