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Prediction of Clinical Response to 5-Fluorouracil-Containing Chemotherapy: Preliminary Results of in Vitro Assay in Human Breast Cancer

  • B. Ardalan
  • J. Macdonald
  • D. Cooney
  • M. Lippman
  • P. Schein
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 74)

Summary

An in vitro assay system measuring the production of phosphorylated nucleotide products of 5-FU by human tumor cytosols is described. Samples from 11 patients with breast cancer treated with 5-FU-containing chemotherapy regimens were assayed. Significant differences were seen in quantitative and qualitative patterns of phosphorylation between samples from chemotherapy responding and nonresponding patients. The rate of 5-fluorodeoxyuridylate production and the amount of nucleotide triphosphate produced were significantly P < 0.05 increased in chemotherapy-re- sponding patients. This in vitro assay system is a simple, reproducible procedure that requires further evaluation as a potential system for prospectively predicting clinical response to fluorinated pyrimidines.

Keywords

Human Breast Cancer Cytosol Fraction Tritiated Water Pyrimidine Nucleoside Combination Chemotherapy Regimen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • B. Ardalan
  • J. Macdonald
  • D. Cooney
  • M. Lippman
  • P. Schein

There are no affiliations available

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