Prediction of Clinical Response to 5-Fluorouracil-Containing Chemotherapy: Preliminary Results of in Vitro Assay in Human Breast Cancer
An in vitro assay system measuring the production of phosphorylated nucleotide products of 5-FU by human tumor cytosols is described. Samples from 11 patients with breast cancer treated with 5-FU-containing chemotherapy regimens were assayed. Significant differences were seen in quantitative and qualitative patterns of phosphorylation between samples from chemotherapy responding and nonresponding patients. The rate of 5-fluorodeoxyuridylate production and the amount of nucleotide triphosphate produced were significantly P < 0.05 increased in chemotherapy-re- sponding patients. This in vitro assay system is a simple, reproducible procedure that requires further evaluation as a potential system for prospectively predicting clinical response to fluorinated pyrimidines.
KeywordsHuman Breast Cancer Cytosol Fraction Tritiated Water Pyrimidine Nucleoside Combination Chemotherapy Regimen
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- 8.Livingston RB, Carter SK (1970) Single agents in cancer chemotherapy. Plenum, New YorkGoogle Scholar
- 10.Sawyer R, Nayak R, Spiegelman S, Martin D (1979) Mechanism of action of 5-fluorouracil (FU) in the chemotherapy of the murine mammary tumor. Proc Am Assoc Cancer Res and Am Soc Clin Oncol 20:263Google Scholar