The Role of Lysyl Residue in Collagen Structure with Calcification and Aging

  • M. Igarashi
  • Y. Hayashi
  • M. Matsuura
  • S. Yoshino
Conference paper

Abstract

Collagen comprises about 90% of bone matrix. There are conflicting theories of calcification mechanism. Ultrastructural studies of cartilage and bone matrix revealed the cellular process of initial calcification (BONUCCI, 1967). On the other hand, from the epitactic theory by NEUMAN and NEUMAN (1953) to the charge neutralization theory by URRY (1971), the major role of collagen remains that of inducing mineralization. Crosslinks of collagen occur following the enzymatic conversion of the lysyl or hydroxylysyl residue in peptide linkage to aldehyde. The degree of crosslinks may affect the number of residues of lysine or hydroxylysine obtained from collagen hydrolysate.

Keywords

Hydrolysis Osteoporosis Citrate Aldehyde Proline 

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References

  1. BLUMENKRANTZ, N., PROCKOP, J.: Quantitative assay for hydroxylysine in protein hydrolyzates. Anal. Biochem. 39, 59–64 (1971).PubMedCrossRefGoogle Scholar
  2. BONUCCI, E.: Fine structure of early cartilage calcification. J. Ultrastruct. Res. 20, 33–45 (1967).PubMedCrossRefGoogle Scholar
  3. DAVIS, N.R.: A method for the determination of lysine-derived collagen crosslinks. Biochem. Biophys. Res. Commun. 52, 877–883 (1973).PubMedCrossRefGoogle Scholar
  4. HAYASHI, Y., IGARASHI, M., YOSHINO. S.: The amount of lysine from bone collagen in experimental osteoporosis. Bone Metabolism. 6, 81–84 (1973a).Google Scholar
  5. HAYASHI, Y., TAKIZAWA, H., IGARASHI, M.: Aging of bone 11/Collagen in the senile osteoporotic bone. Jap. J. Geriatrics. 10, 185 - 190(1973b).CrossRefGoogle Scholar
  6. NEUMAN, W.F., NEUMAN, M.W.: Nature of the mineral phase in bone. Chem. Rev. 53, 1–36 (1953).CrossRefGoogle Scholar
  7. URRY, D.W.: Neutral sites for calcium ion binding to elastin and collagen: A charge neutralization theory for calcification and its relationship to atherosclerosis. Proc. Nat. Acad. Sci. U.S.A. 68, 810–814 (1971).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1975

Authors and Affiliations

  • M. Igarashi
  • Y. Hayashi
  • M. Matsuura
  • S. Yoshino

There are no affiliations available

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