Skip to main content
SpringerLink
Log in

Experimental Protocol and Plotting of Kinetic Data

  • Chapter
Initial Rate Enzyme Kinetics

Part of the book series: Molecular Biology Biochemistry and Biophysics ((MOLECULAR,volume 22))

Abstract

In order to carry out initial rate studies, it is necessary to be aware of those factors that affect the kinetics of the system under investigation. These include substrate and product concentration, pH, temperature, ionic strength, enzyme stability, activators and inhibitors.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. BERGMEYER, H.U.: Methods of Enzymatic Analysis. Weinheim: Verlag Chemie 1963.

    Google Scholar 

  2. TU, J.-I. JACOBSON, G.R., GRAVES, D.J.: Biochemistry 10, 1229 (1971).

    Article  PubMed  CAS  Google Scholar 

  3. RUDOLPH, F.B., PURICH, D.L., FROMM, H.J.: J. Biol. Chem. 243, 5539 (1968).

    PubMed  CAS  Google Scholar 

  4. DAVIS, B.J.: Ann. N.Y. Acad. Sci. 121B, 404 (1964).

    Google Scholar 

  5. SMITHIES, O.B.: Biochem. J. 61, 629 (1955).

    PubMed  CAS  Google Scholar 

  6. GROSSBARD, L., SCHIMKE, R.T.: J. Biol. Chem. 241, 3546 (1966).

    PubMed  CAS  Google Scholar 

  7. SILVERSTEIN, E., BOYER, P.D.: Anal. Biochem. 8, 470 (1964).

    Article  PubMed  CAS  Google Scholar 

  8. WOOLF, B.: quoted in: Allgemeine Chemie der Enzyme by J.B.S. Haldane and K.G. Stern. Dresden-Leipzig: Steinkopff 1932, S. 119.

    Google Scholar 

  9. LINEWEAVER, H., BURK, D.: J. Am. Chem. Soc. 56, 658 (1934).

    Article  CAS  Google Scholar 

  10. WILKINSON, G.N.: Biochem. J. 80, 324 (1961).

    PubMed  CAS  Google Scholar 

  11. FROMM, H.J.: Biochim. Biophys. Acta 139, 221 (1967).

    PubMed  CAS  Google Scholar 

  12. COHN, M.: Biochemistry 2, 623 (1963).

    Article  PubMed  CAS  Google Scholar 

  13. FROMM, H.J., SILVERSTEIN, E., BOYER, P.D.: J. Biol. Chem. 239, 3645 (1964).

    PubMed  CAS  Google Scholar 

  14. PURICH, D.L., FROMM, H.J.: J. Biol. Chem. 246, 3456 (1971).

    PubMed  CAS  Google Scholar 

  15. BACHELARD, H.S.: Biochem. J. 125, 249 (1971).

    PubMed  CAS  Google Scholar 

  16. HILL, A.V.: Biochem. J. 7, 471 (1913).

    PubMed  CAS  Google Scholar 

  17. PURICH, D.L., FROMM, H.J.: Biochem. J. 130, 63 (1972).

    PubMed  CAS  Google Scholar 

  18. GOOD, N.E., WINGET, G.D., WINTER, W., CONNOLLY, T.N., IZAWA, S., SING, R.M.M.: Biochemistry 5, 467 (1966).

    Article  PubMed  CAS  Google Scholar 

  19. BURTON, K.: Biochem. J. 71, 388 (1959).

    PubMed  CAS  Google Scholar 

  20. RUDOLPH, F.B., FROMM, H.J.: J. Biol. Chem. 244, 3832 (1969).

    PubMed  CAS  Google Scholar 

  21. O’SULLIVAN, W.J., PERRIN, D.D.: Biochemistry 3, 18 (1964).

    Article  PubMed  Google Scholar 

  22. NOAT, G., RICARD, J., BOREL, M., GOT, C.: Eur. J. Biochem. 5, 55 (1968).

    Article  PubMed  CAS  Google Scholar 

  23. SMITH, R.M., ALBERTY, R.A.: J. Am. Chem. Soc. 78, 2376 (1956).

    Article  CAS  Google Scholar 

  24. BOYER, P.D., SEGAL, H.L.: In: A Symposium on the Mechanism of Enzyme Action, p. 523. Baltimore, Md.: Johns Hopkins Press 1954.

