Advertisement

Product, Substrate, and Alternative Substrate Inhibition

  • Herbert J. Fromm
Part of the Molecular Biology Biochemistry and Biophysics book series (MOLECULAR, volume 22)

Abstract

The study of initial rate kinetics in the presence of product has proven to be a boon to the field of enzyme kinetics. Quite probably, no other single experimental protocol has received greater attention since its inception than product inhibition kinetics. Unfortunately, certain complicating factors, such as abortive ternary complex formation and substrate inhibition, have tended to limit the usefulness of this technique. Nevertheless, kinetic investigations in the presence of product remain a formidable tool available to the kineticist interested in making a choice of mechanism from among the usual possibilities. Furthermore, a good deal of insight may be obtained from such experiments regarding the binding of substrates and products at the active site and, in certain cases, at allosteric sites.

Keywords

Alternative Substrate Double Reciprocal Plot Ping Pong Mechanism Initial Reaction Velocity Yeast Hexokinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    ALBERTY, R.A.: J. Am. Chem. Soc. 80, 1777 (1958).CrossRefGoogle Scholar
  2. 2.
    ALBERTY, R.A.: J. Am. Chem. Soc. 75, 1928 (1953).CrossRefGoogle Scholar
  3. 3.
    CLELAND, W.W.: Biochim. Biophys. Acta 67, 104 (1963).PubMedCrossRefGoogle Scholar
  4. 4.
    DARVEY, I.G.: Biochem. J. 128, 383 (1972).PubMedGoogle Scholar
  5. 5.
    FROMM, H.J., NELSON, D.R.: Federation Proc. 20, abs. 229 (1961).Google Scholar
  6. 6.
    FROMM, H.J., NELSON, D.R.: J. Biol. Chem. 237, 215 (1962).PubMedGoogle Scholar
  7. 7.
    FROMM, H.J.: Biochim. Biophys. Acta 52, 199 (1961).PubMedCrossRefGoogle Scholar
  8. 8.
    FROMM, H.J., ZEWE, V.: J. Biol. Chem. 237, 1661 (1962).PubMedGoogle Scholar
  9. 9.
    HOLBROOK, J.J., GÜTFREUND, H.: Febs Lett. 31, 157 (1973).PubMedCrossRefGoogle Scholar
  10. 10.
    WETTERMARK, G., BORGLUND, E., BROLIN, S.E.: Anal. Biochem. 22, 211 (1968).PubMedCrossRefGoogle Scholar
  11. 11.
    FROMM, H.J.: Eur. J. Biochem. 7, 385 (1969).PubMedCrossRefGoogle Scholar
  12. 12.
    SCHWERT, G.W.: Federation Proc. 13, abs. 971 (1954).Google Scholar
  13. 13.
    DALZIEL, K.: Trans. Faraday Soc. 54, 1247 (1958).CrossRefGoogle Scholar
  14. 14.
    CLELAND, W.W., WRATTEN, C.C.: The Mechanism of Action of Dehydrogenases, p. 103. Lexington: The University Press of Kentucky 1969.Google Scholar
  15. 15.
    RUDOLPH, F.B., FROMM, H. J.: J. Theor. Biol. 39, 363 (1973).PubMedCrossRefGoogle Scholar
  16. 16.
    RUDOLPH, F.B., FROMM, H. J.: J. Biol. Chem. 246, 6611 (1971).PubMedGoogle Scholar
  17. 17.
    DELAFUENTE, G., SOLS, A.: Eur. J. Biochem. 16, 234 (1970).PubMedCrossRefGoogle Scholar
  18. 18.
    ROBBINS, E.A., BOYER, P.D.: J. Biol. Chem. 224, 121 (1957).PubMedGoogle Scholar
  19. 19.
    FROMM, H.J., ZEWE, V.: J. Biolo Chem. 237, 3027 (1962).Google Scholar
  20. 20.
    NOAT, G., RICARD, J., BOREL, M., GOT, C.: Eur. J. Biochem. 5, 55 (1968).PubMedCrossRefGoogle Scholar
  21. 21.
    CLELAND, W.W.: The Enzymes 2, 1 (1970).CrossRefGoogle Scholar
  22. 22.
    HALDANE, J.B.S.: Enzymes, London: Longmans 1930.Google Scholar
  23. 23.
    DALZIEL, K.: Acta Chem. Scand. 11, 1706 (1957).CrossRefGoogle Scholar
  24. 24.
    DIXON, M., WEBB, E.C.: Enzymes. New York: Academic Press 1964.Google Scholar
  25. 25.
    WRATTEN, C.C., CLELAND, W.W.: Biochemistry 2, 935 (1963).PubMedCrossRefGoogle Scholar
  26. 26.
    RUDOLPH, F.B., FROMM, H.J.: Biochemistry 9, 4660 (1970).PubMedCrossRefGoogle Scholar
  27. 27.
    RICARD, J., NOAT, G., GOT, C., BOREL, M.: Eur. J. Biochem. 31, 14 (1972).PubMedCrossRefGoogle Scholar
  28. 28.
    RUDOLPH, F.B., FROMM, H.J.: Arch. Biochem. Biophys. 147, 515 (1971).PubMedCrossRefGoogle Scholar
  29. 29.
    FROMM, H.J.: Biochim. Biophys. Acta 81, 413 (1964).PubMedGoogle Scholar
  30. 30.
    ZEWE, V., FROMM, H.J., FABIANO, R.: J. Biol. Chem. 239, 1625 (1964).PubMedGoogle Scholar
  31. 31.
    WRATTEN, C.C., CLELAND, W.W.: Biochemistry 4, 2442 (1965).CrossRefGoogle Scholar
  32. 32.
    NORTHROP, D.B.: J. Biol. Chem. 244, 5808 (1969).PubMedGoogle Scholar
  33. 33.
    NORTHROP, D.B., WOOD, H.G.: J. Biol. Chem. 244, 5820 (1969).PubMedGoogle Scholar
  34. 34.
    CLELAND, W.W.: J. Biol. Chem. 248, 8353 (1973).PubMedGoogle Scholar
  35. 35.
    CHAO, J., JOHNSON, C.F., GRAVES, D.J.: Biochemistry 8, 1459 (1968).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1975

Authors and Affiliations

  • Herbert J. Fromm
    • 1
  1. 1.Biochemistry and Biophysics DepartmentIowa State UniversityAmesUSA

Personalised recommendations