Die Wirkung von FSH und LH auf die Proteinsynthese verschiedener Organe des weiblichen neugeborenen Meerschweinchens

Summary

This study demonstrates that guinea pig ovary and uterus are sensitive to gonadotropic or oestrogenic stimulation already during the neonatal period. Uterine protein synthesis indicates an increase of nearly 100% as early as 1 hour after injection of 0,1 yg oestradiol/100 g body weight. 1 hour after subcutaneous application of 5 I.U. LH (NIH-LH-B 3) H³-leucine incorporation into ovarian protein is markedly elevated. The peak of this gonadotropic stimulation is seen after 6 hours. The tenfold dose of LH produces an earlier increase of protein synthesis but does not cause an additional elevation of amino acid incorporation. Furthermore LH effects the permeability of ovarian cells. After LH treatment a higher concentration of H³-leucine in the acid soluble supernatant is found. In another series of experiments also an activation of ovarian RNA-synthesis is demonstrable. The simultaneous increase of uterine protein and RNA synthesis shows a stimulation of endogenous oestrogen in the regulation of ovarian steroidogenesis.

The application of 7 I.U. FSH (NIH-FSH-S 3) per 100 g body weight resulted in an elevation of precursor incorporation into ovarian protein and RNA. This effect may be correlated to hormonal control of the morphogenesis of this organ. As uterine H³-leucine and H³-uridine incorporation rate is not changed by FSH in the intact animal, one can conclude that FSH alone is ineffective in the regulation of ovarian steroidogenesis.