Tissue Culture and Immunological Studies

  • Henry Basil Marsden
  • James Kenrick Steward
Part of the Recent Results in Cancer Research / Fortschritte der Krebsforschung / Progrès dans les recherches sur le cancer book series (RECENTCANCER, volume 13)


It was pointed out in Chapter 1 that an essential requirement of any service for children with tumours is to attract research workers to the problems of these patients. Clinical, epidemiological, and laboratory research are complementary and, largely due to the efforts of MILLER, FRAUMENI and DAVIES in America and STEWART in Britain, much epidemiological success has been achieved in paediatric oncology. However, closer collaboration between paediatric oncologists and basic laboratory cancer research is desirable to enable children to obtain full advantage from developments in the latter field. Based on the experience in Manchester the following ways of improving collaboration are suggested:
  1. 1.

    The wards where the children are treated must be within easy walking distance of the research laboratories so that there is a continual interchange of ideas and support.

  2. 2.

    Ideally, the paediatric oncologist would want to know of any technique which could be applied to children. However, basic cancer research is progressing at such a rate that it is difficult for anyone to keep up with more than a small part of it. A possible way of overcoming this difficulty would be for some sections (e. g. immunology, virology) in a research institute to have one member permanently assigned to paediatric oncology. There should also be career prospects for medically qualified paediatric oncologists who are academically minded and have received the training which makes them capable of spending much of their time in laboratory research.