    Google Scholar 

  25. MCCLURE, W.R.: Biochemistry 8, 2782 (1969).

    Article  PubMed  CAS  Google Scholar 

  26. DALZIEL, K.: Biochem. J. 80, 440 (1961).

    PubMed  CAS  Google Scholar 

  27. HANES, C.S.: Biochem. J. 26, 1406 (1932).

    PubMed  CAS  Google Scholar 

  28. HOFSTEE, B.H., DIXON, M., WEBB, E.C.: Nature 184, 1296 (1959).

    Article  PubMed  CAS  Google Scholar 

  29. DOWD, J.E., RIGGS, D.S.: J. Biol. Chem. 240, 863 (1965).

    PubMed  CAS  Google Scholar 

  30. BEVINGTON, P.R.: Data Reduction and Error Analysis for the Physical Sciences. New York: McGraw Hill 1969.

    Google Scholar 

  31. CLELAND, W.W.: Adv. Enzymol. 29, 1 (1967).

    PubMed  CAS  Google Scholar 

  32. HOHNADEL, D.C., COOPER, C.: Eur. J. Biochem. 31, 180 (1972).

    Article  PubMed  CAS  Google Scholar 

  33. HOCKING, R.R., LESLIE, R.N.: Technometrics 9, 531 (1967).

    Article  Google Scholar 

  34. EISENTHAL, R., CORNISH-BOWDEN, A.: Biochem. J. 139, 715 (1974).

    PubMed  CAS  Google Scholar 

  35. CORNISH-BOWDEN, A., EISENTHAL, R.: Biochem. J. 139, 721 (1974).

    PubMed  CAS  Google Scholar 

  36. DALZIEL, K.: Acta Chem. Scand. 11, 1706 (1957).

    Article  CAS  Google Scholar 

  37. FRIEDEN, C.: J. Am. Chem. Soc. 79, 1894 (1957).

    Article  CAS  Google Scholar 

  38. FLORINI, J.R., VESTLING, C.S.: Biochim. Biophys. Acta 25, 575 (1957).

    Article  PubMed  CAS  Google Scholar 

  39. FRIEDEN, C.: J. Biol. Chem. 234, 2891 (1959).

    PubMed  CAS  Google Scholar 

  40. GARCES, E., CLELAND, W.W.: Biochemistry 8, 633 (1969).

    Article  PubMed  CAS  Google Scholar 

  41. LUECK, J.D., ELLISON, W.R., FROMM, H.J.: Febs Letters 30, 321 (1973).

    Article  PubMed  CAS  Google Scholar 

  42. DELAFUENTE, G., SOLS, A.: Eur. J. Biochem. 16, 234 (1970).

    Article  PubMed  CAS  Google Scholar 

  43. NAGY, M., DJEMBO-TATY, M., HESLOT, H.: Biochim. Biophys. Acta 309, 1 (1973).

    PubMed  CAS  Google Scholar 

  44. SEGAL, H.L., KACHMAR, J.F., BOYER, P.D.: Enzymologia 15, 187 (1952).

    PubMed  CAS  Google Scholar 

  45. SHIMERLIK, M.I., CLELAND, W.W.: J. Biol. Chem. 248, 8418 (1973).

    Google Scholar 

  46. DALZIEL, K.: Biochem. J. 114, 547 (1969).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemistry and Biophysics Department, Iowa State University, Ames, IA, 50010, USA

    Professor Herbert J. Fromm Ph. D.

Authors
  1. Professor Herbert J. Fromm Ph. D.
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1975 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Fromm, H.J. (1975). Experimental Protocol and Plotting of Kinetic Data. In: Initial Rate Enzyme Kinetics. Molecular Biology Biochemistry and Biophysics, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80966-8_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-80966-8_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-80968-2

  • Online ISBN: 978-3-642-80966-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation