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Micro-Diffusion Techniques

  • Volker Neuhoff
Part of the Molecular Biology Biochemistry and Biophysics book series (MOLECULAR, volume 14)

Abstract

Two-dimensional micro-immunoprecipitation is equivalent to the Ouchter- lony test used on the macro-scale. The theoretical basis of immunoprecipitation and descriptions of the current macro procedures may be obtained from the appropriate text books (e.g. Stafseth, Stockton and Newman, 1956; Kabat and Mayer, 1961; Campbell et al, 1963; Ackroyd, 1964; Steffen, 1968; Williams and Chase, 1967, 1968, 1971). The micro-method has the advantage that it can be performed with extremely small quantities of material and, because of the very short diffusion distances, the result is obtained rapidly. For example, on the macro-scale the test takes 24 to 48 hrs. whereas the micro-method takes several minutes to a few hours.

Keywords

Radial Diffusion Moist Chamber Barbital Buffer Agarose Solution Agarose Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1973

Authors and Affiliations

  • Volker Neuhoff
    • 1
  1. 1.Medizin (Arbeitsgruppe Neurochemie)Max-Planck-Institut für ExperimentelleGöttingenGermany

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