Proton Pumping Across the Thylakoid Membrane Resolved in Time and Space

  • W. Junge
  • A. J. McGeer
  • W. Ausländer
  • J. Kollia
Part of the Colloquium der Gesellschaft für Biologische Chemie book series (MOSBACH, volume 29)

Abstract

For more than a decade the pathway of energy flow in photophosphorylation and in oxidative phosphorylation has been under debate. After the discussion on whether or not protons are obligatorily involved faded away [for an “afterglow” see the multiauthored review by Boyer et al. (1)] in favor of the causality sequence, electrons-protons-ATP, postulated by Mitchell (2,3), another controversy was revived. The question is whether the protons drive ATP synthesis via the osmolar volumes [Mitchell (2,3,4,5)in his chemiosmotic hypothesis] or whether they are confined to special conducting subspaces within the membrane [Williams (6,7,8,9) in his localized proton hypothesis]. In the public debate on the matter (4,5,8,9) the weight seems to be on the side of the chemiosmotic version. Especially in chloroplasts, there is no doubt that electron transport is coupled to inwardly directed proton pumping [for reviews, see Refs. [(10,11,12,13)] and that protons placed in the internal osmolar volume can be used for ATP synthesis [for a review, see Ref. (14)]. However, there are a few observations that seem to shed doubt on the chemiomotic concept or, at least, make it worthwhile to take a closer look at localized protons:

Keywords

Chlorophyll Photosynthesis HEPES Alkalinity Sorbitol 

Abbreviations

DBMIB

dibromothymoquinone; PS II photosystem two

ACES

N-(2-acetamido)-2-aminoethanesulfonic acid

HEPES

N-2hydroxyethylioerazine-N-2-ethanesulfonic acid

MES

2-(N-morpholino)-ethanesulfonic acid

MOPS

morpholinopropane sulfonic acid

PIPES

piperazine-N,N-Bis(2-ethanesulfonic acid)

PPi

pyrophosphate

TES

N-Tris(hydroxymethyl)methy1-2-amino-ethanesulfonic acid

Tricine

N-Tris(hydroxymethyl)methylglycine

Tris

Tris(hydroxymethyl)aminomethane

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Copyright information

© Springer-Verlag Berlin Heidelberg 1978

Authors and Affiliations

  • W. Junge
  • A. J. McGeer
  • W. Ausländer
  • J. Kollia

There are no affiliations available

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