The purification of very small quantities of protein solutions by dialysis is a frequent problem. Fig. 1 illustrates two dialysis chambers made of Dynal (Polyoxymethylene) which are suitable for the dialysis of 100 μl or 15 μl respectively. A 4 mm, or 2 mm, thick disc (external diameter ca. 20 mm) with a central hole which may measure between 1 mm and 6 mm according to the volumes to be dialysed (15, 30, 50, 100, 250, 500 μl) is first fitted on one side with a suitable semi-permeable membrane, and a similarly bored rubber washer1 and a sealing cap are screwed on in such a way that the central hole is sealed only by the permeable membrane. The membrane is moistened in a suitable buffer before use.
KeywordsCentral Hole Equilibrium Dialysis Thick Disc Frequent Problem Screw Thread
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- Neuhoff, V., Kiehl, F.: Dialysiergeräte für Volumen zwischen 10 und 500 μl. Arzneimittel-Forsch. (Drug Res.) 19, 1898–1899 (1969).Google Scholar