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Chemical Recognition in Biology pp 165–182Cite as

The Genes for and Regulation of the Enzyme Activities of two Multifunctional Proteins Required for the De Novo Pathway for UMP Biosynthesis in Mammals

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Part of the book series: Molecular Biology, Biochemistry and Biophysics ((MOLECULAR,volume 32))

Abstract

The biosynthesis of the first pyrimidine nucleotide, UMP, universally requires six enzymes (Reichard 1959): a glutamine-utilizing carbamyl-P synthetase (Levenberg 1962); aspartate transcarbamylase; dihydroorotase; a dihydroorotate dehydrogenase (Taylor et al. 1966) that couples with the respiratory chain (and not with NAD); orotate phos-phoribosyltransferase and orotidylate decarboxylase (Fig. 1).

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References

  • Allen CM Jr, Jones ME (1964) Decomposition of carbamyl phosphate in aqueous solutions. Biochemistry 3: 1238–1247

    Article  PubMed  CAS  Google Scholar 

  • Appel SH (1968) Purification and kinetic properties of brain orotidine 5′-phosphate decarboxylase. J Biol Chem 243: 3924–3929

    PubMed  CAS  Google Scholar 

  • Becker MA, Argubright KF, Fox RM, Seegmiller JE (1974) Oxipurinol-associated inhibition of pyrimidine synthesis in human lymphoblasts. Mol Pharmacol 10: 657–668

    CAS  Google Scholar 

  • Bresnick E, Blatchford K (1964) Studies on dihydroorotase activity in preparations from Novikoff ascites hepatoma cells. Arch Biochem Biophys 104: 381–386

    Article  PubMed  CAS  Google Scholar 

  • Bresnick E, Mayfield ED Jr, Mosse H (1968) Increased activity of enzymes for de novo pyrimidine biosynthesis after orotic acid administration. Mol Pharmacol 4: 173–180

    PubMed  CAS  Google Scholar 

  • Brown GK, O’Sullivan WJ (1977) Subunit structure of orotate phosphoribosyltransferase — orotidy-lated decarboxylase complex from human erythrocytes. Biochemistry 16: 3235–3242

    Article  PubMed  CAS  Google Scholar 

  • Brown GK, Fox RM, O’Sullivan WJ (1975) Interconversion of different molecular weight forms of human erythrocyte orotidylate decarboxylase. J Biol Chem 250: 7352–7358

    PubMed  CAS  Google Scholar 

  • Buttoo AS, Israel MCG, Wilkinson JF (1965) Hypocholesterolaemia and orotic aciduria during treatment with 6-azauridine. Brit Med J 1: 552–554

    Article  PubMed  CAS  Google Scholar 

  • Campbell MT, Gallagher ND, O’Sullivan WJ (1977) Multiple molecular forms of orotidylate decarboxylase from human liver. Biochem Med 17: 128–140

    Article  PubMed  CAS  Google Scholar 

  • Cardoso SS, Calabresi P, Handschumacher RE (1961) Alterations in human pyrimidine metabolism as a result of therapy with 6-azauridine. Cancer Res 21: 1551–1556

    PubMed  CAS  Google Scholar 

  • Chen J-J, Jones ME (1976) The cellular location of dihydroorotase dehydrogenase: Relation to de novo biosynthesis of pyrimidine. Arch Biochem Biophys 176: 82–90

    Article  PubMed  CAS  Google Scholar 

  • Chen J-J, Jones ME (1978) The regulation of de novo pyrimidine biosynthesis by cellular phospho-ribosyl pyrophosphate levels in cultured Ehrlich ascites cells. In: Srere PA, Estabrook RW (eds) Microenvironments and metabolic compartmentation, pp 211–226. Academic Press, New York San Francisco London

    Google Scholar 

  • Chen J-J, Jones ME (1979a) Effect of 5-phosphoribosyl-1-pyrophosphate on de novo pyrimidine biosynthesis in cultured Ehrlich ascites cells made permeable with dextran sulfate 500. J Biol Chem 254: 2697–2704

    PubMed  CAS  Google Scholar 

  • Chen J-J, Jones ME (1979b) Effect of 6-azauridine on de novo pyrimidine biosynthesis in cultured Ehrlich ascites cells. J Biol Chem 254; 4908–4914

