Micro-Electrophoresis for RNA and DNA Base Analysis

  • Volker Neuhoff
Part of the Molecular Biology Biochemistry and Biophysics book series (MOLECULAR, volume 14)


Electrophoresis in a microscopic fiber (microphoresis) separates nucleic acid components in amounts corresponding to 500–1000 pg RNA (Edstrom 1960 a, 1964 a). This sensitivity permits RNA base analysis at the cellular level. In a scaled- up, somewhat simpler version, cellophane strips are used as the supporting medium (Koenig and BrattgArd, 1963; Ruchel, 1971) for the analysis of 3 000–5 000 pg RNA. Microphoresis in cellophane strips was introduced by Koenig and BrattgArd for the quantitative determination of radioactively labelled RNA, which, with the technique employed, required more RNA than is normally used for fibers. These techniques also lend themselves to base analysis of DNA and, with a simple modification, to the simultaneous determination of DNA content and base composition (Edstrom, 1964 b). Microelectrophoresis can also be used for enzyme determinations, in which substrate and product are separated after incubation with micro-isolated components, as used for intracellular localization of nucleases in starfish oocytes (Sierakowska, Edstrom, and Shugar, 1964).


Methylene Blue Liquid Paraffin Lampbrush Chromosome Quartz Slide Lever Control 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Althaus, H.-H., Briel, G., Dames, W., Neuhoff, V.: Zelluläre und moleculare Grundlagen der nervösen Erregungsspeicherung. 2. Neurochemische Mikroanalysen des Rückenmarks der Katze nach posttetanischer Potenzierung monosynaptischer Reflexe. In: Sonderforschungsbereich 33, Nervensystem und biologische Information, Göttingen 1969–1972, p. 107–121.Google Scholar
  2. Andersson, E., Edström, A., Jarlstedt, J.: Properties of RNA from giant axons of the crayfish. Acta physiol. scand. 78, 491–502 (1970).PubMedCrossRefGoogle Scholar
  3. Daneholt, B., Brattgård, S.-O.: A comparison between RNA metabolism of nerve cells and glia in the hypoglossal nucleus of the rabbit. J. Neurochem. 13, 913–921 (1966).PubMedCrossRefGoogle Scholar
  4. Daneholt, B., Edström, J.-E.: The content of deoxyribonucleic acid in individual polytene chromosomes of Chironomus tentans. Cytogenetics 6, 350–356 (1967).PubMedCrossRefGoogle Scholar
  5. Daneholt, B., Edström, J.-E.: The DNA base composition of individual chromosomes and chromosome segments from Chironomus tentans. J. Cell Biol. 41, 620–624 (1969).PubMedCrossRefGoogle Scholar
  6. Eccles, J.-C., McIntyre, A.K.: The effects of disuse and of activity on mamalian spinal reflexes. J. Physiol. (Lond.) 121, 492–516 (1953).Google Scholar
  7. Edström, J.-E.: Ribonucleic acid mass and concentration in individual nerve cells. A new method for quantitative determinations. Biochim. biophys. Acta (Amst.) 12, 361–386 (1953).CrossRefGoogle Scholar
  8. Edström, J.-E.: Extraction, hydrolysis, and electrophoretic analysis of ribonucleic acid from microscopic tissue units (microphoresis). J. biophys. biochem. Cyt. 8, 39–43 (1960a).CrossRefGoogle Scholar
  9. Edström, J.-E.: Composition of ribonucleic acid from various parts of spider oocytes. J. biophys. biochem. Cyt. 8, 47–51 (1960b).CrossRefGoogle Scholar
  10. Edström, J.-E.: Microextraction and microelectrophoresis for determination and analysis of nucleic acids in isolated cellular units. In: Methods in cell physiology I, ed. by D.M. Prescott, p. 417–447. New York: Academic Press 1964a.Google Scholar
  11. Edström, J.-E.: Microelectrophoretic determination of deoxyribonucleic acid content and base composition in microscopic tissue samples. Biochim. biophys. Acta (Amst.) 80, 399–410 (1964b).Google Scholar
  12. Edström, J.-E., Beermann, W.: The base composition of nucleic acids in chromosomes, puffs, nucleoli and cytoplasm of Chironomus salivary gland cells. J. Cell Biol. 14, 371–380 (1962).PubMedCrossRefGoogle Scholar
  13. Edström, J.-E., Gall, J.G.: The base composition of ribonucleic acid in lampbrush chromosomes, nucleoli, nuclear sap and cytoplasm of Triturus oocytes. J. Cell Biol. 19, 279–284 (1963).CrossRefGoogle Scholar
