Dynamic Morphology of Human Lymphoid Leukemias

Abstract

In descriptions of scanning electron micrographs of normal and leukemic white blood cells, little thought has been given to the fact that surface morphology and shape are a function of cell motility and, thus, subject to frequent changes. Consideration of these facts necessitates the combination of scanning electron microscopy (SEM) with microcinematography (MCM) under identical experimental conditions. Bessis and De Boisfleury [1, 2] were the first to utilize this approach for studies on white cell movements. We have applied the two methods for the analysis of cell populations from human acute and chronic leukemia and for two transplantable rat leukemias [3, 4].