    PubMed  CAS  Google Scholar 

  • Christopherson RI, Jones ME (1979) Interconversion of carbamyl-L-aspartate and L-dihydroorotate by dihydroorotase from mouse Ehrlich ascites carcinoma. J Biol Chem 254: 12506–12512

    PubMed  CAS  Google Scholar 

  • Christopherson RI, Matsuura T, Jones ME (1978) Radioassay of dihydroorotase utilizing ion-exchange chromatography. Anal Biochem 89: 225–234

    Article  PubMed  CAS  Google Scholar 

  • Coleman PF, Suttle DP, Stark GR (1977) Purification from hamster cells of the multifunctional protein that initiates de novo synthesis of pyrimidine nucleotides. J Biol Chem 252: 6379–6385

    PubMed  CAS  Google Scholar 

  • Coleman PF, Suttle DP, Stark GR (1978) Purification of a multifunctional protein bearing carbamyl-phosphate synthetase, aspartate tran carbamylase, and dihydroorotase enzyme activities from mutant hamster cells. In: Hoffee PA, Jones ME (eds) Methods in enzymology: Purine and pyrimidine nucleotide metabolism, vol LI, pp 121:134. Academic Press, New York

    Google Scholar 

  • Davis RH (1967) Channeling of Neurospora metabolism. In: Vogel HJ, Lampen LO, Bryson V (eds) Organizational biosynthesis, pp 303–322. Academic Press, New York

    Google Scholar 

  • Denis-Duphil M, Lacroute F (1971) Fine structure of the ura 2 locus in Saccharomyces cerevisiae. I In vivo complementation studies. Mol Gen Genet 112: 354–364

    Article  PubMed  CAS  Google Scholar 

  • Ennis HL, Lubin M (1963) Capacity for synthesis of a pyrimidine biosynthetic enzyme in mammalian cells. Biochim Biophys Acta 68: 78–83

    Article  CAS  Google Scholar 

  • Fallon HJ, Lotz M, Smith LH Jr (1962) Congenital orotic aciduria: demonstration of an enzyme defect in leukocytes and comparison with drug induced orotic aciduria. Blood 20: 700–709

    Google Scholar 

  • Fink GR (1971) Gene clusters and the regulation of biosynthetic pathways in Fungi. In: Vogel HJ (ed) Metabolic pathways, vol V, Metabolic regulation, pp 200–223. Academic Press, New York

    Google Scholar 

  • Fox RM, Royse-Smith D, O’Sullivan WJ (1970) Orotidinuria induced by allopurinol. Science 168: 861–862

    Article  PubMed  CAS  Google Scholar 

  • Galofre A, Kretchmer N (1970) Biosynthesis of pyrimidines by various organs of the chick during embryogenesis. Pediat Res 4: 55–62

    PubMed  CAS  Google Scholar 

  • Grobner W, Kelley WN (1975) Effect of allopurinol and its metabolic derivatives on the configuration of human orotate phosphoribosyltransferase and orotidine 5-phosphate decarboxylase. Biochem Pharmacol 24: 379–384

    Article  PubMed  CAS  Google Scholar 

  • Hager SE, Jones ME (1965) Initial step in pyrimidine synthesis in Ehrlich ascites carcinoma in vitro. I. Factors affecting incorporation of 14C-bicarbonate into carbon 2 of the uracil ring of the acid-soluble nucleotides of intact cells. J Biol Chem 240: 4556–4569

    PubMed  CAS  Google Scholar 

  • Hager SE, Jones ME (1967a) Initial steps in pyrimidine synthesis in Ehrlich ascites carcinoma in vitro. II. The synthesis of carbamyl phosphate by a soluble glutamine dependent carbamyl phosphate synthetase. J Biol Chem 242: 5667–5673

    PubMed  CAS  Google Scholar 

  • Hager SE, Jones ME (1967b) A glutamine-dependent enzyme for the synthesis of carbamyl phosphate for pyrimidine biosynthesis in fetal rat liver. J Biol Chem 242: 5674–5680

    PubMed  CAS  Google Scholar 

  • Hatfield D, Wyngaarden JB (1964) 3-Ribosylpurines. I. Synthesis of (3-ribosyluric acid) 5’-phosphate and (3-ribosylxanthine) 5’-phosphate by a pyrimidine ribonucleotide pyrophosphorylase of beef erythrocytes. J Biol Chem 239: 2580–2586

    PubMed  CAS  Google Scholar 

  • Hitchings GH (1973) Indications for control mechanisms in purine and pyrimidine biosynthesis as revealed by studies with inhibitors. In: Weber G (ed) Advances in enzyme regulation, vol XII, pp 121–129. Pergamon Press, Oxford New York

    Google Scholar 

  • Hoogenraad NJ, Levine RL, Kretchmer N (1971) Copurification of carbamoyl phosphate synthetase and aspartate transcarbamylase from mouse spleen. Biochem Biophys Res Commun 44: 981–988

    Article  PubMed  CAS  Google Scholar 

  • Ito K, Uchino H (1971) Control of pyrimidine biosynthesis in human lymphocytes. Induction of glutamine-utilizing carbamyl phosphate synthetase and operation of orotic acid pathway during blastogenesis. J Biol Chem 246: 4060–4065

    PubMed  CAS  Google Scholar 

  • Janeway CM, Cha S (1977) Effects of 6-azauridine on nucleotides, orotic acid, and orotidine in L5178Y mouse lymphoma cells in vitro. Cancer Res 37: 4382–4388

    PubMed  CAS  Google Scholar 

  • Jones ME (1970) Vertebrate carbamyl phosphate synthetase I and II. Separation of the arginineurea and pyrimidine pathways. In: Schmidt-Nelson B (ed) Urea and the kidney, pp 35–47. Excerpta Medica Foundation, International Congress Series No. 195, Amsterdam

    Google Scholar 

  • Jones ME (1971) Regulation of pyrimidine and arginine biosynthesis in mammals. In: Weber G (ed) Advances in enzyme regulation, vol IX, pp 19–49. Pergamon Press, Oxford New York

    Google Scholar 

  • Jones ME (1972) Regulation of uridylic acid biosynthesis in eukaryotic cells. In: Horecker B, Stadtman ER (eds) Current topics on cellular regulation, vol VI, pp 227–265. Academic Press, New York

    Google Scholar 

  • Jones ME, Kavipurapu PR, Traut TW (1978) Orotate phosphoribosyltransferase: orotidylate decarboxylase (Ehrlich ascites cell). In: Hoffee PA, Jones ME (eds) Methods in enzymology: Purine and pyrimidine nucleotide metabolism, vol LI, pp 155–167. Academic Press, New York

    Chapter  Google Scholar 

  • Jund R, Lacroute F (1972) Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae. J Bacteriol 109: 196–202

    PubMed  CAS  Google Scholar 

  • Kasebekar DK, Nagabhushanam A, Greenberg DM (1964) Purification and properties of orotic acid-decarboxylating enzyme from calf thymus. J Biol Chem 239: 4245–4249

    Google Scholar 

  • Kavipurapu PR, Jones ME (1976) Purification, size and properties of the complex of orotate phosphoribosyltransferase: orotidylate decarboxylase from mouse Ehrlich ascites carcinoma. J Biol Chem 251: 5589–5599

    PubMed  CAS  Google Scholar 

  • Kelley WN, Beardmore TJ (1970) Allopurinol: alteration in pyrimidine metabolism in man. Science 169: 388–390

    Article  PubMed  CAS  Google Scholar 

  • Kempe TD, Swyryd EA, Bruist M, Stark GR (1976) Stable mutants of mammalian cells that overproduce the first three enzymes of pyrimidine nucleotide biosynthesis. Cell 9: 541–550

    Article  PubMed  CAS  Google Scholar 

  • Kennedy J (1974) Dihydroorotase from rat liver: Purification properties and regulatory role in pyrimidine biosynthesis. Arch Biochem Biophys 160: 358–365

    PubMed  CAS  Google Scholar 

  • Kim S, Cohen PP (1965) Transcarbamylase activity in fetal liver and in liver of partially hepatectomiz-ed parabiotic rats. Arch Biochem Biophys 109: 421–428

