Effect of Bicarbonate and Inhibitors on the 113Cadmium NMR Signals in Zinc-Replaced Human Carbonic Anhydrase
Nmr spectra of single 113Cd ions substituted for Zn in the active site of human carbonic anhydrase B in aqueous solution are obtained at 2.11 Tesla. Bicarbonate is involved in an exchange process which can lead to broadening and shifting of the mar lines. Bromide ion gives two peaks which are assigned to inner-sphere and outer-sphere binding.
KeywordsFTNMR Spectrometer Human Carbonic Anhydrase Anion Binding Site Single 113Cd Coalescence Point
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© Springer-Verlag Berlin Heidelberg 1979