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Probleme der radioimmunologischen Eiweißhormonbestimmung

  • J. Girard
  • J. B. Baumann
  • P. W. Nars
  • M. Vest
Part of the Symposion der Deutschen Gesellschaft für Endokrinologie in Ulm vom 26.—28. Februar 1970 book series (ENDOKRINOLOGIE, volume 16)

Problems in Radioimmunoassay of Peptide Hormones

Summary

Radioimmunoassay is specific and sufficiently sensitive to detect ng or pg amounts of peptide hormones. “Results” are easily obtained, but for a correct interpretation the following points have to be considered: Immunological reactivity of the labelled hormone, behaviour of damaged hormone and free iodine in the separation system used, standardisation of antibody and reference preparation of the hormone, non-specific factors interfering with the antigen-antibody reaction. Several dilutions of the unknown should always be assayed in order to control the specificity of the reaction.

Literatur

  1. Greenwood, F. C.; In: “Modern Trends in Endocrinology”, series 3, pp 288–322 Ed. H. Gardiner-Hill, London: Butterworth 1967.Google Scholar
  2. Berson, S. A., Yalow, R. S.: In “Clinical Endocrinology””II pp 699–720 Ed. E. B. Astwood and C. E. Cassidy, New York: Grune and Stratton 1968.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg New York 1970

Authors and Affiliations

  • J. Girard
    • 1
  • J. B. Baumann
    • 1
  • P. W. Nars
    • 1
  • M. Vest
    • 1
  1. 1.UniversitätskinderklinikBaselSchweiz

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