Skip to main content
SpringerLink
Log in

Molecular Aspects of the Regulation of the Mammalian Pyruvate Dehydrogenase Complex

  • Conference paper
Metabolic Interconversion of Enzymes

Abstract

Pyruvate dehydrogenase systems have been isolated from Escherichia coli, avian and mammalian tissues, and Neurospora crassa as functional units with molecular weights in the millions (1). The bacterial, avian, and mammalian pyruvate dehydrogenase complexes have been separated into three enzymes - pyruvate dehydrogenase, dihydrolipoyl transacetylase, and dihydrolipoyl dehydrogenase (a flavoprotein), and functional units resembling the native complexes have been reassembled from the individual enzymes. These three enzymes act in a coordinated manner as indicated in Fig. 1 (1, 2). The discussion will be limited to the structure, function, and regulation of the mammalian pyruvate dehydrogenase complex.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 54.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 69.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. REED, L. J., in “Current Topics in Cellular Regulation” (B.L. Horecker and E.R. Stadtman, eds.), Vol. 1, p. 233, Academic Press, New York, 1969.

    Google Scholar 

  2. GUNSALUS, I.C., in “The Mechanism of Enzyme Action” (W.B. McElroy and H.B. Glass, eds.), p. 545, Johns Hopkins Press, Baltimore, 1954.

    Google Scholar 

  3. LINN, T.C., PELLEY, J.W., PETTIT, F.H., HUCHO, F., RANDALL, D.D., and REED, L.J., Arch. Biochem. Biophys., in press.

    Google Scholar 

  4. SMOLY, J.M., KUYLENSTIERNA, B., and ERNSTER, L., Proc. Nat. Acad. Sci. U.S.A., 66, 125 (1970).

    Article  CAS  Google Scholar 

  5. ISHIKAWA, E., OLIVER, R.M., and REED, L.J., Proc. Nat. Acad. Sci. U.S.A., 56, 534 (1966).

    Article  CAS  Google Scholar 

  6. HAYAKAWA, T., KANZAKI, T., KITAMURA, T., FUKUYOSHI, Y., SAKURAI, Y., KOIKE, K., SUEMATSU, T., and KOIKE, M., J. Biol. Chem., 244, 3660 (1969).

    CAS  Google Scholar 

  7. BARRERA, C.R., NAMIHIRA, G., HAMILTON, L., MUNK, P., ELEY, M.H., LINN, T.C., and REED, L.J., Arch. Biochem. Biophys., in press.

