Abstract
In 1957 DALZIEL (1) described certain relationships that must be obeyed when initial rate experiments are carried out in both directions at a single pH for kinetically reversible bireactant systems. More recently, DALZIEL has extended this treatment to terreactant enzymes (2). The use of these relationships, called Φ relations, represents an important and powerful tool in enzyme kinetics for making a choice between certain kinetic mechanisms; however, its application requires very exact kinetic data and experimental reversibility. Another problem, which will be alluded to later, is that at least one type of kinetic mechanism, the rapid equilibrium Random Bi Bi mechanism, can in theory provide Φ relationships consistent with all those bireactant mechanisms that exhibit specific and unique Φ relationships.
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References
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© 1975 Springer-Verlag Berlin Heidelberg
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Fromm, H.J. (1975). Isomerization Mechanisms and the Φ and Haldane Relationships. In: Initial Rate Enzyme Kinetics. Molecular Biology Biochemistry and Biophysics, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80966-8_7
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DOI: https://doi.org/10.1007/978-3-642-80966-8_7
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