Parathyroid Hormone: Structure and Immunoheterogeneity
The amino acid sequence of the NH2-terminal 34 residues of human parathyroid hormone (HPTH) has been determined and duplicated synthetically to produce a peptide that is biologically active. In the amino acid sequences of the bovine and porcine hormones, the glutamic acid function at position 22 has been revised to glutamine. Among these initial 34 residues, HPTH differs from bovine PTH by 5 residues and from porcine PTH by 4 residues.
A synthetic preparation of HPTH 1-34 was used to characterize two antisera to PTH. One antiserum (CH 14M) reacted almost equally well with the synthetic peptide (HPTH 1-34) as with HPTH 1-84 obtained from natural sources. The other antiserum (GP 1M) did not react with the synthetic peptide (HPTH 1-34) but reacted with HPTH 1-84 to an identical degree as antiserum CH 14M. On this basis, antiserum CH 14M was designated “anti N” to describe its NH2-terminal specificity, and GP 1M was designated “anti C” to describe its COOH-terminal specificity.
Radioimmunoassays using these antisera were systematically applied to the measurement of serum immunoreactive PTH (iPTH) in patients with primary or secondary (renal) hyperparathyroidism. The results showed that the “anti C” assay system was superior to the “anti N” assay system in assessing both chronic parathyroid hyperfunction and the biologic effects of excess circulating PTH (osteoclast number in bone biopsies). In contrast, reference is made to the superiority of the “anti N” assay system in assessing acute changes in parathyroid function and in localizing abnormal parathyroid tissue by the method of differential catheterization of the venous drainage of the neck and mediastinum.
An explanation for these important differences in the results obtained with the two assay systems is based on observations that the concentration of COOH-terminal fragments and the serum survival time of these fragments are much greater than those of the native, secreted whole molecule, PTH 1–84.
During the past 5 years there has been a great increase in our understanding of the chemistry, biosynthesis, secretion, and pathophysiology of parathyroid hormone (PTH). PTH has been shown by HAMILTON, COHN, KEMPER, and colleagues (HAMILTON et al., 1971; COHN et al., 1971; COHN et al., 1972; KEMPER et al., 1972) to be synthesized as a precursor with a molecular weight of approximately 12,000. Structural studies of the bovine proparathyroid hormone (pro-PTH) have shown six additional amino acid residues attached to the NH2-terminal end of the molecule (HAMILTON et al., 1973). Because amino acid compositional data suggest that pro-PTH has amino acid residues not yet accounted for by this hexapeptide sequence, the possibility that there is an additional sequence of amino acids attached to the COOH-terminal region is now being considered (HABENER et al., 1973; COHN et al., 1974). It is likely that the pro-PTH is converted, in the parathyroid gland, to the storage form of the hormone, an 84 amino acid polypeptide with a molecular weight of 9,500. It is not known presently if pro-PTH is released into the circulation.
The 84 amino acid polypeptide is a major form of the hormone secreted by the gland into the circulation after appropriate physiologic stimuli (HABENER et al., 1971). However, recent studies by a number of groups (HABENER et al., 1973; BERSON and YALOW, 1968; ARNAUD et al., 1970; ARNAUD et al., 1971a; CANTERBURY and REISS, 1972; HABENER et al., 1972; SEGRE et al., 1972; ARNAUD et al., 1973; SILVERMAN and YALOW, 1973; ARNAUD,1973a; ARNAUD et al., in press; SEGRE et al., in press) indicate that there are multiple forms of the hormone in the blood of patients with hyperparathyroidism. These multiple forms include the 84 amino acid polypeptide and fragments of it. The major fragment has a molecular weight of 6,000 to 7,000, is COOH-terminal, and has a very long half-life. Canterbury et al. (1973) have demonstrated that a fragment(s) in the plasma of hyperparathyroid patients was biologically active in the renal adenylate cyclase system, which suggests that some of the circulating plasma fragments retain biologic activity. Indisputable immunologic evidence of this latter observation is still lacking, although it is supported by work, presented in the present paper, using an antiserum of great specificity for NH2-terminal PTH.
The present paper describes recent studies on the chemistry of human, bovine, and porcine PTH as well as some partical consequences of the immunoheterogeneity of PTH in serum when this radioimmunoassay is used in the routine evaluation of patients with hyperparathyroidism.
KeywordsAdenoma Lysine Polypeptide Glutamine Methionine
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- ARNAUD, C.D.: Immunochemical heterogeneity of circulating parathyroid hormone in man: sequel to an original observation by Berson and Yalow. Mt. Sinai J. Med. NY. 40, 422–432, 1973b.Google Scholar
- ARNAUD, C.D., GOLDSMITH, R.S., SIZEMORE, G.W., OLDHAM, S.B., BISCHOFF, J., LARSEN, J.A., BORDIER, P.: Studies on characterization of human parathyroid hormone in hyperparathyroid serum: practical considerations. Excerpta Medica International Congress Series 270, 281–290 (1973).Google Scholar
- COHN, D.V., MACGREGOR, R.R., CHU, L.L.H., HAMILTON, J.W.: Studies on the biosynthesis in vitro of parathyroid hormone and other calcemic polypeptides of the parathyroid gland. Excerpta Medica International Congress Series 243, 173–182 (1971).Google Scholar
- FAIRWELL, T., BREWER, H.B.,Jr.: Identification by chemical ionization mass spectrometry (CI-MS) of the phenyl (PTH) and methyl (MTH) thiohydantion and thiazolinone amino acids obtained during the automated Edman degradation of polypeptides and proteins (abstract). Fed. Proc. 32, 648 (1973).Google Scholar
- HAMILTON, J.W., NIALL, H.D., KEUTMANN, H.T., POTTS, J.T.,Jr., COHN, D.V.: Amino terminal sequence of bovine proparathyroid hormone (calcemic fraction A) (abstract). Fed. Proc. 32, 269 (1973).Google Scholar
- POTTS, J.T. Jr., KEUTMANN, H.T., NIALL, H.D., TREGEAR, G.W., HABENER, J.F., O’RIORDAN, J.L.H., MURRAY, T.M., POWELL, D., AURBACH, G.D.: Parathyroid hormone: chemical and immunochemical studies of the active molecular species. In: Endocrinology 1971 (Proceedings of the Third International Symposium) Edited by S. Taylor. pp 333–349. London: William Heinemann 1972.Google Scholar
- POTTS, J.T.,Jr., NIALL, H.D., TREGEAR, G.W., VAN RIETSCHOTEN, J., HABENER, J.F., SEGRE, G.V., KEUTMANN, H.T.: Chemical and biologic studies of proparathyroid hormone and parathyroid hormone: analysis of hormone biosynthesis and metabolism. Mt. Sinai. J. Med. NY. 40, 448–461 (1973).Google Scholar
- REISS, E., CANTERBURY, J.M.: A radioimmunoassay for parathyroid hormone in man. Proc. Soc. Exp. Biol. Med. 128, 501–504 (1968).Google Scholar