Abstract
Before initiating quantitative mass determination of biological objects in one’s own laboratory, the following prerequisites must be available:
-
Electron Microscope
Any electron microscope is suitable for quantitative work.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literature
Anderson, T.: Techniques for the preservation of three-dimensional structure in preparing specimens for the electron microscope. Trans. N.Y. Acad. Sci., Ser. II, 13, 130–134 (1951).
Bahr, G.F.: Quantitative electron microscopy: The present state and a look into the future. Invited lecture, Fourth European Regional Conference on Electron Microscopy, Rome, Italy, 567–572, 1968.
Bahr, G.F.: Human chromosome fibers, considerations of DNA protein packing and of looping patterns. Exp. Cell Res. 62, 39–49 (1970).
Bahr, G.F., Carlsson, L., Zeitler, E.: Determination of the dry weight in populations of sub- microscopic particles by means of quantitative electron microscopy. First International Biophysics Congress, Stockholm, 1961, p. 327.
Bahr, G.F., Sackerlotzky, O., Zeitler, E.: Electromagnetic exposure system for Siemens Electron Microscope. Rev. Sci. Instr. 34, 1443–1444 (1963).
Bahr, G.F., Zeitler, E.: Study of bull spermatozoa, quantitative electron microscopy. J. Cell Biol. 21, 175–189 (1964).
Bahr, G.F., Zeitler, E.: The determination of dry mass in populations of isolated particles. Symposium on Quantitative Electron Microscopy (editors: Bahr, G.F., and Zeitler, E.), p. 217–239. Baltimore, Md.: Williams and Wilkins 1965.
Fernandez-Moran, H.: Examination of brain tumor tissue with the electron microscope. Ark. Zool. (Stockh.) 40, 1–15 (1948).
Gall, J.G.: Chromosome fibers studied by a spreading technique. Chromosoma (Berl.) 20, 221–233 (1966).
Hall, C.E.: Introduction to electron microscopy, 2nd ed. New York: McGraw-Hill 1966.
Halliday, J.S., Quinn, T.F.J.: Contrast of electron micrographs. Brit. J. Appl. Phys. 11, 486–491 (1960).
Kleinschmidt, A.K., Lang, D., Jacherts, D., Zahn, R.K.: Preparation and length measurements of the total desoxyribonucleic acid contant of T2 bacteriphages. Biochim. Biophys. Acta (Amst.) 61, 857–864 (1962).
Lippert, W.: Erfahrungen mit der photographischen Methode bei der Massendickenbestimmung im Elektronenmikroskop. Optik 29, 273–278 (1969).
Reimer, L.: Elektronenmikroskopische Untersuchungs- und Präparationsmethoden, 2nd ed. Berlin- Heidelberg-New York: Springer 1967.
Silverman, L., Glick, D.: Measurement of protein concentration by quantitative electron microscopy. J. Cell Biol. 40, 773–778 (1969).
Silverman, L., Schreiner, B., Glick, D.: Measurement of thickness within sections by quantitative electron microscopy. J. Cell Biol. 40, 768–772 (1969).
Silvester, N.R., Burge, R.E.: A method for the routine evaluation of dry mass from electron micrographs. J. Roy. Micr. Soc. 81, 25 (1962).
Smith, G.H., Burge, R.E.A.: Theoretical investigation of plural and multiple scattering of electrons by amorphous films, with special reference to image contrast in the electron microscope. Proc. Phys. Soc. 81, 612 (1963).
Zeitler, E., Bahr, G.F.: A photometric procedure for weight determination of submicroscopic particle by quantitative electron microscopy. J. Appl. Phys. 33, 847–853 (1962).
Zeitler, E., Bahr, G.F.: Contrast and mass thickness. Symposium on quantitative electron microscopy (editors: Bahr, G.F., and Zeitler, E.), p. 208–216. Baltimore, Md.: Williams and Wilkins 1965.
Wied, G.L., Bibbo, M., Bahr, G.F., Bartels, P.H.: Computerized microdissection of cellular images. Acta Cytol. (Philad.) 14, 418–433 (1970).
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1973 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Neuhoff, V. (1973). Determination of the Dry Mass of Small Biological Objects by Quantitative Electron Microscopy. In: Micromethods in Molecular Biology. Molecular Biology Biochemistry and Biophysics, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80761-9_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-80761-9_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-80763-3
Online ISBN: 978-3-642-80761-9
eBook Packages: Springer Book Archive