  14. Edström, J.-E., Grampp, W., Schor, N.: The intracellular distribution and heterogeneity of ribonucleic acid in starfish oocytes. J. biophys. biochem. Cytol. 11, 549–557 (1961).PubMedCrossRefGoogle Scholar
  15. Edström, J.-E., Kawiak, J.: Microchemical deoxyribonucleic acid determination in individual cells. J. biophys. biochem. Cytol. 9, 619–626 (1961).PubMedCrossRefGoogle Scholar
  16. Edström, J.-E., Pilhage, L.: Extraction, hydrolysis, and electrophoretic analysis of ribonucleic acid from microscopic tissue units (microphoresis). J. biophys. biochem. Cytol. 8, 44–46 (1960).PubMedGoogle Scholar
  17. Egyházi, E., Hydén, H.: RNA with high specific activity in neurons and glia. Brain Res. 2, 197–200 (1966).PubMedCrossRefGoogle Scholar
  18. Hydén, H.: Quantitative assay of compounds in isolated fresh, nerve cells and glial cells from control and stimulated animals. Nature (Lond.) 184, 433–435 (1959).CrossRefGoogle Scholar
  19. Hydén, H.: The neuron. In: The cell, ed. by J. Brachet and A. Mirsky, vol. IV, p. 215. New York: Academic Press 1960.Google Scholar
  20. Hydén, H.: Satellite cells in the nervous system. Sci. Amer. 205, 62–70 (1961).PubMedCrossRefGoogle Scholar
  21. Hydén, H.: Biochemical and functional interplay between neuron and glia. In: Recent advances in biological psychiatry, ed. by J. Wortis, vol. VI, p. 31–54. New York, London: Plenum Publ. Corp. 1963.Google Scholar
  22. Hydén, H.: Behavior, neural function and RNA. In: Progress in nucleic acid research and molecular biology, ed. by J.N. Davison and W.E. Cohn, vol. 6, p. 187–218. New York, London: Academic Press 1967.Google Scholar
  23. Hydén, H., Egyházi, E.: Changes in RNA content and base composition in cortical neurons of rats in a learning experiment involving transfer of handedness. Proc. nat. Acad. Sci. (Wash.) 52, 1030– 1035 (1964).CrossRefGoogle Scholar
  24. Jarlstedt, J., Mytilineo, C.: Effect of reserpine on sympathetic neuronal RNA. Brain Res. 28, 355–356 (1971).PubMedCrossRefGoogle Scholar
  25. Koenig, E., Brattgård, S.-O.: A quantitative micromethod for determination of specific radioactivity of H3-purines and H3-pyrimides. Analyt. Biochem. 6, 424 (1963).PubMedCrossRefGoogle Scholar
  26. Neuhoff, V.: Präparation der Neuroglia zur Mikroelektrophorese der Ribonucleinsäure-Basen. Arzneimittel-Forsch. 16, 779–781 (1966).Google Scholar
  27. Neuhoff, V.: Die Wirkung von Lysergsäurediäthylamid auf Ganglienzellen. 1. Mitt. Arbeitshypothese und Ribonucleinsäure-Basen im Hippocampus. Arzneimittel-Forsch. 17, 176–181 (1967).Google Scholar
  28. Neuhoff, V., Müller, D., Ter Meulen, V.: Präparation von Ganglienzellen für cytophotometrische Untersuchungen. Z. wiss. Mikr. 69, 65–72 (1968).PubMedGoogle Scholar
  29. Neuhoff, V.: Wet weight determination in the lower milligram range. Analyt. Biochem. 41, 270–271 (1971).PubMedCrossRefGoogle Scholar
  30. Ringborg, U.: Composition and content of RNA in neurons of rat hippocampus at different ages. Brain Res. 2, 296–298 (1966).PubMedCrossRefGoogle Scholar
  31. Rüchel, R.: Mikroelektrophoresen von RNS-Basen. Anwendung zur Untersuchung bestimmter Hirnregionen und kritische Analyse der Methode. Inaugural-Dissertation Göttingen, 1971.Google Scholar
  32. Rüchel, R., Rehmann, U., Neuhoff, V.: In preparation.Google Scholar
  33. Sierakowska, H., Edström, J.-E., Shugar, D.: Intracellular localization of nuclease enzymes by a microdissection-microelectrophoretic technique. Acta biochim. pol. 2, 497–507 (1964).Google Scholar
  34. Ter Meulen, V., Müller, D., Neuhoff, V., Joppich, G.: Immunhistological, microscopical and neurochemical studies on encephalitides. V. Subacute sclerosing panencephalitis. Cytophotometric studies on isolated nerve cells. Acta neuropath. (Berl.) 15, 128–141 (1970).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1973

Authors and Affiliations

  • Volker Neuhoff
    • 1
  1. 1.Medizin (Arbeitsgruppe Neurochemie)Max-Planck-Institut für ExperimentelleGöttingenGermany

Personalised recommendations