Acute Leukaemia Paediatric Oncology Plasma Clot Glial Tumour Tumour Immunology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. ASHWOOD-SMITH, M. J., VOSS, W. A. G., WARBY, C.: Cryobiol. 10, 502 (1973)CrossRefGoogle Scholar
  2. ASTALDI, E., MASSIMO, L., AIRO, R., MARI, P. G.: Lancet 1, 1265 (1966)PubMedCrossRefGoogle Scholar
  3. BACH, M. L., BACH, F. H., JOO, P.: Science 166, 1520 (1969)PubMedCrossRefGoogle Scholar
  4. BAKKERA, J. A. J. M, DEVAAN, G. A. M., SCHRETTER, E. D. A. M., SGAND, J.: Haemat. 9, 36 (1972)Google Scholar
  5. BALDWIN, R. W., PRICE, M. R., ROBINS, R. A.: Int. J. Cancer 11, 527 (1973)PubMedCrossRefGoogle Scholar
  6. BARTH, R. F., GILLESPIE, G. Y., III, GOBUTY, A.: Nat. Cancer Inst. Monogr. 35, 39 (1972)PubMedGoogle Scholar
  7. BASSERGA, R., MALAMUD, D.: Autoradiography, Techniques and Application, New York: Harper and Row, 1969Google Scholar
  8. BEAN, M. A., PEES, H., ROSEN, G., OETTGEN, H. F.: Nat. Cancer Inst. Monogr. 37, 41 (1973)PubMedGoogle Scholar
  9. BLUMING, A. L., VOGEL, C. L., ZIEGLER, J. L., MODY, N., KAMYA, G.: Ann. Intern. Med. 76, 405 (1972)PubMedGoogle Scholar
  10. BRUNNER, K. T., MAUEL, J., CEROTTINI, J. C., CHAPUIS, B.: Immunology 14, 181 (1968)PubMedGoogle Scholar
  11. BURNET, F. M.: Immunological Surveillance. London: Pergamon Press, 1970Google Scholar
  12. BURTIN, P., GENDRON, M. C.: Proc. Nat. Acad. Sci. USA 70, 2051 (1973)PubMedCrossRefGoogle Scholar
  13. CAMPBELL, A. C., HERSEY, P., MACLENNAN, I. C. M., KAY, H. E. H., PIKE, M. C.: BMJ 2, 383 (1973)Google Scholar
  14. CHAR, D. H., LEPOURHIET, A., LEVENTHAL, B. G., HERBERMAN, R. B.: Int. J. Cancer 12 (2), 409 (1973)PubMedCrossRefGoogle Scholar
  15. COHEN, A. M., MILLAR, R. C., KETCHAM, A. S.: Cancer REs. 32, 2421 (1972)PubMedGoogle Scholar
  16. COONS, A. H., CREECH, H. J., JONES, R. N.: Proc. Soc. Exp. Biol. N. Y. 47, 200 (1941)Google Scholar
  17. COSTERO, I., POMERAT, C. M.: Cellular Protypes of central gliomata, Proc. 2nd Int. Cong. Neuropaths. London: Excerpta Medica, 1955, p. 273Google Scholar
  18. CROWTHER, D., POWLES, R. L., BATEMAN, C. J. T., BEARD, M. E. J., GAUCI, C. L., WRIGLEY, P. F. M., MALPAS, J. S., HAMILTON-FAIRLEY, G., BODLEY SCOTT, R. B.: BMJ 1, 131 (1973)PubMedCrossRefGoogle Scholar
  19. CURRIE, G. A.: Cancer and Immune Response, London: Edward Arnold, 1974Google Scholar
  20. DUPUY, J. M., KOURILSKY, F. M., FRADELIZZI, D., FEINGOLD, N., JACQUILLAT, C. E., BERNARD, J., DAUSSET, J.: Cancer 27, 323 (1971)PubMedCrossRefGoogle Scholar
  21. EHRMANN, R. L., GEY, G. O.: J. Nat. Cancer Inst. 16, 1375 (1956)PubMedGoogle Scholar
  22. EILBER, F. R., MORTON, D. L.: Cancer 25, 362 (1970)PubMedCrossRefGoogle Scholar
  23. EVERSON, T. C., COLE, W. H.: Spontaneous Regression of Cancer. Philadelphia: W. B. Saunders Co., 1966, Chap. 3Google Scholar
  24. FARRANT, J., SMITH, A.: Progress in Low Temperature Biology. Penguin Science Survey, B. A. Allison (ed.). Harmondsworth: Penguin Books, 1966Google Scholar
  25. FOLEY, E. J.: Cancer Res. 13, 835 (1953)PubMedGoogle Scholar
  26. FOLKMAN, J. L.: Advances in Cancer Research 19, 331 (1974)PubMedCrossRefGoogle Scholar
  27. FOLKMAN, J.: Ann. Int. Med. 82, 96 (1975)PubMedGoogle Scholar
  28. FOLKMAN, J., MERLER, E., ABERNATHY, C., WILLIAMS, G.: J. Exp. Med. 133, 275 (1971)PubMedCrossRefGoogle Scholar