    Article  PubMed  CAS  Google Scholar 

  • Kirschner K, Bisswanger H (1976) Multifunctional proteins. Annu Rev Biochem 45: 143–166

    Article  PubMed  CAS  Google Scholar 

  • Levenberg B (1962) Role of L-glutamine as donor of carbamyl nitrogen for the enzymatic synthesis of citrulline in Agaricus bisporus. J Biol Çhem 237: 2590–2598

    PubMed  CAS  Google Scholar 

  • Levinson BB, Ullman B, Martin DW Jr (1979) Pyrimidine pathway variants of cultured mouse lymphoma cells with altered levels of both orotate phosphoribosyltransferase and orotidylate decarboxylase. J Biol Chem 254: 4396–4401

    PubMed  CAS  Google Scholar 

  • Levitsky A, Koshland DE Jr (1971) Cytidine triphosphate synthetase. Covalent intermediates and mechanisms of action. Biochemistry 10: 3365–3371

    Article  Google Scholar 

  • Makoff AJ, Radford A (1978) Genetics and biochemistry of carbamoyl phosphate biosynthesis and its utilization in the pyrimidine biosynthetic pathway. Microbiol Rev 42: 307–328

    PubMed  CAS  Google Scholar 

  • Matsuura T, Jones ME (1974) Subcellular localization in the Ehrlich ascites cells of the enzyme which oxidizes dihydroorotate to orotate. In: Richter D (ed) Lipmann Symposium: Energy, biosynthesis and regulation in molecular biology, pp 422–434. de Gruyter, Berlin New York

    Google Scholar 

  • Miller RW (1975) A high molecular weight dehydroorotate dehydrogenase of Neurospora crassa. Purification and properties of the enzyme. Can J Biochem 53: 1288–1300

    Article  PubMed  CAS  Google Scholar 

  • Mori M, Tatibana M (1973) Dissociation by elastase digestion of the enzymes complex catalyzing the initial steps of pyrimidine biosynthesis in rat liver. Biochem Biophys Res Commun 54: 1525–1531

    Article  PubMed  CAS  Google Scholar 

  • Mori M, Tatibana M (1975) Purification of homogeneous glutamine-dependent carbamyl phosphate synthetase from ascites hepatoma cells as a complex with aspartate transcarbamylase and dihydroorotase. J Biochem 78: 239–242

    PubMed  CAS  Google Scholar 

  • Mori M, Tatibana M (1978a) Multi-enzyme complex of glutamine-dependent carbamoyl-phosphate synthetase with aspartate carbamoyltransferase and dihydroorotase from rat ascites-hepatoma cells. Eur J Biochem 86: 381–388

    Article  PubMed  CAS  Google Scholar 

  • Mori M, Tatibana M (1978b) A multienzyme complex of carbamoyl-phosphate synthetase (glutamine): aspartate carbamoyltransferase: dihydroorotase (rat ascites hepatoma cells and rat liver). In: Hoffee PA, Jones ME (eds) Methods in enzymology: Purine and pyrimidine nucleotide metabolism, vol LI, pp 111–121. Academic Press, New York

    Chapter  Google Scholar 

  • Natalini P, Ruggieri S, Santarelli I, Vita A, Magni G (1979) Baker’s yeast UMP: pyrophosphate phosphoribosyltransferase. Purification enzymatic and kinetic properties. J Biol Chem 254: 1558–1563

    PubMed  CAS  Google Scholar 

  • Nørby S (1970) A specific nutritional requirement for pyrimidine in rudimental mutants of Drosophila melanogaster. Hereditas 66: 205–214

    Article  PubMed  Google Scholar 

  • O’Donovan GA, Neuhard J (1970) Pyrimidine metabolism in microorganisms. Bacteriol Rev 34: 278–343

    PubMed  Google Scholar 

  • Pasternak CA, Handschumacher RE (1959) The biochemical activity of 6-azauridine: interference with pyrimidine metabolism in transplantable mouse tumors. J Biol Chem 234: 2992–2997

