Advertisement

Micro-Dialysis

  • Volker Neuhoff
Chapter
Part of the Molecular Biology Biochemistry and Biophysics book series (MOLECULAR, volume 14)

Abstract

The purification of very small quantities of protein solutions by dialysis is a frequent problem. Fig. 1 illustrates two dialysis chambers made of Dynal (Polyoxymethylene) which are suitable for the dialysis of 100 μl or 15 μl respectively. A 4 mm, or 2 mm, thick disc (external diameter ca. 20 mm) with a central hole which may measure between 1 mm and 6 mm according to the volumes to be dialysed (15, 30, 50, 100, 250, 500 μl) is first fitted on one side with a suitable semi-permeable membrane, and a similarly bored rubber washer1 and a sealing cap are screwed on in such a way that the central hole is sealed only by the permeable membrane. The membrane is moistened in a suitable buffer before use.

Keywords

Central Hole Equilibrium Dialysis Thick Disc Frequent Problem Screw Thread 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature

  1. Neuhoff, V., Kiehl, F.: Dialysiergeräte für Volumen zwischen 10 und 500 μl. Arzneimittel-Forsch. (Drug Res.) 19, 1898–1899 (1969).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1973

Authors and Affiliations

  • Volker Neuhoff
    • 1
  1. 1.Medizin (Arbeitsgruppe Neurochemie)Max-Planck-Institut für ExperimentelleGöttingenGermany

Personalised recommendations