  29. FREEMAN, C. B., HARRIS, R., GEARY, C. G., LEYLAND, M. J., MACIVER, J. E., DELAMORE, I.: BMJ 4, 571 (1973)PubMedCrossRefGoogle Scholar
  30. FRIDMAN, W. H., KOURILSKY, F. M.: Nature (LOND.) 224, 277 (1969)CrossRefGoogle Scholar
  31. GOLD, P., FREEDMAN, S. O.: J. Exp. MEd. 122, 467 (1965)PubMedCrossRefGoogle Scholar
  32. GROSS, L.: Cancer Res. 3, 326 (1943)Google Scholar
  33. GUTTERMAN, J. U., MAVLIGIT, G., GOTTLIEB, J. A., BURGESS, M. A., MCBRIDE, C. E., EINHORN, L., FREIREICH, E. J., HERSH, E. M.: New Eng. J. Med. 291, 592 (1974)PubMedCrossRefGoogle Scholar
  34. GUTTERMAN, J. U., ROSEN, R. D., BUTLER, W. J., MCCREDIE, K. B., BODEY, G. P., FREIREICH, E. J., HERSH, E. M.: New Eng. J. Med. 288, 169 (1973)PubMedCrossRefGoogle Scholar
  35. HAKALA, T. R., CASTRO, A. E., ELLIOT, A. Y., FRALEY, E. E.: Invest. Urol. 11, 405 (1974)PubMedGoogle Scholar
  36. HELLSTRÖM, K. E., HELLSTRÖM, I.: Advances in Immunology 18, 209 (1974)PubMedCrossRefGoogle Scholar
  37. HELLSTRÖM, I., HELLSTRÖM, K. E., PIERCE, G., BILL, A. H.: Proc. Nat. Acad. Sci. U. S. 60, 1231 (1968)CrossRefGoogle Scholar
  38. HELLSTRÖM, I., SJOGREN, H. O., WARNER, G. A., HELLSTRÖM, K. E.: Int. J. Cancer 7, 226 (1971)PubMedCrossRefGoogle Scholar
  39. HERBERMAN, H. R.: Fed. Proc, Fed. Amer. Soc. Exp. Biol. 32, 160 (1973)Google Scholar
  40. HERBERMAN, R. B.: Advances in Cancer Res. 19, 207 (1974)CrossRefGoogle Scholar
  41. HERSH, E. M., OPPENHEIM, J. J.: Cancer Res. 27, 98 (1967)PubMedGoogle Scholar
  42. HERSH, E. M., WHITECAR, J. P., MCCREDIE, W. B., BODEY, G. P., FREIREICH, E. J.: New Eng. J. Med. 285, 1211 (1971)PubMedCrossRefGoogle Scholar
  43. HEYN, R., BORGES, W., JOO, P., KARAN, M., NESBITT, M., SHORE, N.: Proc. Amer. Assoc. for Cancer Res. 14, 45 (1973)Google Scholar
  44. HUMPHREY, E. B., NESBITT, J. M. E., CHARY, K. K. N., KRIVIT, W.: Cancer 29, 402 (1972)PubMedCrossRefGoogle Scholar
  45. JAGARLAMOODY, S. M., AUST, J. C., TEW, R. H., MCKHANN, C. F.: Proc. Nat. Acad. Sci. U. S. 68, 1346 (1971)CrossRefGoogle Scholar
  46. JOSE, D. G., SKVARIL, F.: Int. J. Cancer 13, 173 (1974)PubMedCrossRefGoogle Scholar
  47. KERSTING, G.: Die Gewebszüchtung Menschlicher Hirngeschwulste, Berlin: Springer Verlag, 1961Google Scholar
  48. KLEIN, G.: Israel J. Med. Sci. 7, 111 (1971)PubMedGoogle Scholar
  49. KREDEL, F. E.: Arch. Surg. 18, 2008 (1929)CrossRefGoogle Scholar
  50. KUMAR, S., TAYLOR, G.: J. Clin. Path. 26, 476 (1973)PubMedCrossRefGoogle Scholar
  51. KUMAR, S., TAYLOR, G.: Int. J. Cancer 16, 448 (1975)PubMedCrossRefGoogle Scholar
  52. KUMAR, S., TAYLOR, G., STEWARD, J. K., WAGHE, M. A., JONES, P. H. M.: Int. J. Cancer 12, 194 (1973)PubMedCrossRefGoogle Scholar
  53. KUMAR, S., TAYLOR, G., STEWARD, J. K., WAGHE, M. A., PEARSON, D.: Int. J. Cancer 10, 36 (1972)PubMedCrossRefGoogle Scholar
  54. LARSAN, D. L., TOMLINSON, L. J.: J. Clin. Invest. 32, 317 (1953)CrossRefGoogle Scholar
  55. LEIGHTON, N. J.: Invasive Growth and Metastasis in Tissue Culture Systems. Methods in Cancer Research IV. H. Busch (ed.), New York: Academic Press, 1968, p. 85Google Scholar
  56. LEVENTHAL, B. G., HALTERMAN, R. H., ROSENBERG, E. B., HERBERMAN, R. B.: Cancer Res. 32, 820 (1972)Google Scholar
  57. LUMSDEN, C. E.: Tissue Culture in Relation to Tumours of the Nervous System. Pathology of Tumours of the Nervous System. D. S. Russell and L. J. Rubinstein (eds.). London: E. Arnold, 1971, p. 281Google Scholar