    PubMed  CAS  Google Scholar 

  • Pinsky L, Krooth RS (1967a) Studies on the control of pyrimidine biosynthesis in human diploid cell strains. I. Effect of 6-azauridine on cellular phenotype. Proc Natl Acad Sci USA 57: 925–932

    Article  PubMed  CAS  Google Scholar 

  • Pinsky L, Krooth RS (1967b) Studies on the control of pyrimidine biosynthesis in human diploid cell strains. II. Effects of 5-azaorotic acid, barbituric acid and pyrimidine precursors on cellular phenotype. Proc Natl Acad Sci USA 57: 1267–1274

    Article  PubMed  CAS  Google Scholar 

  • Rawls JM, Fristrom JW (1975) A complex genetic locus that controls the first three steps of pyrimidine biosynthesis in Drosophila. Nature 255: 738–740

    Article  PubMed  CAS  Google Scholar 

  • Reichard P (1959) The enzymatic synthesis of pyrimidines. In: Nord FF (ed) Advances in enzymology, vol XXI, pp 263–294. Interscience, New York

    Google Scholar 

  • Reichard P, Hanshoff G (1956) Aspartate carbamyl transferase from Escherichia coli. Acta Chem Scand 10: 548–566

    Article  CAS  Google Scholar 

  • Reyes P, Guganig ME (1975) Studies on a pyrimidine-phosphoribosyl-transferase for murine leukemia P1534J. Partial purification, substrate specificity, and evidence for its existence as a bifunctional complex with orotidine 5′-phosphate decarboxylase. J Biol Chem 250: 5097–5108

    PubMed  CAS  Google Scholar 

  • Reyes P, Intress C (1978) Coordinate behavior of orotate phosphoribosyl-transferase and orotidylate decarboxylase in mouse liver and brain. Life Sci 22: 577–582

    Article  PubMed  CAS  Google Scholar 

  • Shoaf WT, Jones ME (1971) Initial steps in pyrimidine synthesis in Ehrlich ascites carcinoma. Biochem Biophys Res Commun 45: 796–802

    Article  PubMed  CAS  Google Scholar 

  • Shoaf WT, Jones ME (1972) Uridylic acid synthesis in Ehrlich ascites carcinoma. Properties, subcellular distribution, and nature of enzyme complexes of the six biosynthetic enzymes. Biochemistry 12: 4039–4051

    Article  Google Scholar 

  • Smith LH Jr, Baker FA (1959) Pyrimidine metabolism in man. I. The biosynthesis of orotic acid. J Clin Invest 38: 798–809

    Article  PubMed  CAS  Google Scholar 

  • Smith LH Jr, Baker FA (1960) Pyrimidine metabolism in man. III. Studies on the leukocytes and erythrocytes in pernicious anemia. J Clin Invest 39: 15–20

    Article  PubMed  CAS  Google Scholar 

  • Smith LH Jr, Gilmour L (1975) Determination of urinary carbamyl-aspartate and dihydroorotate in normal subjects and in patients with hereditary orotic aciduria. J Lab Clin Med 86: 1047–1051

    PubMed  CAS  Google Scholar 

  • Smith LH Jr, Huguley CM Jr, Bain JA (1966) Hereditary orotic aciduria. In: Stanbury JB, Wyngaarden JB, Fredrickson DS (eds) The metabolic basis of inherited disease, 2nd ed, pp 739–758. McGraw-Hill, New York

    Google Scholar 

  • Suttle DP, Stark GR (1979) Coordinate overproduction of orotate phosphpribosyltransferase and orotidine-5’-phosphate decarboxylase in hamster cells resistant to pyrazofurin and 6-azauridine. J Biol Chem 254: 4602–4607

    PubMed  CAS  Google Scholar 

  • Sweeney MJ, Hoffman DH, Poore GA (1971) Enzymes in pyrimidine biosynthesis. Adv Enzyme Regul 9:51–61

    Article  Google Scholar 

  • Tallan HH, Moore S, Stein WH (1956) N-Acetyl-L-aspartic acid in brain. J Biol Chem 219: 257–264

