Abstract
From the X-ray diffraction studies of KIM et al. (1974) and ROBERTUS et al. (1974) a detailed picture of the crystal structure of phenylalanine-tRNA from yeast (tRNA Pheyeast ) has emerged. The finding that most of the hydrogen bonds between loop and stem regions which stabilize the tertiary structure of tRNAphe involve bases of the invariable parts of the primary sequences of tRNA suggests a similarity of the tertiary structure of all tRNAs. It is generally assumed that the crystal structure is predominant also in solution. The support for this assumption is mainly based on the accessibility of nucleotides to chemical modification, and has been reviewed by RICH and RAJBHANDARY (1976). Experiments in which the binding of oligonucleotides to tRNA was investigated, however, indicate interaction between oligonucleotides and complementdry sequences particularly in the D-loop (UHLENBECK, 1972; CAMERON and UHLENBECK, 197 3) and the anticodon-loop (UHLENBECK, 1972; EISINGER and SPAHR, 1973) which are not accessible in the crystal lattice. Studies of the solution structure of tRNA applying spectroscopic techniques such as low-angle X-ray diffraction (KRIGBAUM and GOODWIN, 1966; CONNORS et al., 1969; PILZ et al., 1970) and NMR (LIGHTFOOT et al., 1973; SHULMAN et al., 1973; DANIEL and COHN, 19 75; REID et al., 1975; REID and ROBILLARD, 1975; KEARNS, 1976) were up to now not able to decide whether conformations differing from the crystal structure are present in solution.
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Rigler, R., Ehrenberg, M., Wintermeyer, W. (1977). Structural Dynamics of tRNA A Fluorescence Relaxation Study of tRNA Pheyeast . In: Pecht, I., Rigler, R. (eds) Chemical Relaxation in Molecular Biology. Molecular Biology Biochemistry and Biophysics, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81117-3_6
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