Abstract
The subunit relationships in glycogen phosphorylase, the interaction of this enzyme with phosphorylase kinase and its behaviour on “glycogen particles” have been studied. Energy transfer between fluorescent labels attached to SH-groups on different subunits has been used to describe the relationship between subunits across the dimer and tetramer interfaces. The spectroscopic properties of both fluorescent and spin-labels attached to phosphorylase respond to ligand interactions with the enzyme. Similarly conversion of phosphoyrlase b to a gives rise to spectroscopic changes which have been used to follow the kinetics of several events associated with activation. Spin-labelled phosphorylase b interacts with glycogen particles and the ESR spectrum of the label monitors ligand and protein interactions during “flash-activation”.
Contribution from the Oxford Enzyme Group.
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Abbreviations
- NBD-:
-
4-nitrobenzo-2-oxa-1, 3-diazole-group
References
Avramovic-Zikiv, O., Smillie, L. B., and Madsen, B. B., (1970), J. Biot. Chem., 245, 1558–1565.
Bennick, A., Campbell, I. D., Dwek, R. A., Price, N. C., Radda, G. K., and Salmon, A. G., (1971), Nature, New Biol., 234, 140–143.
Birkett, D. J., Radda, G. K., and Salmon, A. G., (1970), FEBS Letters, 11, 295–297.
Birkett, D. J., Dwek, R. A., Radda, G. K., Richards, R. E., and Salmon, A. G., (1971), Europ. J. Biochem., 20, 494–508.
Campbell, I. D., Dwek, R. A., Price, N. C., and Radda, G. K., (1972), Europ. J. Biochem., 30, 339–347.
Dwek, R. A., Radda, G. K., Richards, R. E., and Salmon, A. G., (1972a), Europ. J. Biochem., 29, 509–514.
Dwek, R. A., Griffiths, J. R., Strauss, U., and Radda, G. K., (1972b), FEBS Letters, 28, 161–164.
Förster, T., (1959), Disc. Faraday Soc., 27, 7–27.
Gaspar, R., and Damjanovitch, S., (1973), Biochim. Biophys. Acta., 315, 191–194.
Heilmeyer, L., Meyer, F., Haschke, R. H., and Fischer, E. H., (1970), J. Biol. Chem., 245, 6649–6656.
Hurd, S., Teller, D., and Fischer, E. G., (1966), Biochem. Biophys. Res. Commun., 24, 79–88.
Meyer, F., Heilmeyer, L., Haschke, R. H., and Fischer, E. H., (1970), J. Biol. Chem., 245, 6642–6648.
Radda, G. K., and Dwek, R. A., (1972), in Protein-Protien Interactions, Jaenicke, R., and Helmreich, E., Eds., Berlin, Springer-Verlag, p. 213–243.
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© 1974 Springer-Verlag Berlin · Heidelberg
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Brooks, D.J., Busby, S.J.W., Dwek, R.A., Griffiths, J.R., Radda, G.K. (1974). Studies on the Molecular Aspects of Phosphorylase b → a Conversion. In: Metabolic Interconversion of Enzymes 1973. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80817-3_2
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DOI: https://doi.org/10.1007/978-3-642-80817-3_2
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