    PubMed  CAS  Google Scholar 

  • Tatibana M, Ito K (1969) Control of pyrimidine biosynthesis in mammalian tissues. I. Partial purification and characterization of glutamine-utilizing carbamyl phosphate synthetase of mouse spleen and its tissue distribution. J Biol Chem 244: 5403–5413

    PubMed  CAS  Google Scholar 

  • Tatibana M, Shigesada K (1972) Activation by 5-phosphoribosyl 1-pyrophosphate of glutamine-dependent carbamyl phosphate synthetase from mouse spleen. Biochem Biophys Res Commun 46: 491–497

    Article  PubMed  CAS  Google Scholar 

  • Taylor AL, Trotter CD (1972) Linkage map of Escherichia coli strain K12. Bacteriol Rev 36: 504–524

    PubMed  CAS  Google Scholar 

  • Taylor WH, Taylor ML, Eames DF (1966) Two functionally different dihydroorotic dehydrogenases in bacteria. J Bacteriol 91: 2251–2256

    PubMed  CAS  Google Scholar 

  • Traut TW (1980) Significance of the enzyme complex that synthesizes UMP in Ehrlich ascites cells. Arch Biochem Biophys 200: 590–594

    Article  PubMed  CAS  Google Scholar 

  • Traut TW, Jones ME (1977a) Inhibitors of orotate phosphoribosyltransferase and orotidine-5′-phosphate decarboxylase from mouse Ehrlich ascites cells: a procedure for analyzing the inhibition of a multi-enzyme complex. Biochem Pharmacol 26: 2291–2296

    Article  PubMed  CAS  Google Scholar 

  • Traut TW, Jones ME (1977b) Kinetic and conformational studies of the orotate phosphoribosyltransferase: orotidine-5’-phosphate decarboxylase enzyme complex from mouse Ehrlich ascites cells. J Biol Chem 252: 8374–8381

    CAS  Google Scholar 

  • Traut TW, Jones ME (1978) Mammahan synthesis of UMP from orotate: the regulation of and con-formers of Complex U. In: Weber G (ed) Advances in enzyme regulation, vol XVI, pp 21–41. Pergamon Press, Oxford New York

    Google Scholar 

  • Traut TW, Jones ME (1979) Interconversion of different molecular weight forms of the orotate phosphoribosyltransferase orotidine-5’-phosphate decarboxylase enzyme complex from mouse Ehrlich ascites cells. J Biol Chem 254: 1143–1150

    PubMed  CAS  Google Scholar 

  • Weber G, Queener SF, Ferdinandus JA (1971) Control of gene expression in carbohydrate, pyrimidine and DNA metabolism. In: Weber G (ed) Advances in enzyme regulation, Vol IX, pp 65–95. Pergamon Press, Oxford New York

    Google Scholar 

  • Williams LG, Bernhardt PH, Davis RH (1970) Copurification of pyrimidine-specific carbamyl phosphate synthetase and aspartate transcarbamylase of Neurospora crassa. Biochemistry 9: 4329–4335

    Article  PubMed  CAS  Google Scholar 

  • Yip MCM, Knox WE (1970) Glutamine-dependent carbamyl phosphate synthetase. Properties and distribution in normal and neoplastic rat tissue. J Biol Chem 245: 2199–2204

    PubMed  CAS  Google Scholar 

  • Young JE, Prager MD, Atkins IC (1967) Comparative activities of aspartate transcarbamylase in various tissues of the rat. Proc Soc Exp Biol Med 125: 860–862

    PubMed  CAS  Google Scholar 

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  1. Department of Biochemistry and Nutrition, School of Medicine, University of North Carolina, Chapel Hill, NC, 27514, USA

    M. E. Jones

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  1. CNRS, Inst. de recherche en biologie moléculaire, Université Paris VII, Tour 43, 2 Place Jussieu, F-75221, Paris Cedex 05, France

    F. Chapeville  & A.-L. Haenni  & 

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Jones, M.E. (1980). The Genes for and Regulation of the Enzyme Activities of two Multifunctional Proteins Required for the De Novo Pathway for UMP Biosynthesis in Mammals. In: Chapeville, F., Haenni, AL. (eds) Chemical Recognition in Biology. Molecular Biology, Biochemistry and Biophysics, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81503-4_13

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