Active Immunotherapy in Leukemia With Neuraminidase-Modified Leukemic Cells*
The enzymic cleavage of the terminal sugar residue from the plasma membrane of neoplastic cells by neuraminidase of vibrio cholerae origin causes a marked increase in immunogenicity of most experimental tumors [1–6, 8–16]. In a DBA/2 leukemia L1210 system, a single leukemic cell is capable of producing leukemia and the ultimate death of the host. However, mice which were immunized with neuraminidase-treated leukemia L1210 cells remained protected against a challenge of 100,000 untreated leukemic cells [1–3]. Repeated injections of neuraminidase-treated leukemia L1210 cells reduced the lethality rate and increased survival of mice with leukemia L1210 tumor grafts [15, 16]. Chemotherapy plus neuraminidase-treated L1210 cells administered in a sequence which preserves the host’s immunobiologically competent cells resulted in the cure of a significant portion of immunized mice [4, 7, 9]. The immunoprotection evoked by the stimulation from the neuraminidase-treated tumor cells was specific for the particular tumor and could be transferred by sera or splenic lymphocytes into unimmunized syngeneic mice [5, 7, 15, 16]. Recently, considerable attention has been directed to combined modality therapy in spontaneous Gross virus-induced leukemia in AkR mice because in many respects AkR leukemia has similar characteristics to the human leukemias.
KeywordsAcute Myelocytic Leukemia L1210 Cell Acute Myelocytic Leukemia Patient Remission Duration Active Immunotherapy
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