  58. MATHÉ, G.: Rev. Franc. Etud. Clin. Biol. 13, 881 (1968)PubMedGoogle Scholar
  59. MATHÉ, G.: Strategy for the treatment of acute leukaemia. In: Immunology. XIII. Int. Cong. Ped., Wien, 1971. Weiner Med. Akad., p. 21Google Scholar
  60. MATHÉ, G., AMIEL, J. L., SCHWARZENBERG, L., SCHNEIDER, M., CATTAN, A., SCHLUMBERGER, J. R., HAYAT, M., DE VASSAL, F.: Lancet 1, 697 (1969)PubMedCrossRefGoogle Scholar
  61. MAVLIGIT, G., AMBUS, U., GUTTERMAN, J. U., HERSH, E. M.: Nature (Lond.) New Biol. 243, 188 (1973)Google Scholar
  62. MAZUR, P., LEIBO, S. P., FARRANT, J., CHU, E. H. Y., HANNA, M. G. JR., SMITH, L. H.: The Frozen Cell. A Ciba Foundation Symposium. Wolstenholme and O’Connor (eds.). London: Churchill, 1970, p. 69Google Scholar
  63. MERYMAN, H. T.: Cryobiology. London: Academic Press, 1966Google Scholar
  64. MURRAY, M. R., STOUT, A. P.: Amer. J. Path. 19, 429 (1947)Google Scholar
  65. NAGAI, M., HOSHINA, T., TSUCHIDA, T., SATO, F., SANO, K.: Brain nerve (Tokyo) 19, 1055 (1967)Google Scholar
  66. NAGEL, G. A., PIESSENS, W. F., STILLMANT, M. M., LEJEUNE, F.: Europ. J. Cancer 7, 41 (1971)CrossRefGoogle Scholar
  67. NAIRN, R. C.: Fluorescent Protein Tracing. Edinburgh: E. & S. Livingstone Ltd., 1969Google Scholar
  68. OGRA, P. L., SINKS, L. F., KARZAN, D. T.: J. Ped. 79, 444 (1971)CrossRefGoogle Scholar
  69. OLD, L. J., CLARK, D. A., BENACERRAF, B.: Nature 184, 291 (1959)PubMedCrossRefGoogle Scholar
  70. OREN, M. E., HERBERMAN, R. B.: Clin. Exp. Immunol. 9, 45 (1971)PubMedGoogle Scholar
  71. PHILLIPS, P., STEWARD, J. K., KUMAR, S.: Int. J. Cancer (1976 in press)Google Scholar
  72. POMERAT, C. M.: J. Neuropath. 14, 28 (1955) Preliminary report of Medical Research Council on leukaemia in childhood (Concord trial). BMJ 4, 189 (1971)PubMedCrossRefGoogle Scholar
  73. ROSE, N. R., BONSTEIN, H. S.: Clin. Exp. Immunol. 7, 355 (1970)PubMedGoogle Scholar
  74. RUSSELL, D. S., BLAND, J. D. W.: J. Path. Bact. 36, 273 (1933)CrossRefGoogle Scholar
  75. SOKAL, J. E.: Cancer Res. 26, 1161 (1966)Google Scholar
  76. SOLOWEY, A. C., RAPAPORT, F. T.: Surg. Gynecol. Obstet. 121, 756 (1965)PubMedGoogle Scholar
  77. SOUTHAM, C. M.: Progr. Exp. Tumour Res. 9, 1 (1967)Google Scholar
  78. STEWARD, J. K.: In: Malignant Diseases in Children. T. Deeley (ed.). London: Butterworth’s, 1974, Chap. 3Google Scholar
  79. STEWARD, J. K., SMITH, J. L. S., ARNOLD, E. L.: B. J. Ophthalm 40, 449 (1956)CrossRefGoogle Scholar
  80. STOWELL, R. E.: Fed. Proc. 24, 5324 (1965Google Scholar
  81. TAYLOR, J. F., JUNGE, U., WOLFE, L.: Int. J. Cancer 8, 468 (1971)PubMedCrossRefGoogle Scholar
  82. VIZA, D. C., BERNARD-DEGANI, O., BERNARD, C., HARRIS, R. C.: Lancet 2, 493 (1969)CrossRefGoogle Scholar
  83. WALKER, J. S., DAVIS, D., FREEMAN, C. B., HAM, R.: Br. J. Cancer 27, 703 (1973)CrossRefGoogle Scholar
  84. YOUNG, R. C., CORDER, M. P., HAYNES, H. A., DE VITA, U. T.: Amer. J. Med. 52, 63 (1972)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1976

Authors and Affiliations

  • Henry Basil Marsden
    • 1
  • James Kenrick Steward
    • 2
    • 3
  1. 1.Department of Child Health, Christie HospitalUniversity of ManchesterWithington, ManchesterGreat Britain
  2. 2.Departments of Child Health and PathologyManchester UniversityUK
  3. 3.the Christie HospitalUK

Personalised recommendations