Probleme der radioimmunologischen Eiweißhormonbestimmung
Problems in Radioimmunoassay of Peptide Hormones
Radioimmunoassay is specific and sufficiently sensitive to detect ng or pg amounts of peptide hormones. “Results” are easily obtained, but for a correct interpretation the following points have to be considered: Immunological reactivity of the labelled hormone, behaviour of damaged hormone and free iodine in the separation system used, standardisation of antibody and reference preparation of the hormone, non-specific factors interfering with the antigen-antibody reaction. Several dilutions of the unknown should always be assayed in order to control the specificity of the reaction.