Effect of Bicarbonate and Inhibitors on the 113Cadmium NMR Signals in Zinc-Replaced Human Carbonic Anhydrase

  • J. L. Sudmeier
  • J. L. Evelhoch
  • U. Carlsson
Conference paper

Abstract

Nmr spectra of single 113Cd ions substituted for Zn in the active site of human carbonic anhydrase B in aqueous solution are obtained at 2.11 Tesla. Bicarbonate is involved in an exchange process which can lead to broadening and shifting of the mar lines. Bromide ion gives two peaks which are assigned to inner-sphere and outer-sphere binding.

Keywords

Zinc Cadmium Bromide Iodide Bicarbonate 

References

  1. 1.
    J.L. Sudmeier and S.J. Bell, J.Amer.Chen.Soc., 99, 4499 (1977).Google Scholar
  2. 2.
    I.M. Armitage et. al., ibid., 98, 5710 (1976).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • J. L. Sudmeier
    • 1
    • 2
  • J. L. Evelhoch
    • 1
    • 2
  • U. Carlsson
    • 2
    • 1
  1. 1.Dept. of ChemistryUniv. of Calif.RiversideUSA
  2. 2.IFM/Dept. of ChemistryLinköping UniversityLinköpingSweden

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