    Google Scholar 

  8. LINN, T.C., PETTIT, F.H., and REED, L.J., Proc. Nat. Acad. Sci. U.S.A.. 62, 234 (1969).

    Article  CAS  Google Scholar 

  9. MASSEY, V., HOFMANN, T., and PALMER, G., J. Biol. Chem.. 237. 3820 (1962).

    CAS  Google Scholar 

  10. SAKURAI, Y., FUKUYOSHI, Y., HAMADA, M., HAYAKAWA, T., and KOIKE, M., J. Biol. Chem.. 245, 4453 (1970).

    CAS  Google Scholar 

  11. LINN, T.C., PETTIT, F.H., HUCHO, F., and REED, L.J., Proc. Nat. Acad. Sci. U.S.A.. 64, 227 (1969).

    Article  CAS  Google Scholar 

  12. GARLAND, P.B., and RANDLE, P.J., Biochem. J., 91, 6c (1964).

    CAS  Google Scholar 

  13. BREMER, J., Eur. J. Biochem., 8, 535 (1969).

    Article  CAS  Google Scholar 

  14. WIELAND, O., von JAGOW-WESTERMANN, B., and STUKOWSKI, B., Hoppe-Seyler’s Z. Physiol. Chem., 350. 329 (1969).

    Article  CAS  Google Scholar 

  15. WIELAND, O., and von JAGOW-WESTERMANN, B., FEBS Letters. 3, 271 (1969).

    Article  CAS  Google Scholar 

  16. WIELAND, O., and SIESS, E., Proc. Nat. Acad. Sci. U.S.A.. 65, 947 (1970).

    Article  CAS  Google Scholar 

  17. JUNGAS, R.L., Metabolism. 20, 43 (1971).

    Article  CAS  Google Scholar 

  18. SIESS, E., WITTMANN, J., and WIELAND, O., Hoppe-Seyler’s Z. Physiol. Chem.. 352, 447 (1971).

    Article  CAS  Google Scholar 

  19. DENTON, R.M. COORE, H.G., MARTIN, B.R., and RANDLE, P.J., Nature New Biology, 231., 115 (1971).

    Article  CAS  Google Scholar 

  20. REED, L.J., LINN, T.C., PETTIT, F.H., OLIVER, R.M., HUCHO, F., PELLEY, J.W., RANDALL, D.D., and ROCHE, T.E., in “Energy Metabolism and the Regulation of Metabolic Processes in Mitochondria” (M.A. Mehlman and R.W. Hanson, eds.), Academic Press, New York, in press.

    Google Scholar 

  21. HUTCHESON, E.T., BROWN, J.R., and REED, L.J., in preparation.

    Google Scholar 

  22. ROCHE, T.E., and REED, L.J., in preparation.

    Google Scholar 

  23. VOGEL, O., and HENNING, U., Eur. J. Biochem., 18, 103 (1971).

    Article  CAS  Google Scholar 

  24. NAMIHIRA, G., HAMILTON, L., MUNK, P., COX, D.J., and REED, L.J., in preparation.

    Google Scholar 

  25. SCHWARTZ, E.R., and REED, L.J., Biochemistry. 9, 1434 (1970).

    Article  CAS  Google Scholar 

  26. HUCHO, F., RANDALL, D.D., ROCHE, T.E., BURGETT, M.W., and REED, L.J., in preparation.

    Google Scholar 

  27. BOGUCKA, K., and WOJTCZAK, L., Biochem. Biophys. Res. Commun., 44. 1330 (1971).

    Article  CAS  Google Scholar 

  28. WIELAND, O., SIESS, E., SCHULZE-WETHMAR, F.H., von FUNCKE, H.G., and WINTON, B., Arch. Biochem. Biophys.. 143. 593 (1971).

    Article  CAS  Google Scholar 

  29. WEISS, L., LOFFLER, G., SCHIRMANN, A., and WIELAND, O., FEBS Letters, 15. 229, (1971).

    Article  CAS  Google Scholar 

  30. SÖLING, H.D., and BERNHARD, G., FEBS Letters. 13, 201 (1971).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Clayton Foundation Biochemical Institute and Department of Chemistry, The University of Texas at Austin, Austin, Texas, USA

    Lester J. Reed, Tracy C. Linn, Ferdinand Hucho, Genshin Namihira, Cecilio R. Barrera, Thomas E. Roche, John W. Pelley & Douglas D. Randall

Authors
  1. Lester J. Reed
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tracy C. Linn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ferdinand Hucho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Genshin Namihira
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cecilio R. Barrera
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Thomas E. Roche
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. John W. Pelley
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Douglas D. Randall
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Diabetesforschung, 8000, Kölner Platz 1, München 23, GFR

    O. Wieland

  2. Physiologisch-chemisches Institut, Universität Würzburg, 8700, Koellikerstrasse 2, Würzburg, GFR

    E. Helmreich

  3. Biochemisches Institut, Universität Freiburg, 7800, Hermann-Herder-Strasse 7, Freiburg i.Br., GFR

    H. Holzer

Rights and permissions

Reprints and permissions

Copyright information

© 1972 Springer-Verlag Berlin · Heidelberg

About this paper

Cite this paper

Reed, L.J. et al. (1972). Molecular Aspects of the Regulation of the Mammalian Pyruvate Dehydrogenase Complex. In: Wieland, O., Helmreich, E., Holzer, H. (eds) Metabolic Interconversion of Enzymes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80698-8_20

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-80698-8_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-05919-6

  • Online ISBN: 978-3-642-80698-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation